Experiment: m.b. nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c29920 and PGA1_c29930 are separated by 1 nucleotides PGA1_c29930 and PGA1_c29940 are separated by 99 nucleotides PGA1_c29940 and PGA1_c29950 are separated by 110 nucleotides
PGA1_c29920: PGA1_c29920 - alpha-methylacyl-CoA racemase, at 3,129,536 to 3,130,636
_c29920
PGA1_c29930: PGA1_c29930 - hypothetical protein, at 3,130,638 to 3,130,805
_c29930
PGA1_c29940: PGA1_c29940 - uroporphyrinogen decarboxylase HemE, at 3,130,905 to 3,131,939
_c29940
PGA1_c29950: PGA1_c29950 - porphobilinogen deaminase HemC, at 3,132,050 to 3,133,012
_c29950
Position (kb) 3130
3131
3132 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 3129.916 kb on + strand, within PGA1_c29920 at 3129.916 kb on + strand, within PGA1_c29920 at 3129.989 kb on + strand, within PGA1_c29920 at 3130.014 kb on + strand, within PGA1_c29920 at 3130.046 kb on + strand, within PGA1_c29920 at 3130.046 kb on + strand, within PGA1_c29920 at 3130.114 kb on - strand, within PGA1_c29920 at 3130.115 kb on + strand, within PGA1_c29920 at 3130.116 kb on + strand, within PGA1_c29920 at 3130.127 kb on + strand, within PGA1_c29920 at 3130.138 kb on + strand, within PGA1_c29920 at 3130.146 kb on - strand, within PGA1_c29920 at 3130.146 kb on - strand, within PGA1_c29920 at 3130.154 kb on - strand, within PGA1_c29920 at 3130.164 kb on - strand, within PGA1_c29920 at 3130.182 kb on + strand, within PGA1_c29920 at 3130.182 kb on + strand, within PGA1_c29920 at 3130.190 kb on - strand, within PGA1_c29920 at 3130.205 kb on + strand, within PGA1_c29920 at 3130.208 kb on - strand, within PGA1_c29920 at 3130.213 kb on - strand, within PGA1_c29920 at 3130.227 kb on + strand, within PGA1_c29920 at 3130.238 kb on + strand, within PGA1_c29920 at 3130.246 kb on - strand, within PGA1_c29920 at 3130.265 kb on - strand, within PGA1_c29920 at 3130.293 kb on + strand, within PGA1_c29920 at 3130.303 kb on + strand, within PGA1_c29920 at 3130.414 kb on + strand, within PGA1_c29920 at 3130.448 kb on - strand, within PGA1_c29920 at 3130.499 kb on - strand, within PGA1_c29920 at 3130.516 kb on + strand, within PGA1_c29920 at 3130.547 kb on + strand at 3130.555 kb on - strand at 3130.555 kb on - strand at 3130.603 kb on + strand at 3130.614 kb on + strand at 3130.618 kb on + strand at 3130.633 kb on - strand at 3130.669 kb on + strand, within PGA1_c29930 at 3130.669 kb on + strand, within PGA1_c29930 at 3130.677 kb on - strand, within PGA1_c29930 at 3130.677 kb on - strand, within PGA1_c29930 at 3130.677 kb on - strand, within PGA1_c29930 at 3130.682 kb on - strand, within PGA1_c29930 at 3130.745 kb on + strand, within PGA1_c29930 at 3130.745 kb on + strand, within PGA1_c29930 at 3130.745 kb on + strand, within PGA1_c29930 at 3130.753 kb on - strand, within PGA1_c29930 at 3130.767 kb on - strand, within PGA1_c29930 at 3130.807 kb on + strand at 3130.856 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 6 mM remove 3,129,916 + PGA1_c29920 0.35 +0.5 3,129,916 + PGA1_c29920 0.35 -0.0 3,129,989 + PGA1_c29920 0.41 -1.9 3,130,014 + PGA1_c29920 0.43 -1.7 3,130,046 + PGA1_c29920 0.46 -1.6 3,130,046 + PGA1_c29920 0.46 +0.0 3,130,114 - PGA1_c29920 0.52 +1.0 3,130,115 + PGA1_c29920 0.53 -1.2 3,130,116 + PGA1_c29920 0.53 +0.4 3,130,127 + PGA1_c29920 0.54 -0.2 3,130,138 + PGA1_c29920 0.55 +1.8 3,130,146 - PGA1_c29920 0.55 +0.0 3,130,146 - PGA1_c29920 0.55 +0.5 3,130,154 - PGA1_c29920 0.56 +0.1 3,130,164 - PGA1_c29920 0.57 +0.8 3,130,182 + PGA1_c29920 0.59 +0.2 3,130,182 + PGA1_c29920 0.59 -0.9 3,130,190 - PGA1_c29920 0.59 +0.7 3,130,205 + PGA1_c29920 0.61 -0.3 3,130,208 - PGA1_c29920 0.61 +0.5 3,130,213 - PGA1_c29920 0.61 -1.2 3,130,227 + PGA1_c29920 0.63 -0.5 3,130,238 + PGA1_c29920 0.64 +1.3 3,130,246 - PGA1_c29920 0.64 +2.2 3,130,265 - PGA1_c29920 0.66 -0.8 3,130,293 + PGA1_c29920 0.69 +1.9 3,130,303 + PGA1_c29920 0.70 +1.3 3,130,414 + PGA1_c29920 0.80 +0.1 3,130,448 - PGA1_c29920 0.83 +1.2 3,130,499 - PGA1_c29920 0.87 -0.8 3,130,516 + PGA1_c29920 0.89 +1.4 3,130,547 + +0.7 3,130,555 - +1.2 3,130,555 - +1.7 3,130,603 + +0.3 3,130,614 + +0.2 3,130,618 + +0.6 3,130,633 - +0.9 3,130,669 + PGA1_c29930 0.18 +1.1 3,130,669 + PGA1_c29930 0.18 +0.2 3,130,677 - PGA1_c29930 0.23 -2.2 3,130,677 - PGA1_c29930 0.23 -0.5 3,130,677 - PGA1_c29930 0.23 -2.2 3,130,682 - PGA1_c29930 0.26 -1.1 3,130,745 + PGA1_c29930 0.64 -2.0 3,130,745 + PGA1_c29930 0.64 -3.9 3,130,745 + PGA1_c29930 0.64 -1.4 3,130,753 - PGA1_c29930 0.68 -0.7 3,130,767 - PGA1_c29930 0.77 +0.2 3,130,807 + +0.9 3,130,856 - -1.8
Or see this region's nucleotide sequence