Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0846

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0844 and Psest_0845 are separated by 16 nucleotidesPsest_0845 and Psest_0846 overlap by 1 nucleotidesPsest_0846 and Psest_0847 are separated by 270 nucleotides Psest_0844: Psest_0844 - High-affinity Fe2+/Pb2+ permease, at 914,601 to 915,458 _0844 Psest_0845: Psest_0845 - hypothetical protein, at 915,475 to 915,813 _0845 Psest_0846: Psest_0846 - Uncharacterized protein probably involved in high-affinity Fe2+ transport, at 915,813 to 916,346 _0846 Psest_0847: Psest_0847 - drug resistance transporter, EmrB/QacA subfamily, at 916,617 to 918,044 _0847 Position (kb) 915 916 917Strain fitness (log2 ratio) -2 -1 0 1 2 3at 914.817 kb on + strand, within Psest_0844at 914.818 kb on - strand, within Psest_0844at 915.046 kb on + strand, within Psest_0844at 915.047 kb on - strand, within Psest_0844at 915.223 kb on - strand, within Psest_0844at 915.288 kb on + strand, within Psest_0844at 915.473 kb on + strandat 915.474 kb on - strandat 915.474 kb on - strandat 915.476 kb on + strandat 915.476 kb on + strandat 915.476 kb on + strandat 915.476 kb on + strandat 915.477 kb on - strandat 915.477 kb on - strandat 915.477 kb on - strandat 915.729 kb on - strand, within Psest_0845at 915.857 kb on - strandat 915.955 kb on + strand, within Psest_0846at 915.955 kb on + strand, within Psest_0846at 915.956 kb on - strand, within Psest_0846at 915.956 kb on - strand, within Psest_0846at 915.967 kb on + strand, within Psest_0846at 916.081 kb on + strand, within Psest_0846at 916.082 kb on - strand, within Psest_0846at 916.082 kb on - strand, within Psest_0846at 916.455 kb on + strandat 916.475 kb on + strandat 916.475 kb on + strandat 916.584 kb on + strandat 916.636 kb on + strandat 916.636 kb on + strandat 916.637 kb on - strandat 916.637 kb on - strandat 916.788 kb on + strand, within Psest_0847at 916.788 kb on + strand, within Psest_0847at 916.788 kb on + strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.789 kb on - strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.856 kb on + strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 916.857 kb on - strand, within Psest_0847at 917.106 kb on + strand, within Psest_0847at 917.107 kb on - strand, within Psest_0847at 917.107 kb on - strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.294 kb on + strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.295 kb on - strand, within Psest_0847at 917.312 kb on - strand, within Psest_0847

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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914,817 + Psest_0844 0.25 +0.2
914,818 - Psest_0844 0.25 -0.8
915,046 + Psest_0844 0.52 -1.4
915,047 - Psest_0844 0.52 -0.7
915,223 - Psest_0844 0.72 +0.2
915,288 + Psest_0844 0.80 -0.9
915,473 + -1.4
915,474 - -0.4
915,474 - -2.0
915,476 + -1.1
915,476 + -1.1
915,476 + +1.2
915,476 + +2.2
915,477 - -1.4
915,477 - +1.2
915,477 - +1.2
915,729 - Psest_0845 0.75 +1.0
915,857 - +1.2
915,955 + Psest_0846 0.27 -1.1
915,955 + Psest_0846 0.27 -0.4
915,956 - Psest_0846 0.27 +1.2
915,956 - Psest_0846 0.27 +1.2
915,967 + Psest_0846 0.29 -1.7
916,081 + Psest_0846 0.50 -0.8
916,082 - Psest_0846 0.50 +2.2
916,082 - Psest_0846 0.50 -2.4
916,455 + -0.3
916,475 + -1.0
916,475 + +1.2
916,584 + -1.6
916,636 + -0.1
916,636 + -1.5
916,637 - -2.3
916,637 - +0.7
916,788 + Psest_0847 0.12 +1.1
916,788 + Psest_0847 0.12 +0.7
916,788 + Psest_0847 0.12 -2.0
916,789 - Psest_0847 0.12 -0.9
916,789 - Psest_0847 0.12 -0.4
916,789 - Psest_0847 0.12 +0.2
916,789 - Psest_0847 0.12 +1.0
916,789 - Psest_0847 0.12 +2.5
916,789 - Psest_0847 0.12 +1.2
916,789 - Psest_0847 0.12 -0.4
916,856 + Psest_0847 0.17 -0.8
916,856 + Psest_0847 0.17 +1.0
916,856 + Psest_0847 0.17 +0.3
916,856 + Psest_0847 0.17 +0.2
916,856 + Psest_0847 0.17 +0.1
916,856 + Psest_0847 0.17 -0.1
916,856 + Psest_0847 0.17 -0.5
916,856 + Psest_0847 0.17 +1.5
916,856 + Psest_0847 0.17 +0.6
916,857 - Psest_0847 0.17 -0.4
916,857 - Psest_0847 0.17 -0.2
916,857 - Psest_0847 0.17 +1.0
916,857 - Psest_0847 0.17 -0.9
916,857 - Psest_0847 0.17 -1.8
916,857 - Psest_0847 0.17 +0.7
916,857 - Psest_0847 0.17 +1.1
916,857 - Psest_0847 0.17 -0.5
916,857 - Psest_0847 0.17 +0.8
917,106 + Psest_0847 0.34 +2.0
917,107 - Psest_0847 0.34 -0.3
917,107 - Psest_0847 0.34 +2.4
917,294 + Psest_0847 0.47 +0.3
917,294 + Psest_0847 0.47 -0.0
917,294 + Psest_0847 0.47 +0.8
917,294 + Psest_0847 0.47 +0.3
917,294 + Psest_0847 0.47 -0.1
917,294 + Psest_0847 0.47 +1.1
917,294 + Psest_0847 0.47 +1.2
917,294 + Psest_0847 0.47 +2.5
917,294 + Psest_0847 0.47 -1.3
917,294 + Psest_0847 0.47 +1.0
917,294 + Psest_0847 0.47 -0.8
917,294 + Psest_0847 0.47 +2.3
917,294 + Psest_0847 0.47 +1.0
917,294 + Psest_0847 0.47 -0.8
917,294 + Psest_0847 0.47 +0.8
917,294 + Psest_0847 0.47 +2.2
917,294 + Psest_0847 0.47 +2.2
917,294 + Psest_0847 0.47 +0.9
917,294 + Psest_0847 0.47 +1.9
917,294 + Psest_0847 0.47 -0.0
917,294 + Psest_0847 0.47 +1.2
917,294 + Psest_0847 0.47 +1.8
917,294 + Psest_0847 0.47 +1.4
917,294 + Psest_0847 0.47 +0.2
917,294 + Psest_0847 0.47 -1.6
917,294 + Psest_0847 0.47 +1.8
917,294 + Psest_0847 0.47 +0.2
917,294 + Psest_0847 0.47 +1.2
917,294 + Psest_0847 0.47 +2.3
917,294 + Psest_0847 0.47 -0.5
917,295 - Psest_0847 0.47 -1.1
917,295 - Psest_0847 0.47 +1.2
917,295 - Psest_0847 0.47 -1.1
917,295 - Psest_0847 0.47 -2.0
917,295 - Psest_0847 0.47 -1.4
917,295 - Psest_0847 0.47 +0.6
917,295 - Psest_0847 0.47 -0.8
917,295 - Psest_0847 0.47 +1.5
917,295 - Psest_0847 0.47 +0.1
917,295 - Psest_0847 0.47 +1.9
917,295 - Psest_0847 0.47 +3.7
917,312 - Psest_0847 0.49 +0.8

Or see this region's nucleotide sequence