Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1321 and BT1322 are separated by 7 nucleotides BT1322 and BT1323 are separated by 1 nucleotides BT1323 and BT1324 are separated by 176 nucleotides
BT1321: BT1321 - putative phosphate transport ATP-binding protein (NCBI ptt file), at 1,646,268 to 1,647,026
BT1321
BT1322: BT1322 - putative ABC transporter permease protein (NCBI ptt file), at 1,647,034 to 1,647,909
BT1322
BT1323: BT1323 - putative ABC transporter permease protein (NCBI ptt file), at 1,647,911 to 1,649,107
BT1323
BT1324: BT1324 - phosphate ABC transporter, phosphate-binding protein (NCBI ptt file), at 1,649,284 to 1,650,096
BT1324
Position (kb)
1647
1648
1649
1650 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1646.984 kb on - strand at 1647.026 kb on + strand at 1647.131 kb on + strand, within BT1322 at 1647.132 kb on - strand, within BT1322 at 1647.132 kb on - strand, within BT1322 at 1647.142 kb on + strand, within BT1322 at 1647.142 kb on + strand, within BT1322 at 1647.183 kb on - strand, within BT1322 at 1647.204 kb on + strand, within BT1322 at 1647.245 kb on + strand, within BT1322 at 1647.307 kb on + strand, within BT1322 at 1647.790 kb on + strand, within BT1322 at 1647.792 kb on + strand, within BT1322 at 1647.829 kb on - strand at 1647.894 kb on + strand at 1647.920 kb on - strand at 1647.945 kb on + strand at 1647.945 kb on + strand at 1647.968 kb on + strand at 1647.968 kb on + strand at 1647.986 kb on + strand at 1648.210 kb on + strand, within BT1323 at 1648.216 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.218 kb on + strand, within BT1323 at 1648.220 kb on + strand, within BT1323 at 1648.315 kb on + strand, within BT1323 at 1648.342 kb on + strand, within BT1323 at 1648.444 kb on + strand, within BT1323 at 1648.517 kb on - strand, within BT1323 at 1648.618 kb on + strand, within BT1323 at 1648.667 kb on - strand, within BT1323 at 1648.669 kb on + strand, within BT1323 at 1648.669 kb on + strand, within BT1323 at 1648.708 kb on + strand, within BT1323 at 1648.779 kb on + strand, within BT1323 at 1648.810 kb on + strand, within BT1323 at 1648.812 kb on + strand, within BT1323 at 1648.910 kb on + strand, within BT1323 at 1648.966 kb on - strand, within BT1323 at 1648.987 kb on + strand, within BT1323 at 1649.041 kb on + strand at 1649.041 kb on + strand at 1649.044 kb on + strand at 1649.044 kb on + strand at 1649.081 kb on + strand at 1649.107 kb on + strand at 1649.107 kb on + strand at 1649.108 kb on - strand at 1649.111 kb on + strand at 1649.574 kb on - strand, within BT1324 at 1649.705 kb on + strand, within BT1324 at 1649.737 kb on - strand, within BT1324 at 1649.876 kb on + strand, within BT1324 at 1649.983 kb on + strand, within BT1324 at 1649.984 kb on - strand, within BT1324 at 1649.984 kb on - strand, within BT1324 at 1649.986 kb on + strand, within BT1324 at 1649.989 kb on + strand, within BT1324 at 1649.992 kb on + strand, within BT1324 at 1649.992 kb on + strand, within BT1324 at 1649.993 kb on - strand, within BT1324 at 1649.995 kb on - strand, within BT1324 at 1650.064 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 1,646,984 - +0.2 1,647,026 + -1.0 1,647,131 + BT1322 0.11 -1.5 1,647,132 - BT1322 0.11 -2.6 1,647,132 - BT1322 0.11 -1.3 1,647,142 + BT1322 0.12 -0.9 1,647,142 + BT1322 0.12 -2.5 1,647,183 - BT1322 0.17 -0.4 1,647,204 + BT1322 0.19 -0.1 1,647,245 + BT1322 0.24 +1.5 1,647,307 + BT1322 0.31 -2.8 1,647,790 + BT1322 0.86 -0.6 1,647,792 + BT1322 0.87 -3.9 1,647,829 - -2.3 1,647,894 + +0.5 1,647,920 - -2.7 1,647,945 + -0.7 1,647,945 + +0.6 1,647,968 + +0.1 1,647,968 + +0.1 1,647,986 + +0.8 1,648,210 + BT1323 0.25 -1.4 1,648,216 + BT1323 0.25 +0.5 1,648,218 + BT1323 0.26 -0.9 1,648,218 + BT1323 0.26 -3.1 1,648,218 + BT1323 0.26 +1.5 1,648,220 + BT1323 0.26 +1.3 1,648,315 + BT1323 0.34 -3.4 1,648,342 + BT1323 0.36 -2.2 1,648,444 + BT1323 0.45 -1.4 1,648,517 - BT1323 0.51 -2.5 1,648,618 + BT1323 0.59 -0.1 1,648,667 - BT1323 0.63 -2.9 1,648,669 + BT1323 0.63 -0.5 1,648,669 + BT1323 0.63 -1.5 1,648,708 + BT1323 0.67 +1.0 1,648,779 + BT1323 0.73 +0.2 1,648,810 + BT1323 0.75 -0.5 1,648,812 + BT1323 0.75 -4.0 1,648,910 + BT1323 0.83 -2.2 1,648,966 - BT1323 0.88 -2.7 1,648,987 + BT1323 0.90 -2.1 1,649,041 + +0.8 1,649,041 + -1.4 1,649,044 + -1.2 1,649,044 + -2.2 1,649,081 + -0.9 1,649,107 + -1.1 1,649,107 + -1.5 1,649,108 - -0.5 1,649,111 + -2.2 1,649,574 - BT1324 0.36 +2.1 1,649,705 + BT1324 0.52 -2.0 1,649,737 - BT1324 0.56 -1.0 1,649,876 + BT1324 0.73 -0.5 1,649,983 + BT1324 0.86 -1.1 1,649,984 - BT1324 0.86 -0.4 1,649,984 - BT1324 0.86 +0.2 1,649,986 + BT1324 0.86 -0.1 1,649,989 + BT1324 0.87 -1.0 1,649,992 + BT1324 0.87 +1.7 1,649,992 + BT1324 0.87 -0.1 1,649,993 - BT1324 0.87 +0.1 1,649,995 - BT1324 0.87 +2.1 1,650,064 - -1.4
Or see this region's nucleotide sequence