Experiment: Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1427 and Psest_1428 are separated by 13 nucleotides Psest_1428 and Psest_1429 are separated by 80 nucleotides
Psest_1427: Psest_1427 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 1,535,817 to 1,536,839
_1427
Psest_1428: Psest_1428 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 1,536,853 to 1,537,941
_1428
Psest_1429: Psest_1429 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit, at 1,538,022 to 1,539,011
_1429
Position (kb)
1536
1537
1538 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1536.008 kb on - strand, within Psest_1427 at 1536.008 kb on - strand, within Psest_1427 at 1536.232 kb on + strand, within Psest_1427 at 1536.232 kb on + strand, within Psest_1427 at 1536.232 kb on + strand, within Psest_1427 at 1536.232 kb on + strand, within Psest_1427 at 1536.232 kb on + strand, within Psest_1427 at 1536.233 kb on - strand, within Psest_1427 at 1536.233 kb on - strand, within Psest_1427 at 1536.233 kb on - strand, within Psest_1427 at 1536.313 kb on + strand, within Psest_1427 at 1536.314 kb on - strand, within Psest_1427 at 1536.314 kb on - strand, within Psest_1427 at 1536.314 kb on - strand, within Psest_1427 at 1536.367 kb on + strand, within Psest_1427 at 1536.421 kb on + strand, within Psest_1427 at 1536.514 kb on + strand, within Psest_1427 at 1536.515 kb on - strand, within Psest_1427 at 1536.515 kb on - strand, within Psest_1427 at 1536.515 kb on - strand, within Psest_1427 at 1536.515 kb on - strand, within Psest_1427 at 1536.538 kb on + strand, within Psest_1427 at 1536.538 kb on + strand, within Psest_1427 at 1536.538 kb on + strand, within Psest_1427 at 1536.539 kb on - strand, within Psest_1427 at 1536.539 kb on - strand, within Psest_1427 at 1536.539 kb on - strand, within Psest_1427 at 1536.688 kb on + strand, within Psest_1427 at 1536.688 kb on + strand, within Psest_1427 at 1536.689 kb on - strand, within Psest_1427 at 1536.825 kb on + strand at 1536.826 kb on - strand at 1536.826 kb on - strand at 1536.826 kb on - strand at 1537.394 kb on - strand, within Psest_1428 at 1537.394 kb on - strand, within Psest_1428 at 1537.394 kb on - strand, within Psest_1428 at 1537.555 kb on + strand, within Psest_1428 at 1537.589 kb on + strand, within Psest_1428 at 1537.589 kb on + strand, within Psest_1428 at 1537.791 kb on - strand, within Psest_1428 at 1538.231 kb on - strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.681 kb on + strand, within Psest_1429 at 1538.682 kb on - strand, within Psest_1429 at 1538.682 kb on - strand, within Psest_1429 at 1538.682 kb on - strand, within Psest_1429 at 1538.682 kb on - strand, within Psest_1429 at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.923 kb on + strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand at 1538.924 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dimethyl Sulfoxide 5 vol% remove 1,536,008 - Psest_1427 0.19 -0.1 1,536,008 - Psest_1427 0.19 +2.0 1,536,232 + Psest_1427 0.41 +1.8 1,536,232 + Psest_1427 0.41 -0.6 1,536,232 + Psest_1427 0.41 -0.1 1,536,232 + Psest_1427 0.41 +0.1 1,536,232 + Psest_1427 0.41 +0.7 1,536,233 - Psest_1427 0.41 +0.4 1,536,233 - Psest_1427 0.41 -0.9 1,536,233 - Psest_1427 0.41 +0.9 1,536,313 + Psest_1427 0.48 +0.1 1,536,314 - Psest_1427 0.49 +1.0 1,536,314 - Psest_1427 0.49 +1.0 1,536,314 - Psest_1427 0.49 +0.0 1,536,367 + Psest_1427 0.54 -0.7 1,536,421 + Psest_1427 0.59 -0.2 1,536,514 + Psest_1427 0.68 -0.8 1,536,515 - Psest_1427 0.68 +0.2 1,536,515 - Psest_1427 0.68 -0.1 1,536,515 - Psest_1427 0.68 -0.4 1,536,515 - Psest_1427 0.68 -0.6 1,536,538 + Psest_1427 0.70 -2.2 1,536,538 + Psest_1427 0.70 +0.4 1,536,538 + Psest_1427 0.70 +0.7 1,536,539 - Psest_1427 0.71 +0.0 1,536,539 - Psest_1427 0.71 +1.0 1,536,539 - Psest_1427 0.71 -1.0 1,536,688 + Psest_1427 0.85 -0.1 1,536,688 + Psest_1427 0.85 -0.6 1,536,689 - Psest_1427 0.85 +0.8 1,536,825 + +0.3 1,536,826 - +1.0 1,536,826 - +1.2 1,536,826 - +0.1 1,537,394 - Psest_1428 0.50 +1.4 1,537,394 - Psest_1428 0.50 -0.7 1,537,394 - Psest_1428 0.50 +0.3 1,537,555 + Psest_1428 0.64 -0.5 1,537,589 + Psest_1428 0.68 -0.2 1,537,589 + Psest_1428 0.68 -0.7 1,537,791 - Psest_1428 0.86 -1.0 1,538,231 - Psest_1429 0.21 -0.2 1,538,681 + Psest_1429 0.67 +0.4 1,538,681 + Psest_1429 0.67 -0.7 1,538,681 + Psest_1429 0.67 -0.8 1,538,681 + Psest_1429 0.67 +0.8 1,538,681 + Psest_1429 0.67 -2.0 1,538,681 + Psest_1429 0.67 +0.4 1,538,681 + Psest_1429 0.67 -1.1 1,538,681 + Psest_1429 0.67 -0.6 1,538,682 - Psest_1429 0.67 -1.0 1,538,682 - Psest_1429 0.67 -0.3 1,538,682 - Psest_1429 0.67 -1.2 1,538,682 - Psest_1429 0.67 +1.0 1,538,923 + -1.3 1,538,923 + -0.0 1,538,923 + +0.3 1,538,923 + +0.3 1,538,923 + +1.9 1,538,923 + -0.1 1,538,923 + -0.4 1,538,923 + -0.9 1,538,923 + +0.4 1,538,924 - +0.3 1,538,924 - -0.4 1,538,924 - -0.3 1,538,924 - +0.2 1,538,924 - -0.2 1,538,924 - -0.2 1,538,924 - -0.1 1,538,924 - -1.0 1,538,924 - -0.2 1,538,924 - +0.1 1,538,924 - -0.6
Or see this region's nucleotide sequence