Experiment: Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1084 and Sama_1085 are separated by 249 nucleotides Sama_1085 and Sama_1086 are separated by 204 nucleotides Sama_1086 and Sama_1087 are separated by 84 nucleotides Sama_1087 and Sama_1088 are separated by 11 nucleotides
Sama_1084: Sama_1084 - GGDEF domain-containing protein (RefSeq), at 1,311,233 to 1,312,921
_1084
Sama_1085: Sama_1085 - hypothetical protein (RefSeq), at 1,313,171 to 1,313,332
_1085
Sama_1086: Sama_1086 - Fmu (Sun) (RefSeq), at 1,313,537 to 1,314,901
_1086
Sama_1087: Sama_1087 - glutathione S-transferase family protein (RefSeq), at 1,314,986 to 1,315,654
_1087
Sama_1088: Sama_1088 - glutathione S-transferase family protein (RefSeq), at 1,315,666 to 1,316,295
_1088
Position (kb)
1313
1314
1315 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1312.570 kb on + strand, within Sama_1084 at 1312.570 kb on + strand, within Sama_1084 at 1312.639 kb on + strand, within Sama_1084 at 1312.639 kb on + strand, within Sama_1084 at 1312.639 kb on + strand, within Sama_1084 at 1312.639 kb on + strand, within Sama_1084 at 1312.639 kb on + strand, within Sama_1084 at 1312.639 kb on + strand, within Sama_1084 at 1312.719 kb on + strand, within Sama_1084 at 1312.719 kb on + strand, within Sama_1084 at 1312.720 kb on - strand, within Sama_1084 at 1312.749 kb on + strand, within Sama_1084 at 1312.861 kb on + strand at 1312.861 kb on + strand at 1312.861 kb on + strand at 1312.861 kb on + strand at 1312.861 kb on + strand at 1312.861 kb on + strand at 1312.861 kb on + strand at 1312.862 kb on - strand at 1312.862 kb on - strand at 1312.862 kb on - strand at 1312.862 kb on - strand at 1312.862 kb on - strand at 1312.862 kb on - strand at 1312.865 kb on + strand at 1312.865 kb on + strand at 1312.866 kb on - strand at 1312.968 kb on - strand at 1312.968 kb on - strand at 1312.997 kb on + strand at 1313.028 kb on - strand at 1313.038 kb on + strand at 1313.060 kb on + strand at 1313.060 kb on + strand at 1313.060 kb on + strand at 1313.061 kb on - strand at 1313.193 kb on + strand, within Sama_1085 at 1313.193 kb on + strand, within Sama_1085 at 1313.194 kb on - strand, within Sama_1085 at 1313.194 kb on - strand, within Sama_1085 at 1313.194 kb on - strand, within Sama_1085 at 1313.221 kb on - strand, within Sama_1085 at 1313.243 kb on - strand, within Sama_1085 at 1313.243 kb on - strand, within Sama_1085 at 1313.261 kb on - strand, within Sama_1085 at 1313.319 kb on + strand at 1313.319 kb on + strand at 1313.380 kb on + strand at 1313.402 kb on - strand at 1313.416 kb on - strand at 1313.460 kb on - strand at 1313.460 kb on - strand at 1313.460 kb on - strand at 1313.478 kb on + strand at 1313.479 kb on - strand at 1313.566 kb on + strand at 1313.566 kb on + strand at 1313.725 kb on - strand, within Sama_1086 at 1313.804 kb on - strand, within Sama_1086 at 1313.858 kb on - strand, within Sama_1086 at 1313.895 kb on + strand, within Sama_1086 at 1313.895 kb on + strand, within Sama_1086 at 1313.896 kb on - strand, within Sama_1086 at 1313.896 kb on - strand, within Sama_1086 at 1313.896 kb on - strand, within Sama_1086 at 1314.004 kb on + strand, within Sama_1086 at 1314.004 kb on + strand, within Sama_1086 at 1314.005 kb on - strand, within Sama_1086 at 1314.005 kb on - strand, within Sama_1086 at 1314.070 kb on + strand, within Sama_1086 at 1314.071 kb on - strand, within Sama_1086 at 1314.178 kb on + strand, within Sama_1086 at 1314.178 kb on + strand, within Sama_1086 at 1314.178 kb on + strand, within Sama_1086 at 1314.180 kb on + strand, within Sama_1086 at 1314.181 kb on - strand, within Sama_1086 at 1314.181 kb on - strand, within Sama_1086 at 1314.181 kb on - strand, within Sama_1086 at 1314.181 kb on - strand, within Sama_1086 at 1314.181 kb on - strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.244 kb on + strand, within Sama_1086 at 1314.245 kb on - strand, within Sama_1086 at 1314.245 kb on - strand, within Sama_1086 at 1314.245 kb on - strand, within Sama_1086 at 1314.445 kb on + strand, within Sama_1086 at 1314.446 kb on - strand, within Sama_1086 at 1314.446 kb on - strand, within Sama_1086 at 1314.446 kb on - strand, within Sama_1086 at 1314.450 kb on - strand, within Sama_1086 at 1314.450 kb on - strand, within Sama_1086 at 1314.450 kb on - strand, within Sama_1086 at 1314.450 kb on - strand, within Sama_1086 at 1314.485 kb on - strand, within Sama_1086 at 1314.587 kb on + strand, within Sama_1086 at 1314.588 kb on - strand, within Sama_1086 at 1314.588 kb on - strand, within Sama_1086 at 1314.588 kb on - strand, within Sama_1086 at 1314.588 kb on - strand, within Sama_1086 at 1314.596 kb on - strand, within Sama_1086 at 1314.652 kb on + strand, within Sama_1086 at 1314.652 kb on + strand, within Sama_1086 at 1314.652 kb on + strand, within Sama_1086 at 1314.653 kb on - strand, within Sama_1086 at 1314.739 kb on + strand, within Sama_1086 at 1314.739 kb on + strand, within Sama_1086 at 1314.739 kb on + strand, within Sama_1086 at 1314.739 kb on + strand, within Sama_1086 at 1314.739 kb on + strand, within Sama_1086 at 1314.740 kb on - strand, within Sama_1086 at 1314.740 kb on - strand, within Sama_1086 at 1314.740 kb on - strand, within Sama_1086 at 1314.740 kb on - strand, within Sama_1086 at 1314.777 kb on + strand at 1314.777 kb on + strand at 1314.777 kb on + strand at 1314.815 kb on - strand at 1314.815 kb on - strand at 1314.852 kb on + strand at 1314.852 kb on + strand at 1314.852 kb on + strand at 1314.853 kb on - strand at 1315.129 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.149 kb on + strand, within Sama_1087 at 1315.150 kb on - strand, within Sama_1087 at 1315.150 kb on - strand, within Sama_1087 at 1315.150 kb on - strand, within Sama_1087 at 1315.150 kb on - strand, within Sama_1087 at 1315.150 kb on - strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.153 kb on + strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.154 kb on - strand, within Sama_1087 at 1315.157 kb on + strand, within Sama_1087 at 1315.158 kb on - strand, within Sama_1087 at 1315.158 kb on - strand, within Sama_1087 at 1315.158 kb on - strand, within Sama_1087 at 1315.168 kb on - strand, within Sama_1087 at 1315.192 kb on - strand, within Sama_1087 at 1315.192 kb on - strand, within Sama_1087 at 1315.192 kb on - strand, within Sama_1087 at 1315.209 kb on + strand, within Sama_1087 at 1315.209 kb on + strand, within Sama_1087 at 1315.209 kb on + strand, within Sama_1087 at 1315.209 kb on + strand, within Sama_1087 at 1315.210 kb on - strand, within Sama_1087 at 1315.210 kb on - strand, within Sama_1087 at 1315.210 kb on - strand, within Sama_1087 at 1315.232 kb on - strand, within Sama_1087 at 1315.305 kb on + strand, within Sama_1087 at 1315.311 kb on + strand, within Sama_1087 at 1315.312 kb on - strand, within Sama_1087 at 1315.312 kb on - strand, within Sama_1087 at 1315.434 kb on + strand, within Sama_1087 at 1315.434 kb on + strand, within Sama_1087 at 1315.434 kb on + strand, within Sama_1087 at 1315.434 kb on + strand, within Sama_1087 at 1315.434 kb on + strand, within Sama_1087 at 1315.435 kb on - strand, within Sama_1087 at 1315.435 kb on - strand, within Sama_1087 at 1315.436 kb on + strand, within Sama_1087 at 1315.436 kb on + strand, within Sama_1087 at 1315.436 kb on + strand, within Sama_1087 at 1315.436 kb on + strand, within Sama_1087 at 1315.437 kb on - strand, within Sama_1087 at 1315.437 kb on - strand, within Sama_1087 at 1315.437 kb on - strand, within Sama_1087 at 1315.437 kb on - strand, within Sama_1087 at 1315.437 kb on - strand, within Sama_1087 at 1315.437 kb on - strand, within Sama_1087 at 1315.461 kb on - strand, within Sama_1087 at 1315.461 kb on - strand, within Sama_1087 at 1315.477 kb on + strand, within Sama_1087 at 1315.477 kb on + strand, within Sama_1087 at 1315.478 kb on - strand, within Sama_1087 at 1315.478 kb on - strand, within Sama_1087 at 1315.488 kb on - strand, within Sama_1087 at 1315.488 kb on - strand, within Sama_1087 at 1315.571 kb on + strand, within Sama_1087 at 1315.572 kb on - strand, within Sama_1087 at 1315.589 kb on + strand at 1315.590 kb on - strand at 1315.641 kb on + strand at 1315.641 kb on + strand at 1315.642 kb on - strand at 1315.642 kb on - strand at 1315.659 kb on + strand at 1315.659 kb on + strand at 1315.659 kb on + strand at 1315.659 kb on + strand at 1315.659 kb on + strand at 1315.659 kb on + strand at 1315.660 kb on - strand at 1315.660 kb on - strand at 1315.660 kb on - strand at 1315.660 kb on - strand at 1315.660 kb on - strand at 1315.660 kb on - strand at 1315.660 kb on - strand at 1315.668 kb on - strand at 1315.668 kb on - strand at 1315.880 kb on - strand, within Sama_1088
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml remove 1,312,570 + Sama_1084 0.79 -1.0 1,312,570 + Sama_1084 0.79 -1.7 1,312,639 + Sama_1084 0.83 -1.1 1,312,639 + Sama_1084 0.83 -0.6 1,312,639 + Sama_1084 0.83 +0.0 1,312,639 + Sama_1084 0.83 -0.7 1,312,639 + Sama_1084 0.83 +0.7 1,312,639 + Sama_1084 0.83 -2.4 1,312,719 + Sama_1084 0.88 -0.4 1,312,719 + Sama_1084 0.88 +0.9 1,312,720 - Sama_1084 0.88 +1.6 1,312,749 + Sama_1084 0.90 -0.1 1,312,861 + -2.4 1,312,861 + +0.4 1,312,861 + -0.3 1,312,861 + +0.5 1,312,861 + -0.1 1,312,861 + +0.5 1,312,861 + -0.6 1,312,862 - -1.3 1,312,862 - -0.6 1,312,862 - -1.0 1,312,862 - -1.3 1,312,862 - -0.2 1,312,862 - -0.3 1,312,865 + -0.1 1,312,865 + +0.0 1,312,866 - +0.0 1,312,968 - +1.0 1,312,968 - -0.7 1,312,997 + +2.0 1,313,028 - -0.9 1,313,038 + +0.0 1,313,060 + -0.9 1,313,060 + -0.6 1,313,060 + -1.4 1,313,061 - -1.0 1,313,193 + Sama_1085 0.14 -0.8 1,313,193 + Sama_1085 0.14 +0.2 1,313,194 - Sama_1085 0.14 -0.4 1,313,194 - Sama_1085 0.14 +0.6 1,313,194 - Sama_1085 0.14 -1.7 1,313,221 - Sama_1085 0.31 -1.3 1,313,243 - Sama_1085 0.44 -0.3 1,313,243 - Sama_1085 0.44 -0.9 1,313,261 - Sama_1085 0.56 -2.4 1,313,319 + -2.0 1,313,319 + -0.6 1,313,380 + +0.4 1,313,402 - +0.0 1,313,416 - -0.6 1,313,460 - -1.3 1,313,460 - -0.4 1,313,460 - +0.3 1,313,478 + -1.7 1,313,479 - -1.1 1,313,566 + -0.3 1,313,566 + +1.3 1,313,725 - Sama_1086 0.14 -1.3 1,313,804 - Sama_1086 0.20 -1.6 1,313,858 - Sama_1086 0.24 -2.5 1,313,895 + Sama_1086 0.26 -1.6 1,313,895 + Sama_1086 0.26 -0.1 1,313,896 - Sama_1086 0.26 -1.1 1,313,896 - Sama_1086 0.26 -1.0 1,313,896 - Sama_1086 0.26 -1.1 1,314,004 + Sama_1086 0.34 -0.7 1,314,004 + Sama_1086 0.34 -1.1 1,314,005 - Sama_1086 0.34 -1.4 1,314,005 - Sama_1086 0.34 -0.1 1,314,070 + Sama_1086 0.39 -1.0 1,314,071 - Sama_1086 0.39 -1.0 1,314,178 + Sama_1086 0.47 +1.0 1,314,178 + Sama_1086 0.47 +1.8 1,314,178 + Sama_1086 0.47 +0.4 1,314,180 + Sama_1086 0.47 +0.7 1,314,181 - Sama_1086 0.47 +2.1 1,314,181 - Sama_1086 0.47 -0.5 1,314,181 - Sama_1086 0.47 -1.4 1,314,181 - Sama_1086 0.47 +0.6 1,314,181 - Sama_1086 0.47 +0.2 1,314,244 + Sama_1086 0.52 -1.5 1,314,244 + Sama_1086 0.52 +0.2 1,314,244 + Sama_1086 0.52 -2.0 1,314,244 + Sama_1086 0.52 -1.3 1,314,244 + Sama_1086 0.52 +0.4 1,314,244 + Sama_1086 0.52 -0.5 1,314,244 + Sama_1086 0.52 +0.7 1,314,245 - Sama_1086 0.52 +0.6 1,314,245 - Sama_1086 0.52 +1.9 1,314,245 - Sama_1086 0.52 -1.7 1,314,445 + Sama_1086 0.67 -0.8 1,314,446 - Sama_1086 0.67 +0.3 1,314,446 - Sama_1086 0.67 +0.1 1,314,446 - Sama_1086 0.67 +0.4 1,314,450 - Sama_1086 0.67 +0.3 1,314,450 - Sama_1086 0.67 +1.1 1,314,450 - Sama_1086 0.67 +0.2 1,314,450 - Sama_1086 0.67 +1.4 1,314,485 - Sama_1086 0.69 +0.0 1,314,587 + Sama_1086 0.77 +0.3 1,314,588 - Sama_1086 0.77 +0.4 1,314,588 - Sama_1086 0.77 +0.4 1,314,588 - Sama_1086 0.77 +0.2 1,314,588 - Sama_1086 0.77 -1.3 1,314,596 - Sama_1086 0.78 -1.4 1,314,652 + Sama_1086 0.82 +0.0 1,314,652 + Sama_1086 0.82 +1.0 1,314,652 + Sama_1086 0.82 -1.9 1,314,653 - Sama_1086 0.82 -3.5 1,314,739 + Sama_1086 0.88 +0.2 1,314,739 + Sama_1086 0.88 +0.6 1,314,739 + Sama_1086 0.88 -1.6 1,314,739 + Sama_1086 0.88 -1.9 1,314,739 + Sama_1086 0.88 -0.4 1,314,740 - Sama_1086 0.88 +0.0 1,314,740 - Sama_1086 0.88 -1.3 1,314,740 - Sama_1086 0.88 -0.5 1,314,740 - Sama_1086 0.88 -0.4 1,314,777 + -2.7 1,314,777 + -1.0 1,314,777 + +1.4 1,314,815 - +1.8 1,314,815 - -0.7 1,314,852 + +0.4 1,314,852 + +1.0 1,314,852 + -1.0 1,314,853 - +1.1 1,315,129 + Sama_1087 0.21 +1.5 1,315,149 + Sama_1087 0.24 -0.2 1,315,149 + Sama_1087 0.24 -0.4 1,315,149 + Sama_1087 0.24 -2.1 1,315,149 + Sama_1087 0.24 -1.8 1,315,149 + Sama_1087 0.24 +0.1 1,315,149 + Sama_1087 0.24 +0.0 1,315,149 + Sama_1087 0.24 -0.3 1,315,149 + Sama_1087 0.24 -1.7 1,315,149 + Sama_1087 0.24 -0.1 1,315,150 - Sama_1087 0.25 +3.4 1,315,150 - Sama_1087 0.25 -0.6 1,315,150 - Sama_1087 0.25 -0.6 1,315,150 - Sama_1087 0.25 +0.9 1,315,150 - Sama_1087 0.25 -0.0 1,315,153 + Sama_1087 0.25 -0.3 1,315,153 + Sama_1087 0.25 -0.6 1,315,153 + Sama_1087 0.25 -1.1 1,315,153 + Sama_1087 0.25 +0.0 1,315,153 + Sama_1087 0.25 -1.7 1,315,153 + Sama_1087 0.25 -0.6 1,315,153 + Sama_1087 0.25 -0.2 1,315,153 + Sama_1087 0.25 -2.5 1,315,153 + Sama_1087 0.25 -2.3 1,315,153 + Sama_1087 0.25 +0.0 1,315,153 + Sama_1087 0.25 +0.4 1,315,154 - Sama_1087 0.25 -1.1 1,315,154 - Sama_1087 0.25 -1.3 1,315,154 - Sama_1087 0.25 +0.4 1,315,154 - Sama_1087 0.25 -1.3 1,315,154 - Sama_1087 0.25 -1.7 1,315,154 - Sama_1087 0.25 +2.4 1,315,154 - Sama_1087 0.25 -0.2 1,315,154 - Sama_1087 0.25 +1.4 1,315,154 - Sama_1087 0.25 +3.6 1,315,154 - Sama_1087 0.25 -1.4 1,315,154 - Sama_1087 0.25 +0.1 1,315,154 - Sama_1087 0.25 -2.1 1,315,154 - Sama_1087 0.25 -0.3 1,315,154 - Sama_1087 0.25 -2.4 1,315,154 - Sama_1087 0.25 -0.9 1,315,154 - Sama_1087 0.25 -2.0 1,315,154 - Sama_1087 0.25 -0.3 1,315,154 - Sama_1087 0.25 -0.2 1,315,154 - Sama_1087 0.25 -0.9 1,315,154 - Sama_1087 0.25 +0.3 1,315,157 + Sama_1087 0.26 +1.0 1,315,158 - Sama_1087 0.26 -0.1 1,315,158 - Sama_1087 0.26 +1.3 1,315,158 - Sama_1087 0.26 +0.9 1,315,168 - Sama_1087 0.27 -0.7 1,315,192 - Sama_1087 0.31 -1.0 1,315,192 - Sama_1087 0.31 +0.9 1,315,192 - Sama_1087 0.31 +1.7 1,315,209 + Sama_1087 0.33 -0.3 1,315,209 + Sama_1087 0.33 +0.3 1,315,209 + Sama_1087 0.33 -0.6 1,315,209 + Sama_1087 0.33 -0.9 1,315,210 - Sama_1087 0.33 +0.1 1,315,210 - Sama_1087 0.33 -1.0 1,315,210 - Sama_1087 0.33 -0.6 1,315,232 - Sama_1087 0.37 -0.1 1,315,305 + Sama_1087 0.48 -1.1 1,315,311 + Sama_1087 0.49 -1.4 1,315,312 - Sama_1087 0.49 -1.1 1,315,312 - Sama_1087 0.49 -2.2 1,315,434 + Sama_1087 0.67 -0.8 1,315,434 + Sama_1087 0.67 +0.2 1,315,434 + Sama_1087 0.67 +1.3 1,315,434 + Sama_1087 0.67 -1.8 1,315,434 + Sama_1087 0.67 +0.2 1,315,435 - Sama_1087 0.67 -3.2 1,315,435 - Sama_1087 0.67 -0.6 1,315,436 + Sama_1087 0.67 -0.8 1,315,436 + Sama_1087 0.67 +0.2 1,315,436 + Sama_1087 0.67 -3.1 1,315,436 + Sama_1087 0.67 +0.6 1,315,437 - Sama_1087 0.67 -1.1 1,315,437 - Sama_1087 0.67 -0.6 1,315,437 - Sama_1087 0.67 -1.0 1,315,437 - Sama_1087 0.67 +0.7 1,315,437 - Sama_1087 0.67 +0.1 1,315,437 - Sama_1087 0.67 -1.0 1,315,461 - Sama_1087 0.71 -1.4 1,315,461 - Sama_1087 0.71 -0.3 1,315,477 + Sama_1087 0.73 -1.0 1,315,477 + Sama_1087 0.73 +0.8 1,315,478 - Sama_1087 0.74 -2.0 1,315,478 - Sama_1087 0.74 -0.8 1,315,488 - Sama_1087 0.75 -1.9 1,315,488 - Sama_1087 0.75 -0.3 1,315,571 + Sama_1087 0.87 +0.8 1,315,572 - Sama_1087 0.88 -0.6 1,315,589 + -2.8 1,315,590 - +2.2 1,315,641 + +0.6 1,315,641 + -0.8 1,315,642 - +0.8 1,315,642 - -0.4 1,315,659 + +0.1 1,315,659 + -3.4 1,315,659 + -2.0 1,315,659 + -0.3 1,315,659 + +0.2 1,315,659 + -1.9 1,315,660 - +1.9 1,315,660 - +0.5 1,315,660 - -0.3 1,315,660 - -1.4 1,315,660 - -1.5 1,315,660 - +0.3 1,315,660 - +1.0 1,315,668 - +1.2 1,315,668 - +0.5 1,315,880 - Sama_1088 0.34 -2.1
Or see this region's nucleotide sequence