Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1004 and Psest_1005 overlap by 1 nucleotides Psest_1005 and Psest_1006 are separated by 263 nucleotides Psest_1006 and Psest_1007 are separated by 115 nucleotides
Psest_1004: Psest_1004 - Aspartate/tyrosine/aromatic aminotransferase, at 1,088,935 to 1,090,107
_1004
Psest_1005: Psest_1005 - sugar fermentation stimulation protein, at 1,090,107 to 1,090,814
_1005
Psest_1006: Psest_1006 - dTDP-4-dehydrorhamnose reductase, at 1,091,078 to 1,091,998
_1006
Psest_1007: Psest_1007 - Predicted permeases, at 1,092,114 to 1,093,013
_1007
Position (kb)
1091
1092 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1090.152 kb on + strand at 1090.443 kb on + strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.701 kb on + strand, within Psest_1005 at 1090.943 kb on - strand at 1091.255 kb on + strand, within Psest_1006 at 1091.256 kb on - strand, within Psest_1006 at 1091.256 kb on - strand, within Psest_1006 at 1091.297 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.368 kb on + strand, within Psest_1006 at 1091.369 kb on - strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.381 kb on + strand, within Psest_1006 at 1091.382 kb on - strand, within Psest_1006 at 1091.382 kb on - strand, within Psest_1006 at 1091.411 kb on + strand, within Psest_1006 at 1091.411 kb on + strand, within Psest_1006 at 1091.412 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.448 kb on - strand, within Psest_1006 at 1091.529 kb on - strand, within Psest_1006 at 1091.705 kb on + strand, within Psest_1006 at 1091.706 kb on - strand, within Psest_1006 at 1091.711 kb on + strand, within Psest_1006 at 1091.711 kb on + strand, within Psest_1006 at 1091.712 kb on - strand, within Psest_1006 at 1091.739 kb on - strand, within Psest_1006 at 1091.791 kb on - strand, within Psest_1006 at 1092.053 kb on + strand at 1092.054 kb on - strand at 1092.054 kb on - strand at 1092.054 kb on - strand at 1092.166 kb on - strand at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.316 kb on - strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.416 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.474 kb on + strand, within Psest_1007 at 1092.475 kb on - strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.531 kb on + strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.532 kb on - strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.559 kb on + strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007 at 1092.560 kb on - strand, within Psest_1007
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 1,090,152 + +0.6 1,090,443 + Psest_1005 0.47 -0.6 1,090,444 - Psest_1005 0.48 +2.5 1,090,444 - Psest_1005 0.48 -2.9 1,090,444 - Psest_1005 0.48 +1.8 1,090,444 - Psest_1005 0.48 -1.1 1,090,444 - Psest_1005 0.48 -0.7 1,090,642 - Psest_1005 0.76 -1.5 1,090,642 - Psest_1005 0.76 +1.5 1,090,699 + Psest_1005 0.84 +1.1 1,090,699 + Psest_1005 0.84 -1.6 1,090,700 - Psest_1005 0.84 +1.5 1,090,700 - Psest_1005 0.84 +0.4 1,090,701 + Psest_1005 0.84 -2.6 1,090,943 - +1.1 1,091,255 + Psest_1006 0.19 -1.7 1,091,256 - Psest_1006 0.19 -1.2 1,091,256 - Psest_1006 0.19 -2.9 1,091,297 + Psest_1006 0.24 +0.1 1,091,368 + Psest_1006 0.31 +1.2 1,091,368 + Psest_1006 0.31 -0.9 1,091,368 + Psest_1006 0.31 +0.2 1,091,369 - Psest_1006 0.32 +0.1 1,091,381 + Psest_1006 0.33 -0.3 1,091,381 + Psest_1006 0.33 -1.0 1,091,381 + Psest_1006 0.33 +1.5 1,091,382 - Psest_1006 0.33 +0.8 1,091,382 - Psest_1006 0.33 -0.8 1,091,411 + Psest_1006 0.36 +1.5 1,091,411 + Psest_1006 0.36 -2.4 1,091,412 - Psest_1006 0.36 -2.6 1,091,448 - Psest_1006 0.40 -0.2 1,091,448 - Psest_1006 0.40 +0.7 1,091,448 - Psest_1006 0.40 -3.3 1,091,448 - Psest_1006 0.40 -2.6 1,091,529 - Psest_1006 0.49 +2.2 1,091,705 + Psest_1006 0.68 -0.3 1,091,706 - Psest_1006 0.68 +0.7 1,091,711 + Psest_1006 0.69 -1.2 1,091,711 + Psest_1006 0.69 -1.4 1,091,712 - Psest_1006 0.69 -2.3 1,091,739 - Psest_1006 0.72 +2.1 1,091,791 - Psest_1006 0.77 -2.3 1,092,053 + -1.3 1,092,054 - -0.3 1,092,054 - -2.2 1,092,054 - -2.2 1,092,166 - -0.6 1,092,316 - Psest_1007 0.22 +1.9 1,092,316 - Psest_1007 0.22 -2.4 1,092,316 - Psest_1007 0.22 -2.2 1,092,416 + Psest_1007 0.34 +1.3 1,092,416 + Psest_1007 0.34 -1.3 1,092,416 + Psest_1007 0.34 +0.9 1,092,474 + Psest_1007 0.40 +1.5 1,092,474 + Psest_1007 0.40 -1.2 1,092,474 + Psest_1007 0.40 +2.1 1,092,474 + Psest_1007 0.40 +1.6 1,092,474 + Psest_1007 0.40 +0.8 1,092,475 - Psest_1007 0.40 +1.0 1,092,531 + Psest_1007 0.46 +0.8 1,092,531 + Psest_1007 0.46 -0.2 1,092,532 - Psest_1007 0.46 +1.3 1,092,532 - Psest_1007 0.46 +1.4 1,092,532 - Psest_1007 0.46 -2.1 1,092,532 - Psest_1007 0.46 -3.6 1,092,532 - Psest_1007 0.46 -1.3 1,092,532 - Psest_1007 0.46 -3.4 1,092,532 - Psest_1007 0.46 +3.8 1,092,532 - Psest_1007 0.46 -3.6 1,092,559 + Psest_1007 0.49 -2.7 1,092,559 + Psest_1007 0.49 -1.2 1,092,559 + Psest_1007 0.49 -0.4 1,092,560 - Psest_1007 0.50 +1.0 1,092,560 - Psest_1007 0.50 +1.5
Or see this region's nucleotide sequence