Experiment: m.b. Cholin acetate 76.57 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_488 and HP15_490 are separated by 98 nucleotides HP15_490 and HP15_489 are separated by 15 nucleotides HP15_489 and HP15_491 are separated by 77 nucleotides HP15_491 and HP15_492 overlap by 4 nucleotides
HP15_488: HP15_488 - ABC transporter ATP-binding protein-like protein, at 503,193 to 504,854
_488
HP15_490: HP15_490 - hypothetical protein, at 504,953 to 505,237
_490
HP15_489: HP15_489 - glycine hydroxymethyltransferase, at 505,253 to 506,506
_489
HP15_491: HP15_491 - transcriptional repressor NrdR, at 506,584 to 507,102
_491
HP15_492: HP15_492 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase, at 507,099 to 508,214
_492
Position (kb) 505
506
507 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 504.308 kb on + strand, within HP15_488 at 504.333 kb on + strand, within HP15_488 at 504.333 kb on + strand, within HP15_488 at 504.346 kb on - strand, within HP15_488 at 504.346 kb on - strand, within HP15_488 at 504.369 kb on - strand, within HP15_488 at 504.543 kb on - strand, within HP15_488 at 504.703 kb on + strand at 504.713 kb on + strand at 504.835 kb on + strand at 504.843 kb on - strand at 504.913 kb on + strand at 504.921 kb on - strand at 504.944 kb on - strand at 504.997 kb on + strand, within HP15_490 at 505.005 kb on - strand, within HP15_490 at 505.005 kb on - strand, within HP15_490 at 505.019 kb on + strand, within HP15_490 at 505.027 kb on + strand, within HP15_490 at 505.042 kb on + strand, within HP15_490 at 505.050 kb on - strand, within HP15_490 at 505.100 kb on + strand, within HP15_490 at 505.143 kb on - strand, within HP15_490 at 506.916 kb on + strand, within HP15_491
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 76.57 mM remove 504,308 + HP15_488 0.67 -1.1 504,333 + HP15_488 0.69 +0.1 504,333 + HP15_488 0.69 +0.4 504,346 - HP15_488 0.69 +0.1 504,346 - HP15_488 0.69 +0.2 504,369 - HP15_488 0.71 -1.1 504,543 - HP15_488 0.81 -0.6 504,703 + -0.2 504,713 + +0.7 504,835 + +0.4 504,843 - +0.2 504,913 + -0.3 504,921 - +0.0 504,944 - -0.0 504,997 + HP15_490 0.15 +0.5 505,005 - HP15_490 0.18 -5.3 505,005 - HP15_490 0.18 -0.3 505,019 + HP15_490 0.23 -0.7 505,027 + HP15_490 0.26 -0.0 505,042 + HP15_490 0.31 -0.3 505,050 - HP15_490 0.34 -0.2 505,100 + HP15_490 0.52 -0.3 505,143 - HP15_490 0.67 -0.9 506,916 + HP15_491 0.64 -1.6
Or see this region's nucleotide sequence