Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0325 and BT0326 overlap by 4 nucleotides BT0326 and BT0327 are separated by 442 nucleotides BT0327 and BT0328 are separated by 17 nucleotides
BT0325: BT0325 - hypothetical protein (NCBI ptt file), at 391,028 to 391,489
BT0325
BT0326: BT0326 - putative RNA polymerase ECF-type sigma factor (NCBI ptt file), at 391,486 to 392,007
BT0326
BT0327: BT0327 - glutamine ABC transporter, periplasmic glutamine-binding protein (glnH) (NCBI ptt file), at 392,450 to 393,289
BT0327
BT0328: BT0328 - hypothetical protein (NCBI ptt file), at 393,307 to 395,349
BT0328
Position (kb) 392
393
394 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 391.800 kb on + strand, within BT0326 at 391.984 kb on + strand at 392.168 kb on + strand at 392.196 kb on + strand at 392.219 kb on - strand at 392.224 kb on - strand at 392.231 kb on + strand at 392.232 kb on - strand at 392.235 kb on + strand at 392.363 kb on - strand at 392.363 kb on - strand at 392.363 kb on - strand at 392.403 kb on - strand at 392.464 kb on - strand at 392.464 kb on - strand at 392.522 kb on - strand at 392.603 kb on - strand, within BT0327 at 392.734 kb on + strand, within BT0327 at 392.735 kb on - strand, within BT0327 at 392.894 kb on + strand, within BT0327 at 393.070 kb on - strand, within BT0327 at 393.118 kb on + strand, within BT0327 at 393.121 kb on - strand, within BT0327 at 393.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 391,800 + BT0326 0.60 +0.7 391,984 + -0.3 392,168 + -1.9 392,196 + -1.3 392,219 - -0.1 392,224 - -0.6 392,231 + -2.5 392,232 - -1.0 392,235 + +0.3 392,363 - +0.9 392,363 - +0.5 392,363 - -1.6 392,403 - +1.6 392,464 - -0.3 392,464 - -2.0 392,522 - +1.6 392,603 - BT0327 0.18 -2.5 392,734 + BT0327 0.34 -1.0 392,735 - BT0327 0.34 -3.1 392,894 + BT0327 0.53 -3.0 393,070 - BT0327 0.74 -1.4 393,118 + BT0327 0.80 -4.1 393,121 - BT0327 0.80 -0.4 393,234 - +0.6
Or see this region's nucleotide sequence