Experiment: m.b. Cholin acetate 153.15 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1274 and HP15_1275 are separated by 226 nucleotides HP15_1275 and HP15_1276 are separated by 6 nucleotides HP15_1276 and HP15_1277 are separated by 26 nucleotides HP15_1277 and HP15_1278 overlap by 4 nucleotides
HP15_1274: HP15_1274 - phosphopyruvate hydratase, at 1,335,850 to 1,337,145
_1274
HP15_1275: HP15_1275 - septum formation initiator, at 1,337,372 to 1,337,623
_1275
HP15_1276: HP15_1276 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 1,337,630 to 1,338,331
_1276
HP15_1277: HP15_1277 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 1,338,358 to 1,338,837
_1277
HP15_1278: HP15_1278 - pseudouridylate synthase, at 1,338,834 to 1,339,883
_1278
Position (kb)
1337
1338
1339 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1337.181 kb on - strand at 1337.290 kb on + strand at 1337.298 kb on - strand at 1337.298 kb on - strand at 1337.298 kb on - strand at 1337.626 kb on + strand at 1338.333 kb on - strand at 1338.882 kb on - strand at 1338.901 kb on - strand at 1338.932 kb on + strand at 1338.955 kb on - strand, within HP15_1278 at 1339.262 kb on - strand, within HP15_1278 at 1339.267 kb on + strand, within HP15_1278 at 1339.293 kb on - strand, within HP15_1278 at 1339.295 kb on + strand, within HP15_1278
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cholin acetate 153.15 mM remove 1,337,181 - +0.2 1,337,290 + +0.9 1,337,298 - -2.7 1,337,298 - -1.3 1,337,298 - -1.2 1,337,626 + +0.6 1,338,333 - +1.9 1,338,882 - +0.6 1,338,901 - +1.0 1,338,932 + -0.8 1,338,955 - HP15_1278 0.12 +0.2 1,339,262 - HP15_1278 0.41 -0.1 1,339,267 + HP15_1278 0.41 +0.2 1,339,293 - HP15_1278 0.44 -0.4 1,339,295 + HP15_1278 0.44 +0.3
Or see this region's nucleotide sequence