Experiment: m.b. nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00020 and PGA1_65p00030 overlap by 4 nucleotides PGA1_65p00030 and PGA1_65p00040 are separated by 162 nucleotides
PGA1_65p00020: PGA1_65p00020 - putative type I secretion membrane fusion protein, at 1,376 to 2,779
_65p00020
PGA1_65p00030: PGA1_65p00030 - putative type I secretion system ATPase, at 2,776 to 4,539
_65p00030
PGA1_65p00040: PGA1_65p00040 - RTX toxins and related Ca2+-binding proteins, at 4,702 to 7,215
_65p00040
Position (kb) 2
3
4
5 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1.785 kb on - strand, within PGA1_65p00020 at 1.987 kb on + strand, within PGA1_65p00020 at 1.989 kb on - strand, within PGA1_65p00020 at 2.669 kb on - strand at 2.683 kb on + strand at 2.683 kb on + strand at 2.774 kb on + strand at 2.856 kb on + strand at 2.875 kb on - strand at 3.023 kb on - strand, within PGA1_65p00030 at 3.153 kb on - strand, within PGA1_65p00030 at 3.255 kb on + strand, within PGA1_65p00030 at 3.307 kb on + strand, within PGA1_65p00030 at 3.433 kb on - strand, within PGA1_65p00030 at 3.461 kb on + strand, within PGA1_65p00030 at 3.512 kb on + strand, within PGA1_65p00030 at 3.545 kb on + strand, within PGA1_65p00030 at 3.928 kb on + strand, within PGA1_65p00030 at 3.937 kb on - strand, within PGA1_65p00030 at 4.047 kb on + strand, within PGA1_65p00030 at 4.129 kb on + strand, within PGA1_65p00030 at 4.318 kb on + strand, within PGA1_65p00030 at 4.402 kb on + strand at 4.542 kb on - strand at 4.556 kb on - strand at 4.607 kb on + strand at 4.657 kb on - strand at 4.936 kb on + strand at 4.969 kb on + strand, within PGA1_65p00040 at 5.028 kb on - strand, within PGA1_65p00040 at 5.028 kb on - strand at 5.134 kb on - strand, within PGA1_65p00040 at 5.289 kb on - strand at 5.317 kb on - strand, within PGA1_65p00040 at 5.428 kb on + strand, within PGA1_65p00040 at 5.497 kb on + strand, within PGA1_65p00040
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 6 mM remove 1,785 - PGA1_65p00020 0.29 +1.4 1,987 + PGA1_65p00020 0.44 +0.4 1,989 - PGA1_65p00020 0.44 +1.3 2,669 - -0.9 2,683 + +0.7 2,683 + -0.3 2,774 + +1.7 2,856 + -1.4 2,875 - -1.1 3,023 - PGA1_65p00030 0.14 +1.3 3,153 - PGA1_65p00030 0.21 +3.2 3,255 + PGA1_65p00030 0.27 -2.6 3,307 + PGA1_65p00030 0.30 +2.1 3,433 - PGA1_65p00030 0.37 -0.3 3,461 + PGA1_65p00030 0.39 +1.2 3,512 + PGA1_65p00030 0.42 +1.1 3,545 + PGA1_65p00030 0.44 +1.1 3,928 + PGA1_65p00030 0.65 +1.4 3,937 - PGA1_65p00030 0.66 +0.4 4,047 + PGA1_65p00030 0.72 -0.9 4,129 + PGA1_65p00030 0.77 +0.5 4,318 + PGA1_65p00030 0.87 +1.5 4,402 + +0.4 4,542 - +2.0 4,556 - -0.3 4,607 + -2.1 4,657 - -1.0 4,936 + -0.4 4,969 + PGA1_65p00040 0.11 +0.4 5,028 - PGA1_65p00040 0.13 -1.9 5,028 - -1.6 5,134 - PGA1_65p00040 0.17 -0.5 5,289 - +1.3 5,317 - PGA1_65p00040 0.24 -1.1 5,428 + PGA1_65p00040 0.29 -1.4 5,497 + PGA1_65p00040 0.32 +2.1
Or see this region's nucleotide sequence