Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1013 and Psest_1014 are separated by 199 nucleotides Psest_1014 and Psest_1015 are separated by 76 nucleotides Psest_1015 and Psest_1016 overlap by 4 nucleotides
Psest_1013: Psest_1013 - phosphoribosylamine--glycine ligase, at 1,098,615 to 1,099,907
_1013
Psest_1014: Psest_1014 - phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 1,100,107 to 1,101,711
_1014
Psest_1015: Psest_1015 - Factor for inversion stimulation Fis, transcriptional activator, at 1,101,788 to 1,102,108
_1015
Psest_1016: Psest_1016 - putative TIM-barrel protein, nifR3 family, at 1,102,105 to 1,103,118
_1016
Position (kb)
1100
1101
1102 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1099.114 kb on + strand, within Psest_1013 at 1099.114 kb on + strand, within Psest_1013 at 1099.115 kb on - strand, within Psest_1013 at 1099.179 kb on - strand, within Psest_1013 at 1099.213 kb on + strand, within Psest_1013 at 1099.214 kb on - strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.510 kb on + strand, within Psest_1013 at 1099.547 kb on - strand, within Psest_1013 at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.899 kb on + strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.900 kb on - strand at 1099.972 kb on + strand at 1099.979 kb on - strand at 1100.001 kb on + strand at 1100.001 kb on + strand at 1100.001 kb on + strand at 1100.002 kb on - strand at 1100.002 kb on - strand at 1100.002 kb on - strand at 1100.108 kb on + strand at 1101.204 kb on - strand, within Psest_1014 at 1102.083 kb on - strand at 1102.254 kb on - strand, within Psest_1016 at 1102.302 kb on - strand, within Psest_1016 at 1102.302 kb on - strand, within Psest_1016 at 1102.392 kb on - strand, within Psest_1016 at 1102.392 kb on - strand, within Psest_1016 at 1102.563 kb on - strand, within Psest_1016 at 1102.563 kb on - strand, within Psest_1016 at 1102.580 kb on + strand, within Psest_1016 at 1102.581 kb on - strand, within Psest_1016 at 1102.581 kb on - strand, within Psest_1016
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,099,114 + Psest_1013 0.39 +2.5 1,099,114 + Psest_1013 0.39 +2.4 1,099,115 - Psest_1013 0.39 +2.3 1,099,179 - Psest_1013 0.44 +2.4 1,099,213 + Psest_1013 0.46 +3.2 1,099,214 - Psest_1013 0.46 +1.5 1,099,510 + Psest_1013 0.69 -0.2 1,099,510 + Psest_1013 0.69 +2.6 1,099,510 + Psest_1013 0.69 +1.7 1,099,510 + Psest_1013 0.69 +0.5 1,099,510 + Psest_1013 0.69 +2.9 1,099,510 + Psest_1013 0.69 +2.7 1,099,547 - Psest_1013 0.72 +1.9 1,099,899 + +2.0 1,099,899 + +1.4 1,099,899 + +1.8 1,099,899 + +2.7 1,099,899 + +0.2 1,099,900 - +2.0 1,099,900 - +1.2 1,099,900 - +1.4 1,099,900 - +1.6 1,099,900 - +1.6 1,099,900 - +1.7 1,099,972 + +2.0 1,099,979 - +0.4 1,100,001 + +1.0 1,100,001 + -2.7 1,100,001 + +2.1 1,100,002 - -1.4 1,100,002 - -0.2 1,100,002 - +0.9 1,100,108 + -2.5 1,101,204 - Psest_1014 0.68 +0.4 1,102,083 - -0.1 1,102,254 - Psest_1016 0.15 +0.0 1,102,302 - Psest_1016 0.19 +1.0 1,102,302 - Psest_1016 0.19 +1.8 1,102,392 - Psest_1016 0.28 +1.2 1,102,392 - Psest_1016 0.28 +0.7 1,102,563 - Psest_1016 0.45 +0.4 1,102,563 - Psest_1016 0.45 -0.6 1,102,580 + Psest_1016 0.47 +2.5 1,102,581 - Psest_1016 0.47 +1.5 1,102,581 - Psest_1016 0.47 +1.5
Or see this region's nucleotide sequence