Experiment: m.b. nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00900 and PGA1_c00910 are separated by 12 nucleotides PGA1_c00910 and PGA1_c00920 are separated by 17 nucleotides PGA1_c00920 and PGA1_c00930 overlap by 4 nucleotides PGA1_c00930 and PGA1_c00940 overlap by 8 nucleotides
PGA1_c00900: PGA1_c00900 - methionyl-tRNA formyltransferase Fmt, at 87,820 to 88,725
_c00900
PGA1_c00910: PGA1_c00910 - hypothetical protein, at 88,738 to 88,974
_c00910
PGA1_c00920: PGA1_c00920 - putative transporter, required for glycine utilization (from data) , at 88,992 to 89,615
_c00920
PGA1_c00930: PGA1_c00930 - ribonuclease H, at 89,612 to 90,079
_c00930
PGA1_c00940: PGA1_c00940 - putative methyltransferase, at 90,072 to 90,671
_c00940
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 88.719 kb on + strand at 88.719 kb on - strand at 88.727 kb on + strand at 88.727 kb on - strand at 88.758 kb on - strand at 88.760 kb on + strand at 88.876 kb on + strand, within PGA1_c00910 at 88.884 kb on - strand, within PGA1_c00910 at 89.068 kb on - strand, within PGA1_c00920 at 89.072 kb on + strand, within PGA1_c00920 at 89.078 kb on - strand, within PGA1_c00920 at 89.080 kb on - strand, within PGA1_c00920 at 89.082 kb on + strand, within PGA1_c00920 at 89.082 kb on + strand, within PGA1_c00920 at 89.082 kb on + strand, within PGA1_c00920 at 89.088 kb on - strand, within PGA1_c00920 at 89.116 kb on - strand, within PGA1_c00920 at 89.116 kb on - strand, within PGA1_c00920 at 89.139 kb on - strand, within PGA1_c00920 at 89.186 kb on - strand, within PGA1_c00920 at 89.188 kb on + strand, within PGA1_c00920 at 89.271 kb on + strand, within PGA1_c00920 at 89.271 kb on + strand, within PGA1_c00920 at 89.271 kb on + strand, within PGA1_c00920 at 89.279 kb on - strand, within PGA1_c00920 at 89.302 kb on - strand, within PGA1_c00920 at 89.306 kb on - strand, within PGA1_c00920 at 89.369 kb on - strand, within PGA1_c00920 at 89.445 kb on - strand, within PGA1_c00920 at 89.558 kb on + strand at 89.558 kb on + strand at 89.576 kb on + strand at 89.579 kb on + strand at 89.600 kb on + strand at 89.609 kb on - strand at 89.639 kb on - strand at 89.640 kb on - strand at 89.647 kb on + strand at 89.826 kb on - strand, within PGA1_c00930 at 90.097 kb on - strand at 90.112 kb on - strand at 90.136 kb on + strand, within PGA1_c00940 at 90.142 kb on + strand, within PGA1_c00940 at 90.144 kb on - strand, within PGA1_c00940 at 90.205 kb on + strand, within PGA1_c00940 at 90.205 kb on + strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.213 kb on - strand, within PGA1_c00940 at 90.215 kb on + strand, within PGA1_c00940 at 90.215 kb on + strand, within PGA1_c00940 at 90.215 kb on + strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.223 kb on - strand, within PGA1_c00940 at 90.240 kb on - strand, within PGA1_c00940 at 90.249 kb on + strand, within PGA1_c00940 at 90.259 kb on + strand, within PGA1_c00940 at 90.259 kb on + strand, within PGA1_c00940 at 90.287 kb on - strand, within PGA1_c00940 at 90.351 kb on + strand, within PGA1_c00940 at 90.367 kb on - strand, within PGA1_c00940 at 90.397 kb on - strand, within PGA1_c00940 at 90.408 kb on + strand, within PGA1_c00940 at 90.473 kb on + strand, within PGA1_c00940 at 90.497 kb on - strand, within PGA1_c00940 at 90.499 kb on + strand, within PGA1_c00940 at 90.507 kb on - strand, within PGA1_c00940 at 90.530 kb on - strand, within PGA1_c00940 at 90.559 kb on - strand, within PGA1_c00940 at 90.601 kb on + strand, within PGA1_c00940
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 6 mM remove 88,719 + +0.2 88,719 - -0.9 88,727 + +0.5 88,727 - -0.0 88,758 - -0.0 88,760 + -3.1 88,876 + PGA1_c00910 0.58 +0.5 88,884 - PGA1_c00910 0.62 +0.1 89,068 - PGA1_c00920 0.12 +0.6 89,072 + PGA1_c00920 0.13 +0.3 89,078 - PGA1_c00920 0.14 -0.3 89,080 - PGA1_c00920 0.14 -0.3 89,082 + PGA1_c00920 0.14 +0.3 89,082 + PGA1_c00920 0.14 -2.1 89,082 + PGA1_c00920 0.14 -1.2 89,088 - PGA1_c00920 0.15 -1.7 89,116 - PGA1_c00920 0.20 -1.5 89,116 - PGA1_c00920 0.20 +0.8 89,139 - PGA1_c00920 0.24 -2.1 89,186 - PGA1_c00920 0.31 -1.1 89,188 + PGA1_c00920 0.31 +0.8 89,271 + PGA1_c00920 0.45 +1.0 89,271 + PGA1_c00920 0.45 +0.1 89,271 + PGA1_c00920 0.45 +0.8 89,279 - PGA1_c00920 0.46 -1.1 89,302 - PGA1_c00920 0.50 -1.3 89,306 - PGA1_c00920 0.50 -0.9 89,369 - PGA1_c00920 0.60 +1.3 89,445 - PGA1_c00920 0.73 +1.3 89,558 + +2.1 89,558 + +0.1 89,576 + -3.9 89,579 + -1.3 89,600 + -1.1 89,609 - +1.0 89,639 - -1.8 89,640 - +0.3 89,647 + -1.2 89,826 - PGA1_c00930 0.46 -1.5 90,097 - -0.5 90,112 - +3.8 90,136 + PGA1_c00940 0.11 -0.6 90,142 + PGA1_c00940 0.12 -0.3 90,144 - PGA1_c00940 0.12 -1.0 90,205 + PGA1_c00940 0.22 -1.5 90,205 + PGA1_c00940 0.22 +0.6 90,213 - PGA1_c00940 0.23 +0.1 90,213 - PGA1_c00940 0.23 -0.7 90,213 - PGA1_c00940 0.23 +0.5 90,213 - PGA1_c00940 0.23 -2.0 90,213 - PGA1_c00940 0.23 -0.7 90,213 - PGA1_c00940 0.23 -0.7 90,213 - PGA1_c00940 0.23 +0.5 90,213 - PGA1_c00940 0.23 -0.2 90,215 + PGA1_c00940 0.24 +0.7 90,215 + PGA1_c00940 0.24 +0.2 90,215 + PGA1_c00940 0.24 +0.2 90,223 - PGA1_c00940 0.25 -0.6 90,223 - PGA1_c00940 0.25 -1.3 90,223 - PGA1_c00940 0.25 +1.0 90,223 - PGA1_c00940 0.25 -0.4 90,223 - PGA1_c00940 0.25 +0.3 90,240 - PGA1_c00940 0.28 -1.0 90,249 + PGA1_c00940 0.29 +1.3 90,259 + PGA1_c00940 0.31 +0.8 90,259 + PGA1_c00940 0.31 -0.9 90,287 - PGA1_c00940 0.36 -0.8 90,351 + PGA1_c00940 0.47 -1.2 90,367 - PGA1_c00940 0.49 +1.0 90,397 - PGA1_c00940 0.54 -0.1 90,408 + PGA1_c00940 0.56 -1.0 90,473 + PGA1_c00940 0.67 -3.7 90,497 - PGA1_c00940 0.71 -0.1 90,499 + PGA1_c00940 0.71 -0.2 90,507 - PGA1_c00940 0.72 +0.6 90,530 - PGA1_c00940 0.76 +1.3 90,559 - PGA1_c00940 0.81 -0.1 90,601 + PGA1_c00940 0.88 -2.6
Or see this region's nucleotide sequence