Experiment: Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxB and rnhB overlap by 1 nucleotides rnhB and dnaE are separated by 159 nucleotides dnaE and Sama_1155 are separated by 10 nucleotides
Sama_1152: lpxB - lipid-A-disaccharide synthase (RefSeq), at 1,402,156 to 1,403,337
lpxB
Sama_1153: rnhB - ribonuclease HII (RefSeq), at 1,403,337 to 1,403,957
rnhB
Sama_1154: dnaE - DNA polymerase III subunit alpha (RefSeq), at 1,404,117 to 1,407,590
dnaE
Sama_1155: Sama_1155 - cell cycle protein MesJ (RefSeq), at 1,407,601 to 1,409,094
_1155
Position (kb)
1404
1405
1406
1407
1408 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1403.348 kb on + strand at 1403.348 kb on + strand at 1403.456 kb on + strand, within rnhB at 1403.456 kb on + strand, within rnhB at 1403.532 kb on + strand, within rnhB at 1403.543 kb on + strand, within rnhB at 1403.543 kb on + strand, within rnhB at 1403.550 kb on - strand, within rnhB at 1403.603 kb on + strand, within rnhB at 1403.603 kb on + strand, within rnhB at 1403.603 kb on + strand, within rnhB at 1403.604 kb on - strand, within rnhB at 1403.604 kb on - strand, within rnhB at 1403.606 kb on + strand, within rnhB at 1403.710 kb on + strand, within rnhB at 1403.710 kb on + strand, within rnhB at 1403.725 kb on + strand, within rnhB at 1403.981 kb on + strand at 1403.981 kb on + strand at 1403.982 kb on - strand at 1403.982 kb on - strand at 1403.982 kb on - strand at 1404.002 kb on + strand at 1404.002 kb on + strand at 1404.003 kb on - strand at 1404.005 kb on + strand at 1404.005 kb on + strand at 1404.043 kb on - strand at 1404.060 kb on + strand at 1404.060 kb on + strand at 1404.072 kb on + strand at 1407.588 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml remove 1,403,348 + -2.0 1,403,348 + +0.2 1,403,456 + rnhB Sama_1153 0.19 -0.5 1,403,456 + rnhB Sama_1153 0.19 +0.0 1,403,532 + rnhB Sama_1153 0.31 +0.1 1,403,543 + rnhB Sama_1153 0.33 -0.1 1,403,543 + rnhB Sama_1153 0.33 -0.6 1,403,550 - rnhB Sama_1153 0.34 -0.1 1,403,603 + rnhB Sama_1153 0.43 +0.4 1,403,603 + rnhB Sama_1153 0.43 +0.6 1,403,603 + rnhB Sama_1153 0.43 -1.7 1,403,604 - rnhB Sama_1153 0.43 +1.3 1,403,604 - rnhB Sama_1153 0.43 +0.2 1,403,606 + rnhB Sama_1153 0.43 -0.7 1,403,710 + rnhB Sama_1153 0.60 -2.1 1,403,710 + rnhB Sama_1153 0.60 -0.1 1,403,725 + rnhB Sama_1153 0.62 +0.2 1,403,981 + +3.8 1,403,981 + +1.6 1,403,982 - -0.6 1,403,982 - -0.6 1,403,982 - +0.0 1,404,002 + -0.4 1,404,002 + -1.1 1,404,003 - -0.1 1,404,005 + -0.8 1,404,005 + -1.6 1,404,043 - -0.1 1,404,060 + -0.4 1,404,060 + -1.8 1,404,072 + +0.2 1,407,588 + -2.0
Or see this region's nucleotide sequence