Experiment: m.b. nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09550 and PGA1_c09560 are separated by 124 nucleotides PGA1_c09560 and PGA1_c09570 are separated by 3 nucleotides PGA1_c09570 and PGA1_c09580 are separated by 4 nucleotides PGA1_c09580 and PGA1_c09590 are separated by 79 nucleotides
PGA1_c09550: PGA1_c09550 - Glutaminase (EC 3.5.1.2) <small>(from data)</small>, at 979,991 to 980,905
_c09550
PGA1_c09560: PGA1_c09560 - UDP-N-acetylmuramoylalanine--D-glutamate ligase MurD, at 981,030 to 982,427
_c09560
PGA1_c09570: PGA1_c09570 - putative HTH-type transcriptional regulator, MarR family, at 982,431 to 982,874
_c09570
PGA1_c09580: PGA1_c09580 - antibiotic biosynthesis monooxygenase-like protein, at 982,879 to 983,172
_c09580
PGA1_c09590: PGA1_c09590 - uncharacterized protein YhiN, at 983,252 to 984,433
_c09590
Position (kb) 981
982
983 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 980.066 kb on + strand at 980.074 kb on - strand at 980.113 kb on + strand, within PGA1_c09550 at 980.206 kb on - strand, within PGA1_c09550 at 980.238 kb on - strand, within PGA1_c09550 at 980.291 kb on - strand, within PGA1_c09550 at 980.358 kb on + strand, within PGA1_c09550 at 980.358 kb on + strand, within PGA1_c09550 at 980.400 kb on + strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.408 kb on - strand, within PGA1_c09550 at 980.487 kb on - strand, within PGA1_c09550 at 980.515 kb on + strand, within PGA1_c09550 at 980.515 kb on + strand, within PGA1_c09550 at 980.520 kb on + strand, within PGA1_c09550 at 980.536 kb on + strand, within PGA1_c09550 at 980.544 kb on + strand, within PGA1_c09550 at 980.556 kb on - strand, within PGA1_c09550 at 980.623 kb on - strand, within PGA1_c09550 at 980.635 kb on - strand, within PGA1_c09550 at 980.675 kb on + strand, within PGA1_c09550 at 980.675 kb on + strand, within PGA1_c09550 at 980.675 kb on + strand, within PGA1_c09550 at 980.683 kb on - strand, within PGA1_c09550 at 980.683 kb on - strand, within PGA1_c09550 at 980.683 kb on - strand, within PGA1_c09550 at 980.683 kb on - strand, within PGA1_c09550 at 980.701 kb on - strand, within PGA1_c09550 at 980.701 kb on - strand, within PGA1_c09550 at 980.719 kb on - strand, within PGA1_c09550 at 980.745 kb on + strand, within PGA1_c09550 at 980.745 kb on + strand, within PGA1_c09550 at 980.745 kb on + strand, within PGA1_c09550 at 980.753 kb on - strand, within PGA1_c09550 at 980.796 kb on + strand, within PGA1_c09550 at 980.802 kb on + strand, within PGA1_c09550 at 980.810 kb on - strand, within PGA1_c09550 at 980.847 kb on + strand at 980.850 kb on - strand at 980.855 kb on - strand at 980.938 kb on - strand at 981.020 kb on + strand at 982.421 kb on + strand at 982.423 kb on + strand at 982.423 kb on + strand at 982.531 kb on - strand, within PGA1_c09570 at 982.564 kb on + strand, within PGA1_c09570 at 982.590 kb on + strand, within PGA1_c09570 at 982.590 kb on + strand, within PGA1_c09570 at 982.594 kb on - strand, within PGA1_c09570 at 982.598 kb on - strand, within PGA1_c09570 at 982.608 kb on - strand, within PGA1_c09570 at 982.726 kb on - strand, within PGA1_c09570 at 982.727 kb on - strand, within PGA1_c09570 at 982.820 kb on + strand, within PGA1_c09570 at 982.828 kb on - strand, within PGA1_c09570 at 982.830 kb on + strand at 982.830 kb on + strand at 982.849 kb on - strand at 982.882 kb on + strand at 982.885 kb on - strand at 982.885 kb on - strand at 982.890 kb on - strand at 982.913 kb on + strand, within PGA1_c09580 at 982.913 kb on + strand, within PGA1_c09580 at 982.921 kb on - strand, within PGA1_c09580 at 983.013 kb on + strand, within PGA1_c09580 at 983.085 kb on - strand, within PGA1_c09580 at 983.132 kb on - strand, within PGA1_c09580 at 983.138 kb on - strand, within PGA1_c09580 at 983.250 kb on + strand at 983.250 kb on + strand at 983.258 kb on - strand at 983.284 kb on - strand at 983.297 kb on - strand at 983.297 kb on - strand at 983.330 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 6 mM remove 980,066 + -2.5 980,074 - +1.4 980,113 + PGA1_c09550 0.13 +1.4 980,206 - PGA1_c09550 0.23 -1.5 980,238 - PGA1_c09550 0.27 -0.3 980,291 - PGA1_c09550 0.33 -0.4 980,358 + PGA1_c09550 0.40 -0.5 980,358 + PGA1_c09550 0.40 +0.1 980,400 + PGA1_c09550 0.45 +1.1 980,408 - PGA1_c09550 0.46 -1.6 980,408 - PGA1_c09550 0.46 -0.2 980,408 - PGA1_c09550 0.46 -0.1 980,408 - PGA1_c09550 0.46 -1.0 980,487 - PGA1_c09550 0.54 -1.0 980,515 + PGA1_c09550 0.57 -1.2 980,515 + PGA1_c09550 0.57 +0.2 980,520 + PGA1_c09550 0.58 +0.9 980,536 + PGA1_c09550 0.60 -0.5 980,544 + PGA1_c09550 0.60 +0.7 980,556 - PGA1_c09550 0.62 -1.5 980,623 - PGA1_c09550 0.69 -0.5 980,635 - PGA1_c09550 0.70 -1.3 980,675 + PGA1_c09550 0.75 -1.2 980,675 + PGA1_c09550 0.75 -0.3 980,675 + PGA1_c09550 0.75 -1.2 980,683 - PGA1_c09550 0.76 -0.3 980,683 - PGA1_c09550 0.76 -0.1 980,683 - PGA1_c09550 0.76 -1.3 980,683 - PGA1_c09550 0.76 -1.2 980,701 - PGA1_c09550 0.78 +0.1 980,701 - PGA1_c09550 0.78 +2.0 980,719 - PGA1_c09550 0.80 +0.5 980,745 + PGA1_c09550 0.82 +0.5 980,745 + PGA1_c09550 0.82 -2.8 980,745 + PGA1_c09550 0.82 +0.7 980,753 - PGA1_c09550 0.83 -1.7 980,796 + PGA1_c09550 0.88 +0.1 980,802 + PGA1_c09550 0.89 +0.4 980,810 - PGA1_c09550 0.90 +0.3 980,847 + -1.7 980,850 - +1.8 980,855 - +1.4 980,938 - +0.7 981,020 + -0.1 982,421 + -0.1 982,423 + -0.1 982,423 + +0.1 982,531 - PGA1_c09570 0.23 -2.5 982,564 + PGA1_c09570 0.30 +0.3 982,590 + PGA1_c09570 0.36 -1.5 982,590 + PGA1_c09570 0.36 -0.3 982,594 - PGA1_c09570 0.37 -0.5 982,598 - PGA1_c09570 0.38 +0.4 982,608 - PGA1_c09570 0.40 +1.6 982,726 - PGA1_c09570 0.66 -0.2 982,727 - PGA1_c09570 0.67 +1.6 982,820 + PGA1_c09570 0.88 +1.0 982,828 - PGA1_c09570 0.89 -0.3 982,830 + -3.0 982,830 + +2.5 982,849 - +0.5 982,882 + +1.7 982,885 - +1.2 982,885 - -0.5 982,890 - +0.2 982,913 + PGA1_c09580 0.12 -0.6 982,913 + PGA1_c09580 0.12 -1.3 982,921 - PGA1_c09580 0.14 +1.4 983,013 + PGA1_c09580 0.46 -3.4 983,085 - PGA1_c09580 0.70 -1.0 983,132 - PGA1_c09580 0.86 +0.5 983,138 - PGA1_c09580 0.88 +1.2 983,250 + -3.6 983,250 + -0.5 983,258 - -0.9 983,284 - -1.0 983,297 - +0.9 983,297 - -2.0 983,330 + -0.4
Or see this region's nucleotide sequence