Experiment: m.b. nitrite 8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05650 and PGA1_c05660 are separated by 145 nucleotides PGA1_c05660 and PGA1_c05670 overlap by 4 nucleotides PGA1_c05670 and PGA1_c05680 overlap by 4 nucleotides
PGA1_c05650: PGA1_c05650 - tRNA pseudouridine synthase A, at 577,398 to 578,171
_c05650
PGA1_c05660: PGA1_c05660 - Predicted ATPase, at 578,317 to 579,732
_c05660
PGA1_c05670: PGA1_c05670 - acetyltransferase domain-containing protein, at 579,729 to 580,283
_c05670
PGA1_c05680: PGA1_c05680 - Predicted membrane protein, at 580,280 to 581,335
_c05680
Position (kb)
578
579
580 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 577.349 kb on + strand at 577.357 kb on - strand at 577.357 kb on - strand at 577.357 kb on - strand at 577.359 kb on + strand at 577.367 kb on - strand at 577.374 kb on + strand at 577.467 kb on - strand at 577.544 kb on + strand, within PGA1_c05650 at 577.571 kb on - strand, within PGA1_c05650 at 577.637 kb on - strand, within PGA1_c05650 at 578.124 kb on + strand at 578.251 kb on + strand at 578.251 kb on + strand at 578.251 kb on + strand at 578.251 kb on + strand at 578.259 kb on - strand at 578.259 kb on - strand at 578.259 kb on - strand at 578.259 kb on - strand at 578.259 kb on - strand at 578.259 kb on - strand at 578.291 kb on - strand at 578.344 kb on + strand at 578.352 kb on - strand at 578.354 kb on + strand at 578.354 kb on + strand at 578.354 kb on + strand at 578.354 kb on + strand at 578.430 kb on - strand at 578.430 kb on - strand at 578.430 kb on - strand at 578.456 kb on + strand at 578.456 kb on + strand at 578.489 kb on + strand, within PGA1_c05660 at 578.647 kb on + strand, within PGA1_c05660 at 578.667 kb on + strand, within PGA1_c05660 at 578.668 kb on - strand, within PGA1_c05660 at 578.782 kb on + strand, within PGA1_c05660 at 578.947 kb on + strand, within PGA1_c05660 at 578.952 kb on + strand, within PGA1_c05660 at 578.952 kb on + strand, within PGA1_c05660 at 579.010 kb on - strand, within PGA1_c05660 at 579.014 kb on + strand, within PGA1_c05660 at 579.159 kb on + strand, within PGA1_c05660 at 579.213 kb on - strand, within PGA1_c05660 at 579.323 kb on - strand, within PGA1_c05660 at 579.419 kb on + strand, within PGA1_c05660 at 579.490 kb on - strand, within PGA1_c05660 at 579.495 kb on - strand, within PGA1_c05660 at 579.511 kb on + strand, within PGA1_c05660 at 579.511 kb on + strand, within PGA1_c05660 at 579.511 kb on + strand, within PGA1_c05660 at 579.519 kb on - strand, within PGA1_c05660 at 579.553 kb on + strand, within PGA1_c05660 at 579.629 kb on - strand at 579.649 kb on + strand at 579.649 kb on + strand at 579.657 kb on - strand at 579.657 kb on - strand at 579.679 kb on + strand at 579.679 kb on + strand at 579.687 kb on - strand at 579.688 kb on + strand at 579.733 kb on - strand at 579.735 kb on + strand at 579.744 kb on - strand at 579.777 kb on - strand at 579.842 kb on + strand, within PGA1_c05670 at 579.852 kb on + strand, within PGA1_c05670 at 579.898 kb on + strand, within PGA1_c05670 at 579.901 kb on - strand, within PGA1_c05670 at 580.046 kb on + strand, within PGA1_c05670 at 580.054 kb on - strand, within PGA1_c05670 at 580.054 kb on - strand, within PGA1_c05670 at 580.100 kb on - strand, within PGA1_c05670 at 580.112 kb on + strand, within PGA1_c05670 at 580.112 kb on + strand, within PGA1_c05670 at 580.137 kb on + strand, within PGA1_c05670 at 580.145 kb on - strand, within PGA1_c05670 at 580.239 kb on - strand at 580.239 kb on - strand at 580.284 kb on + strand at 580.370 kb on + strand at 580.429 kb on + strand, within PGA1_c05680 at 580.494 kb on - strand, within PGA1_c05680 at 580.574 kb on + strand, within PGA1_c05680
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 8 mM remove 577,349 + -0.5 577,357 - -0.9 577,357 - +0.2 577,357 - +1.2 577,359 + +1.8 577,367 - +3.9 577,374 + -1.4 577,467 - +0.3 577,544 + PGA1_c05650 0.19 -1.2 577,571 - PGA1_c05650 0.22 +0.9 577,637 - PGA1_c05650 0.31 -0.2 578,124 + -1.5 578,251 + -0.9 578,251 + -0.7 578,251 + +2.2 578,251 + -1.4 578,259 - -2.3 578,259 - +2.3 578,259 - -1.4 578,259 - +0.6 578,259 - -0.9 578,259 - -0.9 578,291 - -1.9 578,344 + +0.2 578,352 - +0.9 578,354 + -1.9 578,354 + +0.6 578,354 + -0.2 578,354 + -1.9 578,430 - -0.5 578,430 - +0.3 578,430 - -0.8 578,456 + +0.5 578,456 + +0.2 578,489 + PGA1_c05660 0.12 -0.4 578,647 + PGA1_c05660 0.23 +2.2 578,667 + PGA1_c05660 0.25 +0.1 578,668 - PGA1_c05660 0.25 -1.9 578,782 + PGA1_c05660 0.33 +2.0 578,947 + PGA1_c05660 0.44 +1.1 578,952 + PGA1_c05660 0.45 -1.9 578,952 + PGA1_c05660 0.45 +0.5 579,010 - PGA1_c05660 0.49 +2.2 579,014 + PGA1_c05660 0.49 +1.5 579,159 + PGA1_c05660 0.59 +2.7 579,213 - PGA1_c05660 0.63 -0.7 579,323 - PGA1_c05660 0.71 +0.8 579,419 + PGA1_c05660 0.78 -0.5 579,490 - PGA1_c05660 0.83 +0.6 579,495 - PGA1_c05660 0.83 +0.9 579,511 + PGA1_c05660 0.84 -0.2 579,511 + PGA1_c05660 0.84 -0.4 579,511 + PGA1_c05660 0.84 +1.6 579,519 - PGA1_c05660 0.85 -0.9 579,553 + PGA1_c05660 0.87 -1.5 579,629 - -1.4 579,649 + +4.0 579,649 + +1.2 579,657 - +0.2 579,657 - +1.6 579,679 + +3.4 579,679 + +2.3 579,687 - -2.1 579,688 + +2.1 579,733 - +0.5 579,735 + -0.5 579,744 - +1.6 579,777 - +1.1 579,842 + PGA1_c05670 0.20 +3.0 579,852 + PGA1_c05670 0.22 +0.1 579,898 + PGA1_c05670 0.30 +1.1 579,901 - PGA1_c05670 0.31 +0.4 580,046 + PGA1_c05670 0.57 +2.5 580,054 - PGA1_c05670 0.59 +0.1 580,054 - PGA1_c05670 0.59 +2.4 580,100 - PGA1_c05670 0.67 +0.5 580,112 + PGA1_c05670 0.69 +0.1 580,112 + PGA1_c05670 0.69 +1.6 580,137 + PGA1_c05670 0.74 +0.3 580,145 - PGA1_c05670 0.75 +1.1 580,239 - -1.2 580,239 - +4.5 580,284 + +0.6 580,370 + -0.1 580,429 + PGA1_c05680 0.14 -0.1 580,494 - PGA1_c05680 0.20 +3.1 580,574 + PGA1_c05680 0.28 -1.5
Or see this region's nucleotide sequence