Experiment: m.b. nitrite 8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14610 and PGA1_c14630 are separated by 239 nucleotides PGA1_c14630 and PGA1_c14640 are separated by 100 nucleotides PGA1_c14640 and PGA1_c14650 are separated by 239 nucleotides
PGA1_c14610: PGA1_c14610 - GMP synthase, at 1,516,128 to 1,517,696
_c14610
PGA1_c14630: PGA1_c14630 - trimethylamine methyltransferase MttB, at 1,517,936 to 1,519,489
_c14630
PGA1_c14640: PGA1_c14640 - hypothetical protein, at 1,519,590 to 1,519,886
_c14640
PGA1_c14650: PGA1_c14650 - hypothetical protein, at 1,520,126 to 1,521,199
_c14650
Position (kb)
1517
1518
1519
1520 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1517.794 kb on - strand at 1517.812 kb on - strand at 1517.868 kb on - strand at 1517.885 kb on - strand at 1517.975 kb on + strand at 1517.985 kb on - strand at 1518.005 kb on + strand at 1518.005 kb on + strand at 1518.005 kb on + strand at 1518.005 kb on + strand at 1518.005 kb on + strand at 1518.005 kb on + strand at 1518.005 kb on + strand at 1518.319 kb on + strand, within PGA1_c14630 at 1518.328 kb on + strand, within PGA1_c14630 at 1518.356 kb on + strand, within PGA1_c14630 at 1518.383 kb on - strand, within PGA1_c14630 at 1518.420 kb on - strand, within PGA1_c14630 at 1518.593 kb on - strand, within PGA1_c14630 at 1518.616 kb on + strand, within PGA1_c14630 at 1518.624 kb on - strand, within PGA1_c14630 at 1518.699 kb on + strand, within PGA1_c14630 at 1518.705 kb on + strand, within PGA1_c14630 at 1518.710 kb on + strand, within PGA1_c14630 at 1518.832 kb on - strand, within PGA1_c14630 at 1518.909 kb on + strand, within PGA1_c14630 at 1518.909 kb on + strand, within PGA1_c14630 at 1518.944 kb on - strand, within PGA1_c14630 at 1518.988 kb on + strand, within PGA1_c14630 at 1519.004 kb on - strand, within PGA1_c14630 at 1519.053 kb on - strand, within PGA1_c14630 at 1519.171 kb on + strand, within PGA1_c14630 at 1519.237 kb on - strand, within PGA1_c14630 at 1519.366 kb on - strand at 1519.403 kb on - strand at 1519.550 kb on + strand at 1519.622 kb on + strand, within PGA1_c14640 at 1519.764 kb on + strand, within PGA1_c14640 at 1519.849 kb on - strand, within PGA1_c14640 at 1519.849 kb on - strand, within PGA1_c14640 at 1519.873 kb on + strand at 1519.873 kb on + strand at 1519.888 kb on + strand at 1519.920 kb on + strand at 1519.920 kb on + strand at 1519.920 kb on + strand at 1519.928 kb on - strand at 1520.014 kb on - strand at 1520.014 kb on - strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.110 kb on + strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.118 kb on - strand at 1520.141 kb on + strand at 1520.149 kb on - strand at 1520.192 kb on - strand at 1520.245 kb on + strand, within PGA1_c14650 at 1520.441 kb on + strand, within PGA1_c14650 at 1520.449 kb on - strand, within PGA1_c14650 at 1520.451 kb on + strand, within PGA1_c14650 at 1520.459 kb on - strand, within PGA1_c14650 at 1520.459 kb on - strand, within PGA1_c14650 at 1520.459 kb on - strand, within PGA1_c14650 at 1520.459 kb on - strand, within PGA1_c14650 at 1520.479 kb on - strand, within PGA1_c14650
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 8 mM remove 1,517,794 - -2.1 1,517,812 - +2.7 1,517,868 - -2.0 1,517,885 - -1.0 1,517,975 + -1.5 1,517,985 - -0.8 1,518,005 + -1.7 1,518,005 + -2.5 1,518,005 + -3.4 1,518,005 + -2.5 1,518,005 + -1.1 1,518,005 + -2.6 1,518,005 + -2.2 1,518,319 + PGA1_c14630 0.25 -3.5 1,518,328 + PGA1_c14630 0.25 -3.1 1,518,356 + PGA1_c14630 0.27 -2.5 1,518,383 - PGA1_c14630 0.29 -2.1 1,518,420 - PGA1_c14630 0.31 -2.2 1,518,593 - PGA1_c14630 0.42 -3.5 1,518,616 + PGA1_c14630 0.44 +1.5 1,518,624 - PGA1_c14630 0.44 -0.1 1,518,699 + PGA1_c14630 0.49 -0.7 1,518,705 + PGA1_c14630 0.49 -1.3 1,518,710 + PGA1_c14630 0.50 -3.2 1,518,832 - PGA1_c14630 0.58 -2.6 1,518,909 + PGA1_c14630 0.63 -3.8 1,518,909 + PGA1_c14630 0.63 -1.9 1,518,944 - PGA1_c14630 0.65 -2.1 1,518,988 + PGA1_c14630 0.68 -2.3 1,519,004 - PGA1_c14630 0.69 -3.7 1,519,053 - PGA1_c14630 0.72 -3.0 1,519,171 + PGA1_c14630 0.79 -2.4 1,519,237 - PGA1_c14630 0.84 -1.5 1,519,366 - -2.1 1,519,403 - -2.0 1,519,550 + +0.2 1,519,622 + PGA1_c14640 0.11 -0.7 1,519,764 + PGA1_c14640 0.59 +1.2 1,519,849 - PGA1_c14640 0.87 -1.2 1,519,849 - PGA1_c14640 0.87 -0.1 1,519,873 + -0.3 1,519,873 + +2.5 1,519,888 + -2.5 1,519,920 + +0.7 1,519,920 + -0.1 1,519,920 + -1.5 1,519,928 - -0.7 1,520,014 - -1.4 1,520,014 - -0.8 1,520,110 + +0.6 1,520,110 + -1.7 1,520,110 + -1.2 1,520,110 + -0.8 1,520,110 + +1.1 1,520,110 + +2.1 1,520,110 + -0.4 1,520,110 + -1.2 1,520,110 + +0.6 1,520,110 + -0.5 1,520,110 + -0.4 1,520,110 + -1.9 1,520,110 + +0.1 1,520,110 + -2.9 1,520,110 + -0.8 1,520,110 + +0.2 1,520,110 + -0.0 1,520,110 + +0.0 1,520,110 + +0.3 1,520,110 + +1.4 1,520,110 + +0.6 1,520,110 + -3.1 1,520,110 + +2.0 1,520,110 + +0.8 1,520,110 + +1.3 1,520,118 - +1.3 1,520,118 - -1.4 1,520,118 - +0.8 1,520,118 - -0.0 1,520,118 - +0.7 1,520,118 - -2.6 1,520,118 - +0.8 1,520,118 - -2.8 1,520,118 - +1.0 1,520,118 - -0.8 1,520,118 - -1.1 1,520,118 - +0.4 1,520,118 - -2.1 1,520,118 - +0.7 1,520,118 - -0.2 1,520,118 - -0.1 1,520,118 - +1.7 1,520,118 - +2.0 1,520,118 - -1.4 1,520,118 - -0.4 1,520,118 - +0.5 1,520,118 - +0.8 1,520,141 + -0.8 1,520,149 - -3.6 1,520,192 - +0.8 1,520,245 + PGA1_c14650 0.11 -2.0 1,520,441 + PGA1_c14650 0.29 +0.2 1,520,449 - PGA1_c14650 0.30 -1.4 1,520,451 + PGA1_c14650 0.30 +1.4 1,520,459 - PGA1_c14650 0.31 -0.9 1,520,459 - PGA1_c14650 0.31 +2.1 1,520,459 - PGA1_c14650 0.31 +1.2 1,520,459 - PGA1_c14650 0.31 +0.4 1,520,479 - PGA1_c14650 0.33 +0.5
Or see this region's nucleotide sequence