Experiment: m.b. nitrite 6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c01050 and PGA1_c01060 overlap by 4 nucleotides PGA1_c01060 and PGA1_c01070 overlap by 4 nucleotides PGA1_c01070 and PGA1_c01080 are separated by 6 nucleotides PGA1_c01080 and PGA1_c01090 are separated by 2 nucleotides
PGA1_c01050: PGA1_c01050 - putative signal peptidase I, at 98,997 to 99,833
_c01050
PGA1_c01060: PGA1_c01060 - ribonuclease 3, at 99,830 to 100,516
_c01060
PGA1_c01070: PGA1_c01070 - GTP-binding protein Era, at 100,513 to 101,418
_c01070
PGA1_c01080: PGA1_c01080 - Uncharacterized conserved protein, at 101,425 to 101,754
_c01080
PGA1_c01090: PGA1_c01090 - DNA repair protein RecO, at 101,757 to 102,482
_c01090
Position (kb) 100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 99.838 kb on + strand at 99.846 kb on - strand at 99.846 kb on - strand at 99.999 kb on - strand, within PGA1_c01060 at 100.182 kb on - strand, within PGA1_c01060 at 100.198 kb on + strand, within PGA1_c01060 at 100.255 kb on + strand, within PGA1_c01060 at 100.263 kb on - strand, within PGA1_c01060 at 100.300 kb on + strand, within PGA1_c01060 at 100.345 kb on + strand, within PGA1_c01060 at 100.425 kb on + strand, within PGA1_c01060 at 100.425 kb on + strand, within PGA1_c01060 at 101.399 kb on + strand at 101.444 kb on + strand at 101.465 kb on - strand, within PGA1_c01080 at 101.465 kb on - strand, within PGA1_c01080 at 101.569 kb on - strand, within PGA1_c01080 at 101.593 kb on + strand, within PGA1_c01080 at 101.595 kb on + strand, within PGA1_c01080 at 101.630 kb on + strand, within PGA1_c01080 at 101.738 kb on + strand at 101.743 kb on + strand at 101.743 kb on + strand at 101.748 kb on + strand at 101.860 kb on - strand, within PGA1_c01090 at 101.860 kb on - strand, within PGA1_c01090 at 101.879 kb on + strand, within PGA1_c01090 at 101.903 kb on - strand, within PGA1_c01090 at 102.086 kb on + strand, within PGA1_c01090 at 102.117 kb on + strand, within PGA1_c01090 at 102.163 kb on + strand, within PGA1_c01090 at 102.224 kb on - strand, within PGA1_c01090 at 102.224 kb on - strand, within PGA1_c01090 at 102.236 kb on - strand, within PGA1_c01090 at 102.270 kb on + strand, within PGA1_c01090 at 102.278 kb on - strand, within PGA1_c01090 at 102.278 kb on - strand, within PGA1_c01090 at 102.300 kb on + strand, within PGA1_c01090 at 102.313 kb on - strand, within PGA1_c01090 at 102.332 kb on - strand, within PGA1_c01090 at 102.372 kb on + strand, within PGA1_c01090 at 102.379 kb on + strand, within PGA1_c01090 at 102.392 kb on - strand, within PGA1_c01090
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrite 6 mM remove 99,838 + -1.4 99,846 - +0.4 99,846 - +1.9 99,999 - PGA1_c01060 0.25 -3.6 100,182 - PGA1_c01060 0.51 -1.1 100,198 + PGA1_c01060 0.54 +1.0 100,255 + PGA1_c01060 0.62 -0.3 100,263 - PGA1_c01060 0.63 +1.8 100,300 + PGA1_c01060 0.68 -2.7 100,345 + PGA1_c01060 0.75 +0.1 100,425 + PGA1_c01060 0.87 +1.0 100,425 + PGA1_c01060 0.87 -0.2 101,399 + -0.4 101,444 + +1.2 101,465 - PGA1_c01080 0.12 +2.0 101,465 - PGA1_c01080 0.12 -0.7 101,569 - PGA1_c01080 0.44 -1.8 101,593 + PGA1_c01080 0.51 +2.3 101,595 + PGA1_c01080 0.52 +2.3 101,630 + PGA1_c01080 0.62 +1.7 101,738 + -0.5 101,743 + +1.8 101,743 + +1.1 101,748 + +1.3 101,860 - PGA1_c01090 0.14 +1.3 101,860 - PGA1_c01090 0.14 -0.5 101,879 + PGA1_c01090 0.17 -0.5 101,903 - PGA1_c01090 0.20 -2.9 102,086 + PGA1_c01090 0.45 +0.1 102,117 + PGA1_c01090 0.50 -2.1 102,163 + PGA1_c01090 0.56 -1.8 102,224 - PGA1_c01090 0.64 -0.6 102,224 - PGA1_c01090 0.64 +2.4 102,236 - PGA1_c01090 0.66 +0.9 102,270 + PGA1_c01090 0.71 -0.4 102,278 - PGA1_c01090 0.72 -0.4 102,278 - PGA1_c01090 0.72 -2.6 102,300 + PGA1_c01090 0.75 -1.2 102,313 - PGA1_c01090 0.77 -2.2 102,332 - PGA1_c01090 0.79 +0.5 102,372 + PGA1_c01090 0.85 +2.0 102,379 + PGA1_c01090 0.86 +2.1 102,392 - PGA1_c01090 0.87 -3.0
Or see this region's nucleotide sequence