Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0193 and Psest_0194 are separated by 178 nucleotides Psest_0194 and Psest_0195 are separated by 110 nucleotides Psest_0195 and Psest_0196 are separated by 390 nucleotides
Psest_0193: Psest_0193 - conserved hypothetical protein, at 189,910 to 190,341
_0193
Psest_0194: Psest_0194 - hypothetical protein, at 190,520 to 190,756
_0194
Psest_0195: Psest_0195 - Small integral membrane protein, at 190,867 to 191,037
_0195
Psest_0196: Psest_0196 - Uncharacterized membrane protein, required for spore maturation in B.subtilis., at 191,428 to 192,657
_0196
Position (kb)
190
191
192 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 189.974 kb on - strand, within Psest_0193 at 190.138 kb on + strand, within Psest_0193 at 190.474 kb on - strand at 190.505 kb on + strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.506 kb on - strand at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.661 kb on + strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.662 kb on - strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.675 kb on + strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand at 190.676 kb on - strand, within Psest_0194 at 190.676 kb on - strand, within Psest_0194 at 190.881 kb on + strand at 190.881 kb on + strand at 190.881 kb on + strand at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.884 kb on + strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.885 kb on - strand, within Psest_0195 at 190.974 kb on - strand, within Psest_0195 at 190.974 kb on - strand, within Psest_0195 at 191.089 kb on + strand at 191.089 kb on + strand at 191.089 kb on + strand at 191.089 kb on + strand at 191.089 kb on + strand at 191.090 kb on - strand at 191.090 kb on - strand at 191.090 kb on - strand at 191.222 kb on + strand at 191.222 kb on + strand at 191.222 kb on + strand at 191.324 kb on - strand at 191.358 kb on - strand at 191.528 kb on + strand at 191.528 kb on + strand at 191.528 kb on + strand at 191.528 kb on + strand at 191.529 kb on - strand at 191.529 kb on - strand at 191.529 kb on - strand at 191.865 kb on + strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.866 kb on - strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.905 kb on + strand, within Psest_0196 at 191.906 kb on - strand, within Psest_0196
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 189,974 - Psest_0193 0.15 +0.0 190,138 + Psest_0193 0.53 -1.1 190,474 - -1.3 190,505 + +1.0 190,506 - -0.6 190,506 - +0.8 190,506 - +0.3 190,506 - -0.1 190,506 - +0.9 190,506 - -0.6 190,506 - +1.0 190,661 + Psest_0194 0.59 +0.4 190,661 + Psest_0194 0.59 +0.0 190,661 + Psest_0194 0.59 +0.4 190,661 + Psest_0194 0.59 +0.6 190,661 + Psest_0194 0.59 +0.8 190,661 + Psest_0194 0.59 +0.3 190,661 + Psest_0194 0.59 +0.3 190,661 + Psest_0194 0.59 +0.3 190,661 + Psest_0194 0.59 -1.5 190,661 + Psest_0194 0.59 -0.7 190,661 + Psest_0194 0.59 +0.7 190,661 + Psest_0194 0.59 +0.1 190,662 - Psest_0194 0.60 -0.4 190,662 - Psest_0194 0.60 -1.0 190,662 - Psest_0194 0.60 -0.3 190,662 - Psest_0194 0.60 -1.3 190,662 - Psest_0194 0.60 -1.5 190,662 - Psest_0194 0.60 -1.1 190,662 - Psest_0194 0.60 +1.2 190,662 - Psest_0194 0.60 +0.3 190,662 - Psest_0194 0.60 +0.2 190,675 + Psest_0194 0.65 +0.1 190,675 + Psest_0194 0.65 -0.1 190,675 + Psest_0194 0.65 -0.5 190,675 + Psest_0194 0.65 -0.1 190,675 + Psest_0194 0.65 +0.0 190,675 + Psest_0194 0.65 +0.1 190,676 - Psest_0194 0.66 +1.0 190,676 - Psest_0194 0.66 +0.1 190,676 - Psest_0194 0.66 -0.4 190,676 - -1.3 190,676 - Psest_0194 0.66 -2.1 190,676 - Psest_0194 0.66 -0.6 190,881 + -0.2 190,881 + -0.4 190,881 + +0.4 190,884 + Psest_0195 0.10 +1.7 190,884 + Psest_0195 0.10 -0.4 190,884 + Psest_0195 0.10 +0.0 190,884 + Psest_0195 0.10 +0.2 190,884 + Psest_0195 0.10 -0.9 190,884 + Psest_0195 0.10 +0.5 190,884 + Psest_0195 0.10 -0.2 190,884 + Psest_0195 0.10 -0.7 190,884 + Psest_0195 0.10 -0.6 190,884 + Psest_0195 0.10 -0.8 190,884 + Psest_0195 0.10 +0.6 190,884 + Psest_0195 0.10 -0.3 190,884 + Psest_0195 0.10 -1.0 190,884 + Psest_0195 0.10 -1.4 190,884 + Psest_0195 0.10 -0.5 190,884 + Psest_0195 0.10 -0.8 190,885 - Psest_0195 0.11 -0.2 190,885 - Psest_0195 0.11 -2.0 190,885 - Psest_0195 0.11 +1.5 190,885 - Psest_0195 0.11 -1.7 190,885 - Psest_0195 0.11 -0.7 190,885 - Psest_0195 0.11 -0.8 190,885 - Psest_0195 0.11 -1.0 190,885 - Psest_0195 0.11 -0.5 190,885 - Psest_0195 0.11 -0.7 190,885 - Psest_0195 0.11 -3.2 190,974 - Psest_0195 0.63 -0.3 190,974 - Psest_0195 0.63 +0.2 191,089 + +0.2 191,089 + +0.8 191,089 + -0.5 191,089 + -0.2 191,089 + -0.0 191,090 - -0.3 191,090 - +1.5 191,090 - -1.1 191,222 + +0.2 191,222 + +1.0 191,222 + +1.7 191,324 - +0.0 191,358 - +0.9 191,528 + +0.9 191,528 + -0.5 191,528 + +0.6 191,528 + -0.2 191,529 - +0.2 191,529 - +0.4 191,529 - -0.4 191,865 + Psest_0196 0.36 -0.3 191,866 - Psest_0196 0.36 -1.4 191,866 - Psest_0196 0.36 +0.3 191,866 - Psest_0196 0.36 -0.1 191,866 - Psest_0196 0.36 -0.1 191,905 + Psest_0196 0.39 -0.1 191,905 + Psest_0196 0.39 +0.2 191,905 + Psest_0196 0.39 -0.7 191,905 + Psest_0196 0.39 +0.1 191,905 + Psest_0196 0.39 +0.6 191,906 - Psest_0196 0.39 +0.6
Or see this region's nucleotide sequence