Experiment: m.b. nitrite 8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c22230 and PGA1_c22240 are separated by 506 nucleotides PGA1_c22240 and PGA1_c22250 overlap by 4 nucleotides PGA1_c22250 and PGA1_c22260 are separated by 189 nucleotides PGA1_c22260 and PGA1_c22270 are separated by 9 nucleotides PGA1_c22270 and PGA1_c22280 overlap by 20 nucleotides
PGA1_c22230: PGA1_c22230 - hypothetical protein, at 2,307,308 to 2,311,108
_c22230
PGA1_c22240: PGA1_c22240 - hypothetical protein, at 2,311,615 to 2,311,917
_c22240
PGA1_c22250: PGA1_c22250 - hypothetical protein, at 2,311,914 to 2,312,579
_c22250
PGA1_c22260: PGA1_c22260 - hypothetical protein, at 2,312,769 to 2,313,146
_c22260
PGA1_c22270: PGA1_c22270 - Protein of unknown function (DUF1353)., at 2,313,156 to 2,313,500
_c22270
PGA1_c22280: PGA1_c22280 - hypothetical protein, at 2,313,481 to 2,313,954
_c22280
Position (kb)
2311
2312
2313 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2310.949 kb on - strand at 2310.976 kb on + strand at 2310.984 kb on - strand at 2311.026 kb on - strand at 2311.054 kb on - strand at 2311.058 kb on - strand at 2311.077 kb on + strand at 2311.089 kb on - strand at 2311.120 kb on - strand at 2311.146 kb on + strand at 2311.146 kb on + strand at 2311.146 kb on + strand at 2311.183 kb on + strand at 2311.183 kb on + strand at 2311.190 kb on + strand at 2311.191 kb on - strand at 2311.248 kb on + strand at 2311.353 kb on + strand at 2311.365 kb on - strand at 2311.477 kb on + strand at 2311.477 kb on + strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.485 kb on - strand at 2311.488 kb on + strand at 2311.494 kb on + strand at 2311.497 kb on - strand at 2311.502 kb on - strand at 2311.528 kb on + strand at 2311.582 kb on + strand at 2311.590 kb on - strand at 2311.590 kb on - strand at 2311.590 kb on - strand at 2311.657 kb on - strand, within PGA1_c22240 at 2311.695 kb on - strand, within PGA1_c22240 at 2311.749 kb on + strand, within PGA1_c22240 at 2311.777 kb on - strand, within PGA1_c22240 at 2311.836 kb on - strand, within PGA1_c22240 at 2311.838 kb on - strand, within PGA1_c22240 at 2312.082 kb on + strand, within PGA1_c22250 at 2312.082 kb on + strand, within PGA1_c22250 at 2312.118 kb on + strand, within PGA1_c22250 at 2312.149 kb on + strand, within PGA1_c22250 at 2312.153 kb on + strand, within PGA1_c22250 at 2312.287 kb on + strand, within PGA1_c22250 at 2312.311 kb on + strand, within PGA1_c22250 at 2312.327 kb on + strand, within PGA1_c22250 at 2312.400 kb on - strand, within PGA1_c22250 at 2312.405 kb on - strand, within PGA1_c22250 at 2312.438 kb on - strand, within PGA1_c22250 at 2312.462 kb on + strand, within PGA1_c22250 at 2312.540 kb on - strand at 2312.540 kb on - strand at 2312.540 kb on - strand at 2312.566 kb on - strand at 2312.629 kb on + strand at 2312.632 kb on - strand at 2312.637 kb on - strand at 2312.773 kb on - strand at 2312.778 kb on - strand at 2312.839 kb on - strand, within PGA1_c22260 at 2312.865 kb on - strand, within PGA1_c22260 at 2312.886 kb on + strand, within PGA1_c22260 at 2312.930 kb on - strand, within PGA1_c22260 at 2312.949 kb on - strand, within PGA1_c22260 at 2312.956 kb on + strand, within PGA1_c22260 at 2312.974 kb on - strand, within PGA1_c22260 at 2312.974 kb on - strand, within PGA1_c22260 at 2312.984 kb on + strand, within PGA1_c22260 at 2313.094 kb on - strand, within PGA1_c22260 at 2313.154 kb on + strand at 2313.223 kb on + strand, within PGA1_c22270 at 2313.258 kb on + strand, within PGA1_c22270 at 2313.258 kb on + strand, within PGA1_c22270 at 2313.261 kb on - strand, within PGA1_c22270 at 2313.263 kb on + strand, within PGA1_c22270 at 2313.291 kb on + strand, within PGA1_c22270 at 2313.291 kb on + strand, within PGA1_c22270 at 2313.418 kb on + strand, within PGA1_c22270 at 2313.418 kb on + strand, within PGA1_c22270 at 2313.421 kb on - strand, within PGA1_c22270 at 2313.450 kb on + strand, within PGA1_c22270 at 2313.464 kb on - strand, within PGA1_c22270 at 2313.486 kb on + strand at 2313.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 8 mM remove 2,310,949 - +0.9 2,310,976 + +0.4 2,310,984 - -2.4 2,311,026 - -2.8 2,311,054 - -1.4 2,311,058 - -3.6 2,311,077 + +0.2 2,311,089 - +1.2 2,311,120 - +0.6 2,311,146 + +0.9 2,311,146 + -3.4 2,311,146 + -1.8 2,311,183 + -1.4 2,311,183 + +0.3 2,311,190 + -1.3 2,311,191 - -2.3 2,311,248 + -1.2 2,311,353 + -1.0 2,311,365 - +1.6 2,311,477 + -1.0 2,311,477 + -0.2 2,311,485 - -0.1 2,311,485 - -2.0 2,311,485 - -1.4 2,311,485 - -0.8 2,311,485 - +0.5 2,311,485 - +1.7 2,311,485 - -1.8 2,311,485 - +1.4 2,311,485 - -2.0 2,311,485 - +1.3 2,311,485 - -2.6 2,311,485 - +1.4 2,311,488 + +1.2 2,311,494 + -0.6 2,311,497 - +0.0 2,311,502 - +4.1 2,311,528 + -0.0 2,311,582 + -0.1 2,311,590 - +0.4 2,311,590 - -1.3 2,311,590 - -0.3 2,311,657 - PGA1_c22240 0.14 -0.8 2,311,695 - PGA1_c22240 0.26 +1.2 2,311,749 + PGA1_c22240 0.44 +0.8 2,311,777 - PGA1_c22240 0.53 +1.0 2,311,836 - PGA1_c22240 0.73 -0.5 2,311,838 - PGA1_c22240 0.74 -1.4 2,312,082 + PGA1_c22250 0.25 -0.7 2,312,082 + PGA1_c22250 0.25 -3.2 2,312,118 + PGA1_c22250 0.31 -2.0 2,312,149 + PGA1_c22250 0.35 -3.8 2,312,153 + PGA1_c22250 0.36 +0.4 2,312,287 + PGA1_c22250 0.56 -1.0 2,312,311 + PGA1_c22250 0.60 -2.3 2,312,327 + PGA1_c22250 0.62 -2.8 2,312,400 - PGA1_c22250 0.73 -2.2 2,312,405 - PGA1_c22250 0.74 +0.4 2,312,438 - PGA1_c22250 0.79 +0.6 2,312,462 + PGA1_c22250 0.82 -1.2 2,312,540 - -2.8 2,312,540 - +0.6 2,312,540 - -0.7 2,312,566 - -0.2 2,312,629 + +1.2 2,312,632 - -0.2 2,312,637 - +1.2 2,312,773 - -2.2 2,312,778 - +1.3 2,312,839 - PGA1_c22260 0.19 +0.2 2,312,865 - PGA1_c22260 0.25 -0.8 2,312,886 + PGA1_c22260 0.31 -0.4 2,312,930 - PGA1_c22260 0.43 +0.7 2,312,949 - PGA1_c22260 0.48 +2.3 2,312,956 + PGA1_c22260 0.49 +1.2 2,312,974 - PGA1_c22260 0.54 -0.1 2,312,974 - PGA1_c22260 0.54 +0.5 2,312,984 + PGA1_c22260 0.57 +0.7 2,313,094 - PGA1_c22260 0.86 +0.7 2,313,154 + +0.3 2,313,223 + PGA1_c22270 0.19 -0.6 2,313,258 + PGA1_c22270 0.30 -2.5 2,313,258 + PGA1_c22270 0.30 -0.2 2,313,261 - PGA1_c22270 0.30 +0.5 2,313,263 + PGA1_c22270 0.31 -1.6 2,313,291 + PGA1_c22270 0.39 -2.2 2,313,291 + PGA1_c22270 0.39 +0.7 2,313,418 + PGA1_c22270 0.76 +1.3 2,313,418 + PGA1_c22270 0.76 +0.1 2,313,421 - PGA1_c22270 0.77 +0.6 2,313,450 + PGA1_c22270 0.85 +1.9 2,313,464 - PGA1_c22270 0.89 -0.3 2,313,486 + -0.0 2,313,518 + +0.2
Or see this region's nucleotide sequence