Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2811 and BT2812 are separated by 64 nucleotides BT2812 and BT2813 are separated by 182 nucleotides BT2813 and BT2814 are separated by 24 nucleotides
BT2811: BT2811 - alkyl hydroperoxide reductase subunit F (NCBI ptt file), at 3,491,839 to 3,493,410
BT2811
BT2812: BT2812 - alkyl hydroperoxide reductase C22 protein (NCBI ptt file), at 3,493,475 to 3,494,041
BT2812
BT2813: BT2813 - sodium-dependent multivitamin transporter (NCBI ptt file), at 3,494,224 to 3,495,693
BT2813
BT2814: BT2814 - dihydrodipicolinate synthase (NCBI ptt file), at 3,495,718 to 3,496,647
BT2814
Position (kb)
3494
3495
3496 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3493.233 kb on + strand, within BT2811 at 3493.234 kb on - strand, within BT2811 at 3493.262 kb on - strand at 3493.320 kb on + strand at 3493.320 kb on + strand at 3493.321 kb on - strand at 3493.330 kb on - strand at 3493.330 kb on - strand at 3493.388 kb on - strand at 3493.463 kb on + strand at 3493.464 kb on - strand at 3493.474 kb on - strand at 3493.534 kb on + strand, within BT2812 at 3493.619 kb on - strand, within BT2812 at 3493.624 kb on + strand, within BT2812 at 3493.684 kb on - strand, within BT2812 at 3493.691 kb on - strand, within BT2812 at 3493.705 kb on + strand, within BT2812 at 3493.705 kb on + strand, within BT2812 at 3493.706 kb on - strand, within BT2812 at 3493.707 kb on + strand, within BT2812 at 3493.708 kb on - strand, within BT2812 at 3493.708 kb on - strand, within BT2812 at 3493.709 kb on + strand, within BT2812 at 3493.709 kb on + strand, within BT2812 at 3493.709 kb on + strand, within BT2812 at 3493.710 kb on - strand, within BT2812 at 3493.769 kb on - strand, within BT2812 at 3493.775 kb on - strand, within BT2812 at 3493.826 kb on + strand, within BT2812 at 3493.866 kb on + strand, within BT2812 at 3493.866 kb on + strand, within BT2812 at 3493.884 kb on - strand, within BT2812 at 3493.902 kb on - strand, within BT2812 at 3494.057 kb on + strand at 3494.080 kb on + strand at 3494.080 kb on + strand at 3494.082 kb on + strand at 3494.198 kb on - strand at 3494.440 kb on - strand, within BT2813 at 3494.441 kb on + strand, within BT2813 at 3494.566 kb on + strand, within BT2813 at 3494.567 kb on - strand, within BT2813 at 3494.579 kb on + strand, within BT2813 at 3494.580 kb on - strand, within BT2813 at 3494.580 kb on - strand, within BT2813 at 3494.621 kb on - strand, within BT2813 at 3494.625 kb on - strand, within BT2813 at 3494.636 kb on + strand, within BT2813 at 3494.655 kb on + strand, within BT2813 at 3494.677 kb on - strand, within BT2813 at 3494.685 kb on + strand, within BT2813 at 3494.686 kb on - strand, within BT2813 at 3494.750 kb on - strand, within BT2813 at 3494.759 kb on + strand, within BT2813 at 3494.816 kb on + strand, within BT2813 at 3494.817 kb on - strand, within BT2813 at 3494.825 kb on + strand, within BT2813 at 3494.825 kb on + strand, within BT2813 at 3494.826 kb on - strand, within BT2813 at 3494.826 kb on - strand, within BT2813 at 3494.826 kb on - strand, within BT2813 at 3494.839 kb on + strand, within BT2813 at 3494.840 kb on - strand, within BT2813 at 3494.840 kb on - strand, within BT2813 at 3494.840 kb on - strand, within BT2813 at 3494.840 kb on - strand, within BT2813 at 3494.901 kb on + strand, within BT2813 at 3494.942 kb on + strand, within BT2813 at 3494.943 kb on - strand, within BT2813 at 3494.955 kb on + strand, within BT2813 at 3494.969 kb on - strand, within BT2813 at 3494.977 kb on - strand, within BT2813 at 3494.981 kb on - strand, within BT2813 at 3494.992 kb on - strand, within BT2813 at 3495.061 kb on - strand, within BT2813 at 3495.244 kb on - strand, within BT2813 at 3495.244 kb on - strand, within BT2813 at 3495.271 kb on - strand, within BT2813 at 3495.288 kb on - strand, within BT2813 at 3495.295 kb on - strand, within BT2813 at 3495.343 kb on + strand, within BT2813 at 3495.344 kb on - strand, within BT2813 at 3495.344 kb on - strand, within BT2813 at 3495.344 kb on - strand, within BT2813 at 3495.383 kb on + strand, within BT2813 at 3495.410 kb on + strand, within BT2813 at 3495.411 kb on - strand, within BT2813 at 3495.425 kb on + strand, within BT2813 at 3495.425 kb on + strand, within BT2813 at 3495.426 kb on - strand, within BT2813 at 3495.429 kb on + strand, within BT2813 at 3495.492 kb on + strand, within BT2813 at 3495.493 kb on - strand, within BT2813 at 3495.493 kb on - strand, within BT2813 at 3495.494 kb on + strand, within BT2813 at 3495.495 kb on - strand, within BT2813 at 3495.518 kb on + strand, within BT2813 at 3495.533 kb on - strand, within BT2813 at 3495.537 kb on - strand, within BT2813 at 3495.537 kb on - strand, within BT2813 at 3495.537 kb on - strand, within BT2813 at 3495.587 kb on + strand at 3495.672 kb on + strand at 3495.673 kb on - strand at 3495.687 kb on + strand at 3495.693 kb on + strand at 3495.694 kb on - strand at 3495.793 kb on - strand at 3495.823 kb on + strand, within BT2814 at 3495.889 kb on - strand, within BT2814 at 3495.890 kb on + strand, within BT2814 at 3495.890 kb on + strand, within BT2814 at 3495.892 kb on + strand, within BT2814 at 3495.892 kb on + strand, within BT2814 at 3495.893 kb on - strand, within BT2814 at 3495.898 kb on - strand, within BT2814 at 3495.996 kb on + strand, within BT2814 at 3496.090 kb on - strand, within BT2814 at 3496.104 kb on - strand, within BT2814 at 3496.104 kb on - strand, within BT2814 at 3496.213 kb on - strand, within BT2814 at 3496.224 kb on + strand, within BT2814 at 3496.225 kb on - strand, within BT2814 at 3496.227 kb on + strand, within BT2814 at 3496.230 kb on - strand, within BT2814 at 3496.244 kb on - strand, within BT2814 at 3496.310 kb on + strand, within BT2814 at 3496.310 kb on + strand, within BT2814 at 3496.399 kb on - strand, within BT2814 at 3496.402 kb on + strand, within BT2814 at 3496.403 kb on - strand, within BT2814 at 3496.432 kb on - strand, within BT2814 at 3496.519 kb on + strand, within BT2814 at 3496.520 kb on - strand, within BT2814 at 3496.612 kb on - strand at 3496.612 kb on - strand at 3496.647 kb on + strand at 3496.682 kb on + strand at 3496.682 kb on + strand at 3496.682 kb on + strand at 3496.688 kb on + strand at 3496.688 kb on + strand at 3496.691 kb on + strand at 3496.692 kb on - strand at 3496.692 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 3,493,233 + BT2811 0.89 -2.3 3,493,234 - BT2811 0.89 -0.2 3,493,262 - -0.9 3,493,320 + +2.0 3,493,320 + -0.3 3,493,321 - +0.5 3,493,330 - -1.5 3,493,330 - -1.6 3,493,388 - -0.3 3,493,463 + -1.1 3,493,464 - -0.1 3,493,474 - -0.1 3,493,534 + BT2812 0.10 -0.1 3,493,619 - BT2812 0.25 -2.4 3,493,624 + BT2812 0.26 +1.1 3,493,684 - BT2812 0.37 -2.4 3,493,691 - BT2812 0.38 -1.5 3,493,705 + BT2812 0.41 -0.3 3,493,705 + BT2812 0.41 +0.5 3,493,706 - BT2812 0.41 -0.5 3,493,707 + BT2812 0.41 -2.5 3,493,708 - BT2812 0.41 -1.5 3,493,708 - BT2812 0.41 +1.7 3,493,709 + BT2812 0.41 +1.7 3,493,709 + BT2812 0.41 +0.6 3,493,709 + BT2812 0.41 -1.8 3,493,710 - BT2812 0.41 -1.4 3,493,769 - BT2812 0.52 -0.1 3,493,775 - BT2812 0.53 -0.9 3,493,826 + BT2812 0.62 +0.6 3,493,866 + BT2812 0.69 -0.1 3,493,866 + BT2812 0.69 -2.0 3,493,884 - BT2812 0.72 +1.0 3,493,902 - BT2812 0.75 +1.7 3,494,057 + -3.5 3,494,080 + -0.9 3,494,080 + +1.2 3,494,082 + -1.9 3,494,198 - +0.7 3,494,440 - BT2813 0.15 -1.5 3,494,441 + BT2813 0.15 -0.3 3,494,566 + BT2813 0.23 -1.7 3,494,567 - BT2813 0.23 +2.0 3,494,579 + BT2813 0.24 +0.5 3,494,580 - BT2813 0.24 +0.1 3,494,580 - BT2813 0.24 +1.7 3,494,621 - BT2813 0.27 -3.5 3,494,625 - BT2813 0.27 -3.5 3,494,636 + BT2813 0.28 +0.3 3,494,655 + BT2813 0.29 -0.5 3,494,677 - BT2813 0.31 -2.6 3,494,685 + BT2813 0.31 +0.2 3,494,686 - BT2813 0.31 -2.0 3,494,750 - BT2813 0.36 +0.1 3,494,759 + BT2813 0.36 +0.0 3,494,816 + BT2813 0.40 -0.6 3,494,817 - BT2813 0.40 +2.2 3,494,825 + BT2813 0.41 +0.7 3,494,825 + BT2813 0.41 -2.3 3,494,826 - BT2813 0.41 +1.2 3,494,826 - BT2813 0.41 -0.3 3,494,826 - BT2813 0.41 +0.1 3,494,839 + BT2813 0.42 -0.5 3,494,840 - BT2813 0.42 +0.1 3,494,840 - BT2813 0.42 +0.5 3,494,840 - BT2813 0.42 -0.3 3,494,840 - BT2813 0.42 -2.5 3,494,901 + BT2813 0.46 -0.6 3,494,942 + BT2813 0.49 -0.9 3,494,943 - BT2813 0.49 +1.4 3,494,955 + BT2813 0.50 -0.2 3,494,969 - BT2813 0.51 +0.4 3,494,977 - BT2813 0.51 -1.8 3,494,981 - BT2813 0.51 +0.5 3,494,992 - BT2813 0.52 -0.4 3,495,061 - BT2813 0.57 +0.5 3,495,244 - BT2813 0.69 -0.1 3,495,244 - BT2813 0.69 -2.7 3,495,271 - BT2813 0.71 +0.5 3,495,288 - BT2813 0.72 -0.9 3,495,295 - BT2813 0.73 -2.0 3,495,343 + BT2813 0.76 +1.7 3,495,344 - BT2813 0.76 -0.9 3,495,344 - BT2813 0.76 -1.7 3,495,344 - BT2813 0.76 +1.3 3,495,383 + BT2813 0.79 -1.3 3,495,410 + BT2813 0.81 +0.7 3,495,411 - BT2813 0.81 -2.0 3,495,425 + BT2813 0.82 +0.7 3,495,425 + BT2813 0.82 +2.3 3,495,426 - BT2813 0.82 -1.6 3,495,429 + BT2813 0.82 -1.9 3,495,492 + BT2813 0.86 -0.3 3,495,493 - BT2813 0.86 +0.5 3,495,493 - BT2813 0.86 -1.3 3,495,494 + BT2813 0.86 -2.5 3,495,495 - BT2813 0.86 +1.7 3,495,518 + BT2813 0.88 -1.9 3,495,533 - BT2813 0.89 -0.6 3,495,537 - BT2813 0.89 -3.5 3,495,537 - BT2813 0.89 -0.6 3,495,537 - BT2813 0.89 +0.6 3,495,587 + +2.0 3,495,672 + -0.6 3,495,673 - -1.4 3,495,687 + +1.7 3,495,693 + -0.5 3,495,694 - -2.8 3,495,793 - +1.3 3,495,823 + BT2814 0.11 -1.0 3,495,889 - BT2814 0.18 -0.1 3,495,890 + BT2814 0.18 +0.7 3,495,890 + BT2814 0.18 +0.7 3,495,892 + BT2814 0.19 -0.9 3,495,892 + BT2814 0.19 -2.1 3,495,893 - BT2814 0.19 -4.0 3,495,898 - BT2814 0.19 -2.1 3,495,996 + BT2814 0.30 -1.1 3,496,090 - BT2814 0.40 -0.5 3,496,104 - BT2814 0.42 +0.9 3,496,104 - BT2814 0.42 +1.8 3,496,213 - BT2814 0.53 -3.1 3,496,224 + BT2814 0.54 -0.2 3,496,225 - BT2814 0.55 -2.4 3,496,227 + BT2814 0.55 -0.2 3,496,230 - BT2814 0.55 +0.6 3,496,244 - BT2814 0.57 -0.6 3,496,310 + BT2814 0.64 -0.3 3,496,310 + BT2814 0.64 +2.1 3,496,399 - BT2814 0.73 -2.6 3,496,402 + BT2814 0.74 -3.7 3,496,403 - BT2814 0.74 -2.0 3,496,432 - BT2814 0.77 -1.3 3,496,519 + BT2814 0.86 -0.4 3,496,520 - BT2814 0.86 -0.3 3,496,612 - -1.1 3,496,612 - -0.9 3,496,647 + -2.9 3,496,682 + +1.7 3,496,682 + +0.6 3,496,682 + -2.2 3,496,688 + -1.1 3,496,688 + -3.6 3,496,691 + +1.4 3,496,692 - -1.7 3,496,692 - -0.1
Or see this region's nucleotide sequence