Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2978 and BT2979 are separated by 4 nucleotides BT2979 and tRNA-Met are separated by 93 nucleotides tRNA-Met and BT2980 are separated by 139 nucleotides BT2980 and BT2981 are separated by 78 nucleotides
BT2978: BT2978 - transcriptional regulator (NCBI ptt file), at 3,756,562 to 3,757,026
BT2978
BT2979: BT2979 - conserved hypothetical protein, putative membrane protein (NCBI ptt file), at 3,757,031 to 3,757,342
BT2979
BT_t53: tRNA-Met - tRNA-Met (RefSeq), at 3,757,436 to 3,757,512
tRNA-Met
BT2980: BT2980 - transposase (NCBI ptt file), at 3,757,652 to 3,758,920
BT2980
BT2981: BT2981 - hypothetical protein (NCBI ptt file), at 3,758,999 to 3,759,865
BT2981
Position (kb)
3757
3758
3759 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3757.049 kb on + strand at 3757.120 kb on - strand, within BT2979 at 3757.130 kb on + strand, within BT2979 at 3757.131 kb on - strand, within BT2979 at 3757.133 kb on + strand, within BT2979 at 3757.134 kb on - strand, within BT2979 at 3757.167 kb on + strand, within BT2979 at 3757.170 kb on + strand, within BT2979 at 3757.236 kb on + strand, within BT2979 at 3757.236 kb on + strand, within BT2979 at 3757.237 kb on - strand, within BT2979 at 3757.353 kb on + strand at 3757.353 kb on + strand at 3757.354 kb on - strand at 3757.373 kb on - strand at 3757.383 kb on + strand at 3757.383 kb on + strand at 3757.383 kb on + strand at 3757.391 kb on + strand at 3757.539 kb on + strand at 3757.570 kb on + strand at 3757.570 kb on + strand at 3757.572 kb on + strand at 3757.573 kb on - strand at 3757.597 kb on - strand at 3757.597 kb on - strand at 3757.608 kb on - strand at 3757.622 kb on + strand at 3757.638 kb on - strand at 3757.648 kb on + strand at 3757.648 kb on + strand at 3757.652 kb on - strand at 3757.652 kb on - strand at 3757.664 kb on - strand at 3757.732 kb on - strand at 3757.753 kb on - strand at 3757.770 kb on + strand at 3757.785 kb on + strand, within BT2980 at 3757.789 kb on + strand, within BT2980 at 3757.789 kb on + strand, within BT2980 at 3757.789 kb on + strand, within BT2980 at 3757.790 kb on - strand, within BT2980 at 3757.822 kb on + strand, within BT2980 at 3757.823 kb on - strand, within BT2980 at 3757.823 kb on - strand, within BT2980 at 3757.823 kb on - strand, within BT2980 at 3757.834 kb on - strand, within BT2980 at 3757.834 kb on - strand, within BT2980 at 3757.837 kb on + strand, within BT2980 at 3757.853 kb on + strand, within BT2980 at 3757.853 kb on + strand, within BT2980 at 3757.870 kb on - strand, within BT2980 at 3757.882 kb on - strand, within BT2980 at 3757.882 kb on - strand, within BT2980 at 3757.911 kb on + strand, within BT2980 at 3757.911 kb on + strand, within BT2980 at 3757.912 kb on - strand, within BT2980 at 3757.962 kb on + strand, within BT2980 at 3757.983 kb on + strand, within BT2980 at 3757.984 kb on - strand, within BT2980 at 3757.990 kb on + strand, within BT2980 at 3757.991 kb on - strand, within BT2980 at 3757.993 kb on + strand, within BT2980 at 3758.016 kb on - strand, within BT2980 at 3758.016 kb on - strand, within BT2980 at 3758.050 kb on - strand, within BT2980 at 3758.054 kb on - strand, within BT2980 at 3758.071 kb on - strand, within BT2980 at 3758.071 kb on - strand, within BT2980 at 3758.078 kb on - strand, within BT2980 at 3758.085 kb on + strand, within BT2980 at 3758.085 kb on + strand, within BT2980 at 3758.162 kb on - strand, within BT2980 at 3758.236 kb on + strand, within BT2980 at 3758.264 kb on - strand, within BT2980 at 3758.305 kb on + strand, within BT2980 at 3758.305 kb on + strand, within BT2980 at 3758.306 kb on - strand, within BT2980 at 3758.317 kb on + strand, within BT2980 at 3758.374 kb on + strand, within BT2980 at 3758.375 kb on - strand, within BT2980 at 3758.375 kb on - strand, within BT2980 at 3758.394 kb on - strand, within BT2980 at 3758.395 kb on + strand, within BT2980 at 3758.478 kb on - strand, within BT2980 at 3758.478 kb on - strand, within BT2980 at 3758.485 kb on + strand, within BT2980 at 3758.485 kb on + strand, within BT2980 at 3758.488 kb on + strand, within BT2980 at 3758.507 kb on - strand, within BT2980 at 3758.509 kb on + strand, within BT2980 at 3758.509 kb on + strand, within BT2980 at 3758.510 kb on - strand, within BT2980 at 3758.510 kb on - strand, within BT2980 at 3758.514 kb on - strand, within BT2980 at 3758.514 kb on - strand, within BT2980 at 3758.540 kb on - strand, within BT2980 at 3758.565 kb on + strand, within BT2980 at 3758.580 kb on + strand, within BT2980 at 3758.580 kb on + strand, within BT2980 at 3758.603 kb on + strand, within BT2980 at 3758.604 kb on - strand, within BT2980 at 3758.612 kb on - strand, within BT2980 at 3758.639 kb on + strand, within BT2980 at 3758.668 kb on + strand, within BT2980 at 3758.668 kb on + strand, within BT2980 at 3758.669 kb on - strand, within BT2980 at 3758.718 kb on + strand, within BT2980 at 3758.732 kb on + strand, within BT2980 at 3758.735 kb on + strand, within BT2980 at 3758.735 kb on + strand, within BT2980 at 3758.736 kb on - strand, within BT2980 at 3758.736 kb on - strand, within BT2980 at 3758.736 kb on - strand, within BT2980 at 3758.759 kb on - strand, within BT2980 at 3758.759 kb on - strand, within BT2980 at 3758.784 kb on + strand, within BT2980 at 3758.793 kb on - strand, within BT2980 at 3758.793 kb on - strand, within BT2980 at 3758.794 kb on + strand at 3758.794 kb on + strand at 3758.794 kb on + strand at 3758.795 kb on - strand at 3758.813 kb on - strand at 3758.820 kb on + strand at 3758.821 kb on - strand at 3758.849 kb on + strand at 3758.869 kb on - strand at 3758.904 kb on - strand at 3758.915 kb on - strand at 3758.918 kb on + strand at 3758.926 kb on + strand at 3758.927 kb on - strand at 3758.960 kb on + strand at 3758.983 kb on + strand at 3758.999 kb on - strand at 3759.007 kb on + strand at 3759.023 kb on + strand at 3759.023 kb on + strand at 3759.028 kb on - strand at 3759.055 kb on + strand at 3759.056 kb on - strand at 3759.080 kb on - strand at 3759.101 kb on - strand, within BT2981 at 3759.106 kb on - strand, within BT2981 at 3759.145 kb on + strand, within BT2981 at 3759.172 kb on + strand, within BT2981 at 3759.182 kb on + strand, within BT2981 at 3759.188 kb on - strand, within BT2981 at 3759.218 kb on + strand at 3759.246 kb on - strand, within BT2981 at 3759.249 kb on - strand, within BT2981 at 3759.253 kb on + strand, within BT2981 at 3759.253 kb on + strand, within BT2981 at 3759.253 kb on + strand, within BT2981 at 3759.253 kb on + strand, within BT2981 at 3759.314 kb on + strand, within BT2981 at 3759.318 kb on + strand, within BT2981 at 3759.318 kb on + strand, within BT2981 at 3759.319 kb on - strand, within BT2981 at 3759.381 kb on + strand, within BT2981 at 3759.381 kb on + strand, within BT2981 at 3759.381 kb on + strand, within BT2981 at 3759.382 kb on - strand, within BT2981 at 3759.382 kb on - strand, within BT2981 at 3759.383 kb on + strand, within BT2981 at 3759.392 kb on - strand, within BT2981 at 3759.394 kb on + strand, within BT2981 at 3759.394 kb on + strand, within BT2981 at 3759.395 kb on - strand, within BT2981 at 3759.453 kb on - strand, within BT2981 at 3759.456 kb on + strand, within BT2981 at 3759.457 kb on - strand, within BT2981 at 3759.488 kb on - strand, within BT2981 at 3759.493 kb on + strand, within BT2981 at 3759.493 kb on + strand, within BT2981 at 3759.494 kb on - strand, within BT2981 at 3759.494 kb on - strand, within BT2981 at 3759.502 kb on - strand, within BT2981 at 3759.549 kb on + strand, within BT2981 at 3759.560 kb on + strand, within BT2981 at 3759.607 kb on + strand, within BT2981 at 3759.608 kb on - strand, within BT2981 at 3759.682 kb on + strand, within BT2981 at 3759.706 kb on - strand, within BT2981 at 3759.723 kb on + strand, within BT2981 at 3759.724 kb on - strand, within BT2981 at 3759.730 kb on - strand, within BT2981 at 3759.738 kb on + strand, within BT2981 at 3759.738 kb on + strand, within BT2981 at 3759.739 kb on - strand, within BT2981 at 3759.739 kb on - strand, within BT2981 at 3759.749 kb on - strand, within BT2981 at 3759.749 kb on - strand, within BT2981 at 3759.753 kb on + strand, within BT2981 at 3759.756 kb on + strand, within BT2981 at 3759.757 kb on - strand, within BT2981 at 3759.757 kb on - strand, within BT2981 at 3759.774 kb on - strand, within BT2981 at 3759.774 kb on - strand, within BT2981 at 3759.781 kb on + strand at 3759.799 kb on + strand at 3759.838 kb on + strand at 3759.839 kb on - strand at 3759.841 kb on + strand at 3759.841 kb on + strand at 3759.842 kb on - strand at 3759.863 kb on + strand at 3759.908 kb on + strand at 3759.909 kb on - strand at 3759.909 kb on - strand at 3759.913 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 3,757,049 + -1.8 3,757,120 - BT2979 0.29 +2.3 3,757,130 + BT2979 0.32 -2.5 3,757,131 - BT2979 0.32 -1.5 3,757,133 + BT2979 0.33 +2.7 3,757,134 - BT2979 0.33 -0.9 3,757,167 + BT2979 0.44 +0.7 3,757,170 + BT2979 0.45 +0.9 3,757,236 + BT2979 0.66 -1.2 3,757,236 + BT2979 0.66 +0.8 3,757,237 - BT2979 0.66 -2.1 3,757,353 + -2.0 3,757,353 + -0.4 3,757,354 - +0.2 3,757,373 - -1.8 3,757,383 + +0.4 3,757,383 + -1.1 3,757,383 + +0.1 3,757,391 + +0.7 3,757,539 + -1.7 3,757,570 + -3.4 3,757,570 + -3.5 3,757,572 + -2.6 3,757,573 - -3.3 3,757,597 - -0.3 3,757,597 - -2.7 3,757,608 - -1.8 3,757,622 + -0.6 3,757,638 - +0.4 3,757,648 + +0.1 3,757,648 + +3.3 3,757,652 - +0.1 3,757,652 - +0.7 3,757,664 - +0.4 3,757,732 - -2.1 3,757,753 - -0.9 3,757,770 + +0.5 3,757,785 + BT2980 0.10 +1.5 3,757,789 + BT2980 0.11 -4.4 3,757,789 + BT2980 0.11 -1.4 3,757,789 + BT2980 0.11 -0.7 3,757,790 - BT2980 0.11 +1.0 3,757,822 + BT2980 0.13 +1.7 3,757,823 - BT2980 0.13 +0.8 3,757,823 - BT2980 0.13 -1.0 3,757,823 - BT2980 0.13 +1.7 3,757,834 - BT2980 0.14 +0.3 3,757,834 - BT2980 0.14 -0.0 3,757,837 + BT2980 0.15 -1.1 3,757,853 + BT2980 0.16 +1.5 3,757,853 + BT2980 0.16 +0.7 3,757,870 - BT2980 0.17 +2.9 3,757,882 - BT2980 0.18 +0.6 3,757,882 - BT2980 0.18 +0.7 3,757,911 + BT2980 0.20 -2.9 3,757,911 + BT2980 0.20 +0.2 3,757,912 - BT2980 0.20 +0.7 3,757,962 + BT2980 0.24 -3.5 3,757,983 + BT2980 0.26 -0.8 3,757,984 - BT2980 0.26 -1.8 3,757,990 + BT2980 0.27 +0.0 3,757,991 - BT2980 0.27 +0.1 3,757,993 + BT2980 0.27 +2.7 3,758,016 - BT2980 0.29 -1.1 3,758,016 - BT2980 0.29 +1.0 3,758,050 - BT2980 0.31 -2.0 3,758,054 - BT2980 0.32 -2.5 3,758,071 - BT2980 0.33 +0.3 3,758,071 - BT2980 0.33 -2.5 3,758,078 - BT2980 0.34 -0.1 3,758,085 + BT2980 0.34 +0.7 3,758,085 + BT2980 0.34 +0.5 3,758,162 - BT2980 0.40 +0.7 3,758,236 + BT2980 0.46 +0.5 3,758,264 - BT2980 0.48 +0.2 3,758,305 + BT2980 0.51 +0.2 3,758,305 + BT2980 0.51 -0.8 3,758,306 - BT2980 0.52 +0.6 3,758,317 + BT2980 0.52 -0.1 3,758,374 + BT2980 0.57 +0.2 3,758,375 - BT2980 0.57 -0.2 3,758,375 - BT2980 0.57 +0.8 3,758,394 - BT2980 0.58 +0.1 3,758,395 + BT2980 0.59 +0.4 3,758,478 - BT2980 0.65 -1.4 3,758,478 - BT2980 0.65 +1.8 3,758,485 + BT2980 0.66 -1.4 3,758,485 + BT2980 0.66 -3.0 3,758,488 + BT2980 0.66 -2.2 3,758,507 - BT2980 0.67 -3.2 3,758,509 + BT2980 0.68 -2.6 3,758,509 + BT2980 0.68 -3.0 3,758,510 - BT2980 0.68 +1.7 3,758,510 - BT2980 0.68 +0.9 3,758,514 - BT2980 0.68 -1.4 3,758,514 - BT2980 0.68 +0.7 3,758,540 - BT2980 0.70 -2.4 3,758,565 + BT2980 0.72 +1.0 3,758,580 + BT2980 0.73 +0.1 3,758,580 + BT2980 0.73 -1.3 3,758,603 + BT2980 0.75 -0.0 3,758,604 - BT2980 0.75 +1.7 3,758,612 - BT2980 0.76 -2.1 3,758,639 + BT2980 0.78 -2.5 3,758,668 + BT2980 0.80 -1.0 3,758,668 + BT2980 0.80 +1.1 3,758,669 - BT2980 0.80 +0.3 3,758,718 + BT2980 0.84 +1.4 3,758,732 + BT2980 0.85 -0.0 3,758,735 + BT2980 0.85 +0.4 3,758,735 + BT2980 0.85 -2.0 3,758,736 - BT2980 0.85 +1.7 3,758,736 - BT2980 0.85 +0.1 3,758,736 - BT2980 0.85 +0.3 3,758,759 - BT2980 0.87 +1.3 3,758,759 - BT2980 0.87 +0.6 3,758,784 + BT2980 0.89 +0.3 3,758,793 - BT2980 0.90 -2.9 3,758,793 - BT2980 0.90 -3.6 3,758,794 + -2.7 3,758,794 + -2.0 3,758,794 + -2.1 3,758,795 - -2.1 3,758,813 - -2.6 3,758,820 + +2.1 3,758,821 - -2.3 3,758,849 + -0.5 3,758,869 - -3.9 3,758,904 - -1.8 3,758,915 - -2.6 3,758,918 + -3.6 3,758,926 + +1.0 3,758,927 - -1.6 3,758,960 + -1.8 3,758,983 + +0.3 3,758,999 - +0.4 3,759,007 + +0.4 3,759,023 + -2.2 3,759,023 + +0.2 3,759,028 - -0.2 3,759,055 + -0.3 3,759,056 - -0.9 3,759,080 - +2.7 3,759,101 - BT2981 0.12 +0.1 3,759,106 - BT2981 0.12 +0.1 3,759,145 + BT2981 0.17 -2.0 3,759,172 + BT2981 0.20 -0.6 3,759,182 + BT2981 0.21 -1.9 3,759,188 - BT2981 0.22 -1.2 3,759,218 + +0.7 3,759,246 - BT2981 0.28 +1.0 3,759,249 - BT2981 0.29 -1.1 3,759,253 + BT2981 0.29 +0.4 3,759,253 + BT2981 0.29 -0.1 3,759,253 + BT2981 0.29 -0.6 3,759,253 + BT2981 0.29 +0.2 3,759,314 + BT2981 0.36 -1.8 3,759,318 + BT2981 0.37 -2.0 3,759,318 + BT2981 0.37 -0.5 3,759,319 - BT2981 0.37 -0.6 3,759,381 + BT2981 0.44 +0.1 3,759,381 + BT2981 0.44 -3.1 3,759,381 + BT2981 0.44 -2.1 3,759,382 - BT2981 0.44 -1.5 3,759,382 - BT2981 0.44 +0.7 3,759,383 + BT2981 0.44 +1.7 3,759,392 - BT2981 0.45 +0.2 3,759,394 + BT2981 0.46 -1.1 3,759,394 + BT2981 0.46 +0.8 3,759,395 - BT2981 0.46 -0.4 3,759,453 - BT2981 0.52 +1.5 3,759,456 + BT2981 0.53 +0.6 3,759,457 - BT2981 0.53 +0.3 3,759,488 - BT2981 0.56 -2.6 3,759,493 + BT2981 0.57 -3.5 3,759,493 + BT2981 0.57 -0.5 3,759,494 - BT2981 0.57 -2.5 3,759,494 - BT2981 0.57 -0.2 3,759,502 - BT2981 0.58 -0.2 3,759,549 + BT2981 0.63 -1.5 3,759,560 + BT2981 0.65 -0.9 3,759,607 + BT2981 0.70 +0.8 3,759,608 - BT2981 0.70 +1.2 3,759,682 + BT2981 0.79 -1.0 3,759,706 - BT2981 0.82 -1.9 3,759,723 + BT2981 0.84 -1.8 3,759,724 - BT2981 0.84 -0.5 3,759,730 - BT2981 0.84 -1.6 3,759,738 + BT2981 0.85 +0.7 3,759,738 + BT2981 0.85 -1.8 3,759,739 - BT2981 0.85 +0.3 3,759,739 - BT2981 0.85 +1.7 3,759,749 - BT2981 0.87 -0.9 3,759,749 - BT2981 0.87 +1.0 3,759,753 + BT2981 0.87 -2.1 3,759,756 + BT2981 0.87 +1.7 3,759,757 - BT2981 0.87 -0.3 3,759,757 - BT2981 0.87 +0.4 3,759,774 - BT2981 0.89 +1.4 3,759,774 - BT2981 0.89 +0.1 3,759,781 + -0.0 3,759,799 + +0.1 3,759,838 + +1.2 3,759,839 - -0.8 3,759,841 + +1.1 3,759,841 + +0.9 3,759,842 - +0.9 3,759,863 + -0.1 3,759,908 + -4.1 3,759,909 - -1.5 3,759,909 - +0.1 3,759,913 - -1.5
Or see this region's nucleotide sequence