Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0007 and Psest_0008 are separated by 50 nucleotides Psest_0008 and Psest_0009 overlap by 4 nucleotides Psest_0009 and Psest_0010 overlap by 32 nucleotides
Psest_0007: Psest_0007 - diguanylate cyclase (GGDEF) domain, at 8,642 to 10,012
_0007
Psest_0008: Psest_0008 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 10,063 to 10,812
_0008
Psest_0009: Psest_0009 - histidinol-phosphate phosphatase family domain/HAD-superfamily hydrolase, subfamily IIIA, at 10,809 to 11,387
_0009
Psest_0010: Psest_0010 - glycyl-tRNA synthetase, tetrameric type, beta subunit, at 11,356 to 13,410
_0010
Position (kb) 10
11
12 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 9.883 kb on - strand at 9.883 kb on - strand at 10.001 kb on + strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.002 kb on - strand at 10.013 kb on - strand at 10.828 kb on - strand at 10.828 kb on - strand at 10.828 kb on - strand at 11.358 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 9,883 - -3.3 9,883 - +2.1 10,001 + +1.2 10,002 - +0.1 10,002 - -1.0 10,002 - +0.6 10,002 - -3.2 10,002 - -0.3 10,013 - -0.2 10,828 - -0.5 10,828 - -3.0 10,828 - -1.4 11,358 - +0.5
Or see this region's nucleotide sequence