Experiment: BHIS with Dimethyl Sulfoxide 9 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0177 and BT0178 are separated by 26 nucleotides BT0178 and BT0179 are separated by 9 nucleotides BT0179 and BT0180 are separated by 89 nucleotides
BT0177: BT0177 - hypothetical protein (NCBI ptt file), at 185,638 to 186,429
BT0177
BT0178: BT0178 - hypothetical protein (NCBI ptt file), at 186,456 to 187,001
BT0178
BT0179: BT0179 - SsrA-binding protein (NCBI ptt file), at 187,011 to 187,463
BT0179
BT0180: BT0180 - 5-methyltetrahydrofolate-homocysteine methyltransferase (NCBI ptt file), at 187,553 to 190,300
BT0180
Position (kb) 187
188 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 186.016 kb on + strand, within BT0177 at 186.033 kb on + strand, within BT0177 at 186.088 kb on + strand, within BT0177 at 186.108 kb on + strand, within BT0177 at 186.160 kb on + strand, within BT0177 at 186.160 kb on + strand, within BT0177 at 186.318 kb on + strand, within BT0177 at 186.449 kb on + strand at 186.451 kb on + strand at 186.545 kb on + strand, within BT0178 at 186.625 kb on + strand, within BT0178 at 186.695 kb on + strand, within BT0178 at 186.855 kb on + strand, within BT0178 at 186.943 kb on + strand, within BT0178 at 186.946 kb on + strand, within BT0178 at 186.946 kb on + strand, within BT0178 at 186.991 kb on + strand at 186.999 kb on + strand at 187.480 kb on - strand at 187.502 kb on - strand at 187.512 kb on + strand at 187.540 kb on + strand at 187.541 kb on - strand at 187.632 kb on - strand at 187.632 kb on - strand at 187.676 kb on - strand at 187.742 kb on - strand at 187.789 kb on + strand at 187.838 kb on + strand, within BT0180 at 187.895 kb on + strand, within BT0180 at 187.948 kb on + strand, within BT0180 at 187.949 kb on - strand, within BT0180 at 188.141 kb on - strand, within BT0180 at 188.157 kb on + strand, within BT0180 at 188.157 kb on + strand, within BT0180 at 188.158 kb on - strand, within BT0180 at 188.207 kb on - strand, within BT0180 at 188.207 kb on - strand, within BT0180 at 188.261 kb on - strand, within BT0180 at 188.345 kb on + strand, within BT0180 at 188.362 kb on + strand, within BT0180 at 188.363 kb on - strand, within BT0180 at 188.363 kb on - strand, within BT0180 at 188.438 kb on - strand, within BT0180
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimethyl Sulfoxide 9 vol% remove 186,016 + BT0177 0.48 +3.1 186,033 + BT0177 0.50 +1.5 186,088 + BT0177 0.57 +1.5 186,108 + BT0177 0.59 -3.2 186,160 + BT0177 0.66 +0.5 186,160 + BT0177 0.66 -0.2 186,318 + BT0177 0.86 +0.1 186,449 + -1.3 186,451 + -0.5 186,545 + BT0178 0.16 -3.4 186,625 + BT0178 0.31 -1.4 186,695 + BT0178 0.44 -1.9 186,855 + BT0178 0.73 -3.1 186,943 + BT0178 0.89 -3.9 186,946 + BT0178 0.90 -1.7 186,946 + BT0178 0.90 -2.7 186,991 + -1.4 186,999 + +1.2 187,480 - -0.1 187,502 - -1.7 187,512 + -1.4 187,540 + +0.9 187,541 - +0.2 187,632 - -3.2 187,632 - -0.2 187,676 - +1.1 187,742 - +1.2 187,789 + -1.9 187,838 + BT0180 0.10 -2.3 187,895 + BT0180 0.12 -1.3 187,948 + BT0180 0.14 +0.2 187,949 - BT0180 0.14 +0.1 188,141 - BT0180 0.21 -0.3 188,157 + BT0180 0.22 +0.5 188,157 + BT0180 0.22 +0.9 188,158 - BT0180 0.22 -0.2 188,207 - BT0180 0.24 -0.1 188,207 - BT0180 0.24 +0.9 188,261 - BT0180 0.26 +0.6 188,345 + BT0180 0.29 -1.1 188,362 + BT0180 0.29 -1.2 188,363 - BT0180 0.29 -0.5 188,363 - BT0180 0.29 -1.2 188,438 - BT0180 0.32 +1.5
Or see this region's nucleotide sequence