Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2359 and BT2360 are separated by 711 nucleotides BT2360 and BT2361 are separated by 43 nucleotides BT2361 and BT2362 are separated by 331 nucleotides
BT2359: BT2359 - formamidopyrimidine-DNA glycosylase (NCBI ptt file), at 2,939,709 to 2,940,530
BT2359
BT2360: BT2360 - transcriptional regulator (NCBI ptt file), at 2,941,242 to 2,941,652
BT2360
BT2361: BT2361 - hypothetical protein (NCBI ptt file), at 2,941,696 to 2,942,085
BT2361
BT2362: BT2362 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,942,417 to 2,945,503
BT2362
Position (kb)
2941
2942 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2940.253 kb on + strand, within BT2359 at 2940.305 kb on + strand, within BT2359 at 2940.361 kb on + strand, within BT2359 at 2940.362 kb on - strand, within BT2359 at 2940.378 kb on - strand, within BT2359 at 2940.389 kb on + strand, within BT2359 at 2940.391 kb on + strand, within BT2359 at 2940.504 kb on + strand at 2940.505 kb on - strand at 2940.505 kb on - strand at 2940.516 kb on + strand at 2940.517 kb on - strand at 2940.534 kb on + strand at 2940.534 kb on + strand at 2940.535 kb on - strand at 2940.539 kb on + strand at 2940.616 kb on + strand at 2940.616 kb on + strand at 2940.636 kb on + strand at 2940.685 kb on - strand at 2940.689 kb on - strand at 2940.693 kb on + strand at 2940.736 kb on - strand at 2940.751 kb on - strand at 2940.789 kb on + strand at 2940.832 kb on - strand at 2940.889 kb on - strand at 2940.973 kb on - strand at 2941.008 kb on + strand at 2941.036 kb on + strand at 2941.058 kb on + strand at 2941.080 kb on - strand at 2941.099 kb on - strand at 2941.125 kb on - strand at 2941.125 kb on - strand at 2941.131 kb on + strand at 2941.131 kb on + strand at 2941.135 kb on + strand at 2941.135 kb on + strand at 2941.136 kb on - strand at 2941.193 kb on + strand at 2941.194 kb on - strand at 2941.200 kb on + strand at 2941.201 kb on - strand at 2941.240 kb on + strand at 2941.244 kb on - strand at 2941.244 kb on - strand at 2941.248 kb on - strand at 2941.273 kb on + strand at 2941.274 kb on - strand at 2941.274 kb on - strand at 2941.278 kb on - strand at 2941.293 kb on - strand, within BT2360 at 2941.309 kb on - strand, within BT2360 at 2941.362 kb on - strand, within BT2360 at 2941.368 kb on + strand, within BT2360 at 2941.368 kb on + strand, within BT2360 at 2941.369 kb on - strand, within BT2360 at 2941.371 kb on - strand, within BT2360 at 2941.371 kb on - strand, within BT2360 at 2941.383 kb on + strand, within BT2360 at 2941.415 kb on - strand, within BT2360 at 2941.417 kb on + strand, within BT2360 at 2941.418 kb on - strand, within BT2360 at 2941.418 kb on - strand, within BT2360 at 2941.418 kb on - strand, within BT2360 at 2941.418 kb on - strand, within BT2360 at 2941.447 kb on - strand, within BT2360 at 2941.488 kb on + strand, within BT2360 at 2941.532 kb on + strand, within BT2360 at 2941.596 kb on + strand, within BT2360 at 2941.596 kb on + strand, within BT2360 at 2941.606 kb on - strand at 2941.688 kb on - strand at 2941.822 kb on - strand, within BT2361 at 2941.822 kb on - strand, within BT2361 at 2941.844 kb on + strand, within BT2361 at 2941.844 kb on + strand, within BT2361 at 2941.845 kb on - strand, within BT2361 at 2941.845 kb on - strand, within BT2361 at 2941.845 kb on - strand, within BT2361 at 2941.845 kb on - strand, within BT2361 at 2941.847 kb on - strand, within BT2361 at 2941.888 kb on + strand, within BT2361 at 2942.016 kb on - strand, within BT2361 at 2942.038 kb on - strand, within BT2361 at 2942.038 kb on - strand, within BT2361 at 2942.085 kb on + strand at 2942.086 kb on - strand at 2942.098 kb on + strand at 2942.107 kb on - strand at 2942.185 kb on - strand at 2942.249 kb on + strand at 2942.250 kb on - strand at 2942.301 kb on - strand at 2942.335 kb on + strand at 2942.445 kb on + strand at 2942.445 kb on + strand at 2942.453 kb on + strand at 2942.454 kb on - strand at 2942.454 kb on - strand at 2942.454 kb on - strand at 2942.459 kb on - strand at 2942.475 kb on - strand at 2942.481 kb on + strand at 2942.482 kb on - strand at 2942.544 kb on - strand at 2942.611 kb on + strand at 2942.614 kb on + strand at 2942.615 kb on - strand at 2942.647 kb on + strand at 2942.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 2,940,253 + BT2359 0.66 +2.0 2,940,305 + BT2359 0.73 +0.3 2,940,361 + BT2359 0.79 +0.9 2,940,362 - BT2359 0.79 -0.3 2,940,378 - BT2359 0.81 +0.8 2,940,389 + BT2359 0.83 -0.8 2,940,391 + BT2359 0.83 +2.5 2,940,504 + -2.9 2,940,505 - +2.8 2,940,505 - -1.6 2,940,516 + +1.5 2,940,517 - -1.2 2,940,534 + -3.0 2,940,534 + -0.8 2,940,535 - -1.8 2,940,539 + -0.7 2,940,616 + +0.5 2,940,616 + -1.5 2,940,636 + -1.8 2,940,685 - -1.5 2,940,689 - -1.9 2,940,693 + +0.5 2,940,736 - -2.6 2,940,751 - +0.3 2,940,789 + +0.9 2,940,832 - -0.2 2,940,889 - -2.6 2,940,973 - -1.4 2,941,008 + -0.5 2,941,036 + +1.5 2,941,058 + -3.5 2,941,080 - -3.5 2,941,099 - -2.3 2,941,125 - -0.2 2,941,125 - +0.2 2,941,131 + +3.5 2,941,131 + -1.4 2,941,135 + +0.2 2,941,135 + -2.7 2,941,136 - +0.2 2,941,193 + +0.2 2,941,194 - -1.8 2,941,200 + -1.1 2,941,201 - -3.6 2,941,240 + -0.3 2,941,244 - +0.2 2,941,244 - +2.9 2,941,248 - -3.1 2,941,273 + -1.6 2,941,274 - +0.8 2,941,274 - -3.0 2,941,278 - +1.5 2,941,293 - BT2360 0.12 -0.5 2,941,309 - BT2360 0.16 -2.2 2,941,362 - BT2360 0.29 -2.1 2,941,368 + BT2360 0.31 -0.4 2,941,368 + BT2360 0.31 -0.1 2,941,369 - BT2360 0.31 -2.3 2,941,371 - BT2360 0.31 -2.8 2,941,371 - BT2360 0.31 -1.3 2,941,383 + BT2360 0.34 -3.0 2,941,415 - BT2360 0.42 +0.2 2,941,417 + BT2360 0.43 +0.4 2,941,418 - BT2360 0.43 -3.0 2,941,418 - BT2360 0.43 -3.2 2,941,418 - BT2360 0.43 -0.1 2,941,418 - BT2360 0.43 +1.5 2,941,447 - BT2360 0.50 +0.3 2,941,488 + BT2360 0.60 -2.3 2,941,532 + BT2360 0.71 -2.7 2,941,596 + BT2360 0.86 -0.2 2,941,596 + BT2360 0.86 -1.9 2,941,606 - +1.5 2,941,688 - +0.5 2,941,822 - BT2361 0.32 -2.2 2,941,822 - BT2361 0.32 -2.7 2,941,844 + BT2361 0.38 -0.8 2,941,844 + BT2361 0.38 -3.0 2,941,845 - BT2361 0.38 -0.5 2,941,845 - BT2361 0.38 -1.2 2,941,845 - BT2361 0.38 -1.3 2,941,845 - BT2361 0.38 -0.7 2,941,847 - BT2361 0.39 -0.1 2,941,888 + BT2361 0.49 +0.5 2,942,016 - BT2361 0.82 +0.5 2,942,038 - BT2361 0.88 -2.5 2,942,038 - BT2361 0.88 -0.3 2,942,085 + -0.8 2,942,086 - -1.5 2,942,098 + -1.2 2,942,107 - -2.8 2,942,185 - -0.9 2,942,249 + +0.2 2,942,250 - -2.6 2,942,301 - +0.9 2,942,335 + -1.1 2,942,445 + -2.0 2,942,445 + +0.5 2,942,453 + -1.9 2,942,454 - -0.9 2,942,454 - -0.3 2,942,454 - -0.8 2,942,459 - -0.1 2,942,475 - -2.4 2,942,481 + -0.8 2,942,482 - -0.9 2,942,544 - +1.6 2,942,611 + -0.4 2,942,614 + -0.1 2,942,615 - -0.1 2,942,647 + -1.9 2,942,647 + -1.6
Or see this region's nucleotide sequence