Experiment: m.b. Cholin acetate 76.57 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1296 and HP15_1297 overlap by 4 nucleotides HP15_1297 and HP15_1298 are separated by 72 nucleotides HP15_1298 and HP15_1299 overlap by 4 nucleotides
HP15_1296: HP15_1296 - valine-pyruvate transaminase, at 1,356,526 to 1,357,803
_1296
HP15_1297: HP15_1297 - conserved hypothetical protein, secreted, at 1,357,800 to 1,358,552
_1297
HP15_1298: HP15_1298 - phosphoribosylglycinamide formyltransferase, at 1,358,625 to 1,359,287
_1298
HP15_1299: HP15_1299 - phosphoribosylaminoimidazole synthetase, at 1,359,284 to 1,360,414
_1299
Position (kb)
1358
1359
1360 Strain fitness (log2 ratio)
-2
-1
0
1 at 1357.764 kb on + strand at 1357.764 kb on + strand at 1357.764 kb on + strand at 1357.764 kb on + strand at 1357.772 kb on - strand at 1357.772 kb on - strand at 1357.772 kb on - strand at 1357.804 kb on + strand at 1358.248 kb on + strand, within HP15_1297 at 1358.256 kb on - strand, within HP15_1297 at 1358.338 kb on + strand, within HP15_1297 at 1358.599 kb on - strand at 1358.733 kb on - strand, within HP15_1298 at 1358.902 kb on + strand, within HP15_1298 at 1358.939 kb on + strand, within HP15_1298 at 1359.112 kb on + strand, within HP15_1298 at 1359.150 kb on + strand, within HP15_1298 at 1359.278 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 76.57 mM remove 1,357,764 + -0.2 1,357,764 + +1.1 1,357,764 + -0.1 1,357,764 + +0.3 1,357,772 - -0.2 1,357,772 - +0.2 1,357,772 - +0.6 1,357,804 + +0.3 1,358,248 + HP15_1297 0.59 +0.8 1,358,256 - HP15_1297 0.61 +1.2 1,358,338 + HP15_1297 0.71 +0.3 1,358,599 - +0.6 1,358,733 - HP15_1298 0.16 -0.5 1,358,902 + HP15_1298 0.42 -2.0 1,358,939 + HP15_1298 0.47 -1.4 1,359,112 + HP15_1298 0.73 -1.3 1,359,150 + HP15_1298 0.79 -1.2 1,359,278 - -1.6
Or see this region's nucleotide sequence