Experiment: m.b. nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03210 and PGA1_c03220 overlap by 4 nucleotides PGA1_c03220 and PGA1_c03230 are separated by 59 nucleotides PGA1_c03230 and PGA1_c03240 are separated by 62 nucleotides PGA1_c03240 and PGA1_c03250 overlap by 4 nucleotides
PGA1_c03210: PGA1_c03210 - branched-chain amino acid transport protein azlD-like protein, at 308,374 to 308,709
_c03210
PGA1_c03220: PGA1_c03220 - branched-chain amino acid transport protein azlC-like protein, at 308,706 to 309,431
_c03220
PGA1_c03230: PGA1_c03230 - putative formate dehydrogenase, subunit FdhD, at 309,491 to 310,393
_c03230
PGA1_c03240: PGA1_c03240 - probable molybdopterin-guanine dinucleotide biosynthesis protein A, at 310,456 to 311,097
_c03240
PGA1_c03250: PGA1_c03250 - molybdopterin-guanine dinucleotide biosynthesis protein B, at 311,094 to 311,585
_c03250
Position (kb) 309
310
311 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 308.524 kb on + strand, within PGA1_c03210 at 308.527 kb on + strand, within PGA1_c03210 at 308.567 kb on + strand, within PGA1_c03210 at 308.567 kb on - strand, within PGA1_c03210 at 308.575 kb on - strand, within PGA1_c03210 at 308.595 kb on + strand, within PGA1_c03210 at 308.648 kb on - strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.675 kb on + strand, within PGA1_c03210 at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.683 kb on - strand at 308.686 kb on + strand at 308.693 kb on - strand at 308.693 kb on - strand at 308.718 kb on - strand at 308.718 kb on - strand at 308.754 kb on - strand at 308.756 kb on + strand at 308.761 kb on - strand at 308.789 kb on - strand, within PGA1_c03220 at 308.803 kb on - strand at 308.816 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.826 kb on - strand, within PGA1_c03220 at 308.868 kb on - strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.879 kb on + strand, within PGA1_c03220 at 308.887 kb on - strand, within PGA1_c03220 at 308.957 kb on + strand, within PGA1_c03220 at 308.975 kb on - strand, within PGA1_c03220 at 308.981 kb on - strand, within PGA1_c03220 at 309.005 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.035 kb on + strand, within PGA1_c03220 at 309.043 kb on - strand, within PGA1_c03220 at 309.068 kb on + strand, within PGA1_c03220 at 309.068 kb on + strand, within PGA1_c03220 at 309.082 kb on + strand, within PGA1_c03220 at 309.090 kb on - strand, within PGA1_c03220 at 309.154 kb on - strand, within PGA1_c03220 at 309.250 kb on + strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.258 kb on - strand, within PGA1_c03220 at 309.268 kb on - strand, within PGA1_c03220 at 309.280 kb on - strand, within PGA1_c03220 at 309.304 kb on + strand, within PGA1_c03220 at 309.306 kb on - strand, within PGA1_c03220 at 309.459 kb on - strand at 309.460 kb on + strand at 309.460 kb on + strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.468 kb on - strand at 309.515 kb on - strand at 309.520 kb on - strand at 309.739 kb on + strand, within PGA1_c03230 at 309.749 kb on + strand, within PGA1_c03230 at 309.782 kb on + strand, within PGA1_c03230 at 309.827 kb on - strand, within PGA1_c03230 at 309.874 kb on - strand, within PGA1_c03230 at 309.927 kb on + strand, within PGA1_c03230 at 309.927 kb on + strand, within PGA1_c03230 at 309.929 kb on + strand, within PGA1_c03230 at 309.929 kb on + strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.937 kb on - strand, within PGA1_c03230 at 309.986 kb on - strand, within PGA1_c03230 at 309.990 kb on + strand, within PGA1_c03230 at 309.990 kb on + strand, within PGA1_c03230 at 309.998 kb on - strand, within PGA1_c03230 at 309.998 kb on - strand, within PGA1_c03230 at 310.026 kb on + strand, within PGA1_c03230 at 310.045 kb on + strand, within PGA1_c03230 at 310.069 kb on + strand, within PGA1_c03230 at 310.153 kb on + strand, within PGA1_c03230 at 310.158 kb on + strand, within PGA1_c03230 at 310.166 kb on - strand, within PGA1_c03230 at 310.299 kb on - strand, within PGA1_c03230 at 310.348 kb on - strand at 310.440 kb on + strand at 310.639 kb on + strand, within PGA1_c03240 at 310.675 kb on - strand, within PGA1_c03240 at 310.707 kb on - strand, within PGA1_c03240 at 310.721 kb on - strand, within PGA1_c03240 at 310.811 kb on + strand, within PGA1_c03240 at 310.816 kb on + strand, within PGA1_c03240 at 310.922 kb on + strand, within PGA1_c03240 at 310.971 kb on - strand, within PGA1_c03240 at 310.981 kb on - strand, within PGA1_c03240 at 310.990 kb on + strand, within PGA1_c03240 at 310.998 kb on - strand, within PGA1_c03240 at 311.024 kb on - strand, within PGA1_c03240 at 311.024 kb on - strand, within PGA1_c03240 at 311.049 kb on + strand at 311.057 kb on - strand at 311.076 kb on + strand at 311.099 kb on + strand at 311.099 kb on + strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.107 kb on - strand at 311.109 kb on + strand at 311.124 kb on - strand at 311.179 kb on + strand, within PGA1_c03250 at 311.208 kb on + strand, within PGA1_c03250 at 311.221 kb on - strand, within PGA1_c03250 at 311.270 kb on + strand, within PGA1_c03250 at 311.331 kb on + strand, within PGA1_c03250 at 311.376 kb on + strand, within PGA1_c03250
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 6 mM remove 308,524 + PGA1_c03210 0.45 +0.5 308,527 + PGA1_c03210 0.46 +0.6 308,567 + PGA1_c03210 0.57 +0.2 308,567 - PGA1_c03210 0.57 +0.4 308,575 - PGA1_c03210 0.60 +2.3 308,595 + PGA1_c03210 0.66 -2.0 308,648 - PGA1_c03210 0.82 +0.2 308,675 + PGA1_c03210 0.90 +0.8 308,675 + PGA1_c03210 0.90 +0.2 308,675 + PGA1_c03210 0.90 +0.1 308,675 + PGA1_c03210 0.90 -0.3 308,675 + PGA1_c03210 0.90 -0.5 308,675 + PGA1_c03210 0.90 +0.1 308,683 - +0.5 308,683 - -2.0 308,683 - -0.5 308,683 - +0.6 308,683 - -0.6 308,686 + +0.5 308,693 - -2.4 308,693 - -2.2 308,718 - +1.1 308,718 - +0.5 308,754 - +0.9 308,756 + -1.6 308,761 - -2.0 308,789 - PGA1_c03220 0.11 +0.8 308,803 - +1.3 308,816 - PGA1_c03220 0.15 -2.7 308,826 - PGA1_c03220 0.17 +1.2 308,826 - PGA1_c03220 0.17 +0.8 308,826 - PGA1_c03220 0.17 -0.3 308,826 - PGA1_c03220 0.17 -0.3 308,826 - PGA1_c03220 0.17 +0.7 308,868 - PGA1_c03220 0.22 -0.4 308,879 + PGA1_c03220 0.24 -1.3 308,879 + PGA1_c03220 0.24 -1.1 308,879 + PGA1_c03220 0.24 -0.3 308,887 - PGA1_c03220 0.25 -0.8 308,957 + PGA1_c03220 0.35 +0.4 308,975 - PGA1_c03220 0.37 +0.5 308,981 - PGA1_c03220 0.38 +1.5 309,005 + PGA1_c03220 0.41 +0.4 309,035 + PGA1_c03220 0.45 -0.8 309,035 + PGA1_c03220 0.45 +0.9 309,035 + PGA1_c03220 0.45 +0.0 309,043 - PGA1_c03220 0.46 -1.3 309,068 + PGA1_c03220 0.50 +0.9 309,068 + PGA1_c03220 0.50 -2.9 309,082 + PGA1_c03220 0.52 -2.8 309,090 - PGA1_c03220 0.53 -0.4 309,154 - PGA1_c03220 0.62 +0.7 309,250 + PGA1_c03220 0.75 +0.5 309,258 - PGA1_c03220 0.76 -1.3 309,258 - PGA1_c03220 0.76 +0.1 309,258 - PGA1_c03220 0.76 +0.2 309,268 - PGA1_c03220 0.77 +0.4 309,280 - PGA1_c03220 0.79 -0.5 309,304 + PGA1_c03220 0.82 -0.7 309,306 - PGA1_c03220 0.83 +1.7 309,459 - -2.6 309,460 + +3.4 309,460 + +1.3 309,468 - -1.0 309,468 - +1.3 309,468 - -1.5 309,468 - +1.2 309,515 - +1.7 309,520 - +1.1 309,739 + PGA1_c03230 0.27 -1.5 309,749 + PGA1_c03230 0.29 +1.9 309,782 + PGA1_c03230 0.32 +1.5 309,827 - PGA1_c03230 0.37 -3.6 309,874 - PGA1_c03230 0.42 -1.1 309,927 + PGA1_c03230 0.48 +1.3 309,927 + PGA1_c03230 0.48 +0.4 309,929 + PGA1_c03230 0.49 -1.4 309,929 + PGA1_c03230 0.49 +1.3 309,937 - PGA1_c03230 0.49 +2.6 309,937 - PGA1_c03230 0.49 +0.4 309,937 - PGA1_c03230 0.49 +2.0 309,986 - PGA1_c03230 0.55 +1.3 309,990 + PGA1_c03230 0.55 +0.6 309,990 + PGA1_c03230 0.55 -1.7 309,998 - PGA1_c03230 0.56 +2.1 309,998 - PGA1_c03230 0.56 +0.6 310,026 + PGA1_c03230 0.59 -1.3 310,045 + PGA1_c03230 0.61 +0.5 310,069 + PGA1_c03230 0.64 +0.8 310,153 + PGA1_c03230 0.73 -2.2 310,158 + PGA1_c03230 0.74 +1.3 310,166 - PGA1_c03230 0.75 +0.9 310,299 - PGA1_c03230 0.89 +1.0 310,348 - +0.8 310,440 + -1.0 310,639 + PGA1_c03240 0.29 +2.3 310,675 - PGA1_c03240 0.34 +0.5 310,707 - PGA1_c03240 0.39 +2.1 310,721 - PGA1_c03240 0.41 -0.8 310,811 + PGA1_c03240 0.55 +2.0 310,816 + PGA1_c03240 0.56 -2.9 310,922 + PGA1_c03240 0.73 +1.7 310,971 - PGA1_c03240 0.80 -1.3 310,981 - PGA1_c03240 0.82 -1.0 310,990 + PGA1_c03240 0.83 +1.1 310,998 - PGA1_c03240 0.84 +0.5 311,024 - PGA1_c03240 0.88 +1.9 311,024 - PGA1_c03240 0.88 +0.8 311,049 + -0.7 311,057 - +0.2 311,076 + +0.9 311,099 + +1.2 311,099 + +1.0 311,107 - -0.2 311,107 - -0.4 311,107 - +2.0 311,107 - -2.9 311,107 - +1.4 311,109 + +1.1 311,124 - -0.3 311,179 + PGA1_c03250 0.17 -3.7 311,208 + PGA1_c03250 0.23 -2.3 311,221 - PGA1_c03250 0.26 -1.1 311,270 + PGA1_c03250 0.36 +1.3 311,331 + PGA1_c03250 0.48 -1.3 311,376 + PGA1_c03250 0.57 -0.1
Or see this region's nucleotide sequence