Experiment: Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0060 and Sama_0061 are separated by 273 nucleotides Sama_0061 and Sama_0062 are separated by 219 nucleotides Sama_0062 and gpsA are separated by 6 nucleotides
Sama_0060: Sama_0060 - phosphoglyceromutase (RefSeq), at 65,149 to 66,690
_0060
Sama_0061: Sama_0061 - rhodanese-like protein (RefSeq), at 66,964 to 67,356
_0061
Sama_0062: Sama_0062 - preprotein translocase subunit SecB (RefSeq), at 67,576 to 68,055
_0062
Sama_0063: gpsA - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase (RefSeq), at 68,062 to 69,081
gpsA
Position (kb) 66
67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 66.715 kb on - strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.752 kb on + strand at 66.753 kb on - strand at 66.753 kb on - strand at 66.755 kb on + strand at 66.755 kb on + strand at 66.756 kb on - strand at 66.756 kb on - strand at 66.758 kb on + strand at 66.758 kb on + strand at 66.759 kb on - strand at 66.759 kb on - strand at 66.832 kb on + strand at 66.833 kb on - strand at 66.833 kb on - strand at 66.833 kb on - strand at 66.876 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.880 kb on + strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.881 kb on - strand at 66.882 kb on + strand at 66.882 kb on + strand at 66.883 kb on - strand at 66.883 kb on - strand at 66.883 kb on - strand at 66.907 kb on + strand at 66.908 kb on - strand at 66.913 kb on + strand at 66.914 kb on - strand at 66.914 kb on - strand at 66.924 kb on + strand at 66.924 kb on + strand at 66.937 kb on + strand at 66.937 kb on + strand at 66.937 kb on + strand at 66.938 kb on - strand at 66.938 kb on - strand at 67.008 kb on + strand, within Sama_0061 at 67.008 kb on + strand, within Sama_0061 at 67.008 kb on + strand, within Sama_0061 at 67.009 kb on - strand, within Sama_0061 at 67.009 kb on - strand, within Sama_0061 at 67.017 kb on + strand, within Sama_0061 at 67.017 kb on + strand, within Sama_0061 at 67.023 kb on + strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.024 kb on - strand, within Sama_0061 at 67.097 kb on + strand, within Sama_0061 at 67.098 kb on - strand, within Sama_0061 at 67.098 kb on - strand, within Sama_0061 at 67.111 kb on - strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.218 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.220 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.292 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.294 kb on + strand, within Sama_0061 at 67.311 kb on + strand, within Sama_0061 at 67.354 kb on + strand at 67.408 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.025 mg/ml remove 66,715 - +1.1 66,752 + -1.7 66,752 + -1.7 66,752 + -3.3 66,752 + -1.7 66,752 + -1.2 66,753 - +1.2 66,753 - +4.0 66,755 + -0.7 66,755 + -2.4 66,756 - +0.0 66,756 - -0.5 66,758 + +0.5 66,758 + +0.2 66,759 - -0.5 66,759 - +0.3 66,832 + +1.3 66,833 - +1.1 66,833 - -0.7 66,833 - -2.1 66,876 + -0.3 66,880 + -1.1 66,880 + -0.4 66,880 + +0.2 66,880 + +0.2 66,880 + +0.8 66,880 + +0.1 66,880 + -1.5 66,880 + +0.9 66,880 + -0.7 66,881 - +0.3 66,881 - -2.3 66,881 - +1.3 66,881 - -2.3 66,881 - -1.7 66,881 - -0.1 66,882 + -0.1 66,882 + +0.9 66,883 - -0.6 66,883 - -0.1 66,883 - +0.5 66,907 + +0.5 66,908 - +1.5 66,913 + -0.2 66,914 - -0.9 66,914 - -0.9 66,924 + -2.1 66,924 + -0.2 66,937 + -0.8 66,937 + -0.5 66,937 + +1.1 66,938 - -0.8 66,938 - +0.1 67,008 + Sama_0061 0.11 -1.1 67,008 + Sama_0061 0.11 +0.5 67,008 + Sama_0061 0.11 +0.9 67,009 - Sama_0061 0.11 -0.5 67,009 - Sama_0061 0.11 -2.5 67,017 + Sama_0061 0.13 -0.4 67,017 + Sama_0061 0.13 -0.1 67,023 + Sama_0061 0.15 +1.3 67,024 - Sama_0061 0.15 +1.1 67,024 - Sama_0061 0.15 -1.7 67,024 - Sama_0061 0.15 -0.7 67,024 - Sama_0061 0.15 -1.1 67,097 + Sama_0061 0.34 -0.6 67,098 - Sama_0061 0.34 +0.3 67,098 - Sama_0061 0.34 -0.7 67,111 - Sama_0061 0.37 -0.3 67,218 + Sama_0061 0.65 +0.4 67,218 + Sama_0061 0.65 -0.6 67,218 + Sama_0061 0.65 +0.0 67,218 + Sama_0061 0.65 -0.6 67,218 + Sama_0061 0.65 -0.1 67,218 + Sama_0061 0.65 -0.5 67,218 + Sama_0061 0.65 -0.3 67,218 + Sama_0061 0.65 -1.3 67,218 + Sama_0061 0.65 -2.3 67,220 + Sama_0061 0.65 +0.6 67,220 + Sama_0061 0.65 -0.5 67,220 + Sama_0061 0.65 +0.1 67,220 + Sama_0061 0.65 +0.0 67,220 + Sama_0061 0.65 -0.7 67,220 + Sama_0061 0.65 +1.4 67,220 + Sama_0061 0.65 +0.0 67,292 + Sama_0061 0.83 +2.0 67,292 + Sama_0061 0.83 -0.6 67,292 + Sama_0061 0.83 -1.1 67,292 + Sama_0061 0.83 -1.5 67,292 + Sama_0061 0.83 +0.6 67,292 + Sama_0061 0.83 -0.6 67,294 + Sama_0061 0.84 -1.1 67,294 + Sama_0061 0.84 +1.1 67,294 + Sama_0061 0.84 -0.3 67,294 + Sama_0061 0.84 +2.4 67,294 + Sama_0061 0.84 +0.0 67,311 + Sama_0061 0.88 +0.2 67,354 + -2.6 67,408 + +0.6
Or see this region's nucleotide sequence