Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0810

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0809 and Psest_0810 overlap by 10 nucleotidesPsest_0810 and Psest_0811 are separated by 69 nucleotides Psest_0809: Psest_0809 - Cytochrome c, mono- and diheme variants, at 884,254 to 885,765 _0809 Psest_0810: Psest_0810 - uroporphyrin-III C-methyltransferase, at 885,756 to 886,592 _0810 Psest_0811: Psest_0811 - heme d1 biosynthesis radical SAM protein NirJ, at 886,662 to 887,843 _0811 Position (kb) 885 886 887Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 884.816 kb on + strand, within Psest_0809at 884.816 kb on + strand, within Psest_0809at 884.895 kb on - strand, within Psest_0809at 884.963 kb on + strand, within Psest_0809at 884.964 kb on - strand, within Psest_0809at 884.964 kb on - strand, within Psest_0809at 884.965 kb on + strand, within Psest_0809at 885.068 kb on + strand, within Psest_0809at 885.068 kb on + strand, within Psest_0809at 885.068 kb on + strand, within Psest_0809at 885.068 kb on + strand, within Psest_0809at 885.069 kb on - strand, within Psest_0809at 885.069 kb on - strand, within Psest_0809at 885.069 kb on - strand, within Psest_0809at 885.069 kb on - strand, within Psest_0809at 885.069 kb on - strand, within Psest_0809at 885.069 kb on - strand, within Psest_0809at 885.203 kb on + strand, within Psest_0809at 885.458 kb on + strand, within Psest_0809at 885.459 kb on - strand, within Psest_0809at 885.459 kb on - strand, within Psest_0809at 885.464 kb on + strand, within Psest_0809at 885.465 kb on - strand, within Psest_0809at 885.547 kb on + strand, within Psest_0809at 885.765 kb on + strandat 885.765 kb on + strandat 885.766 kb on - strandat 886.039 kb on + strand, within Psest_0810at 886.039 kb on + strand, within Psest_0810at 886.039 kb on + strand, within Psest_0810at 886.039 kb on + strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.040 kb on - strand, within Psest_0810at 886.171 kb on + strand, within Psest_0810at 886.171 kb on + strand, within Psest_0810at 886.172 kb on - strand, within Psest_0810at 886.388 kb on - strand, within Psest_0810at 886.388 kb on - strand, within Psest_0810at 886.388 kb on - strand, within Psest_0810at 886.447 kb on + strand, within Psest_0810at 886.620 kb on + strandat 886.621 kb on - strandat 886.621 kb on - strandat 886.936 kb on + strand, within Psest_0811at 886.936 kb on + strand, within Psest_0811at 886.936 kb on + strand, within Psest_0811at 886.936 kb on + strand, within Psest_0811at 886.937 kb on - strand, within Psest_0811at 886.937 kb on - strand, within Psest_0811at 886.937 kb on - strand, within Psest_0811at 886.979 kb on + strand, within Psest_0811at 886.997 kb on + strand, within Psest_0811at 887.130 kb on + strand, within Psest_0811at 887.130 kb on + strand, within Psest_0811at 887.130 kb on + strand, within Psest_0811at 887.130 kb on + strand, within Psest_0811at 887.130 kb on + strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.131 kb on - strand, within Psest_0811at 887.227 kb on + strand, within Psest_0811at 887.227 kb on + strand, within Psest_0811at 887.228 kb on - strand, within Psest_0811at 887.228 kb on - strand, within Psest_0811at 887.228 kb on - strand, within Psest_0811at 887.245 kb on + strand, within Psest_0811at 887.245 kb on + strand, within Psest_0811at 887.245 kb on + strand, within Psest_0811at 887.246 kb on - strand, within Psest_0811at 887.302 kb on + strand, within Psest_0811at 887.302 kb on + strand, within Psest_0811at 887.302 kb on + strand, within Psest_0811at 887.303 kb on - strand, within Psest_0811at 887.321 kb on + strand, within Psest_0811at 887.452 kb on + strand, within Psest_0811at 887.453 kb on - strand, within Psest_0811

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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884,816 + Psest_0809 0.37 -0.8
884,816 + Psest_0809 0.37 -2.4
884,895 - Psest_0809 0.42 -0.8
884,963 + Psest_0809 0.47 -3.0
884,964 - Psest_0809 0.47 +0.3
884,964 - Psest_0809 0.47 -0.1
884,965 + Psest_0809 0.47 -0.6
885,068 + Psest_0809 0.54 +1.6
885,068 + Psest_0809 0.54 -1.4
885,068 + Psest_0809 0.54 +0.1
885,068 + Psest_0809 0.54 -0.9
885,069 - Psest_0809 0.54 -2.4
885,069 - Psest_0809 0.54 -0.4
885,069 - Psest_0809 0.54 -1.0
885,069 - Psest_0809 0.54 -0.5
885,069 - Psest_0809 0.54 +0.2
885,069 - Psest_0809 0.54 +0.1
885,203 + Psest_0809 0.63 -1.0
885,458 + Psest_0809 0.80 -1.6
885,459 - Psest_0809 0.80 -1.1
885,459 - Psest_0809 0.80 -0.1
885,464 + Psest_0809 0.80 -1.8
885,465 - Psest_0809 0.80 +0.5
885,547 + Psest_0809 0.86 -1.5
885,765 + -0.4
885,765 + +0.7
885,766 - -0.3
886,039 + Psest_0810 0.34 -2.4
886,039 + Psest_0810 0.34 -1.8
886,039 + Psest_0810 0.34 +1.2
886,039 + Psest_0810 0.34 -1.5
886,040 - Psest_0810 0.34 -2.8
886,040 - Psest_0810 0.34 -2.0
886,040 - Psest_0810 0.34 -2.3
886,040 - Psest_0810 0.34 -1.2
886,040 - Psest_0810 0.34 -0.9
886,040 - Psest_0810 0.34 -3.6
886,040 - Psest_0810 0.34 +0.4
886,040 - Psest_0810 0.34 +2.0
886,171 + Psest_0810 0.50 -0.3
886,171 + Psest_0810 0.50 -2.4
886,172 - Psest_0810 0.50 -1.4
886,388 - Psest_0810 0.76 +0.8
886,388 - Psest_0810 0.76 +0.4
886,388 - Psest_0810 0.76 -0.4
886,447 + Psest_0810 0.83 -0.7
886,620 + -1.6
886,621 - +0.2
886,621 - -1.8
886,936 + Psest_0811 0.23 +1.2
886,936 + Psest_0811 0.23 -2.8
886,936 + Psest_0811 0.23 +0.1
886,936 + Psest_0811 0.23 +1.3
886,937 - Psest_0811 0.23 -3.0
886,937 - Psest_0811 0.23 +0.2
886,937 - Psest_0811 0.23 -0.8
886,979 + Psest_0811 0.27 -3.2
886,997 + Psest_0811 0.28 +0.3
887,130 + Psest_0811 0.40 +1.0
887,130 + Psest_0811 0.40 +0.5
887,130 + Psest_0811 0.40 +0.5
887,130 + Psest_0811 0.40 +0.2
887,130 + Psest_0811 0.40 -1.1
887,131 - Psest_0811 0.40 -0.8
887,131 - Psest_0811 0.40 +0.1
887,131 - Psest_0811 0.40 -0.3
887,131 - Psest_0811 0.40 -2.3
887,131 - Psest_0811 0.40 -2.6
887,131 - Psest_0811 0.40 +1.9
887,131 - Psest_0811 0.40 -1.0
887,227 + Psest_0811 0.48 -0.3
887,227 + Psest_0811 0.48 +0.3
887,228 - Psest_0811 0.48 -2.5
887,228 - Psest_0811 0.48 -1.9
887,228 - Psest_0811 0.48 -2.4
887,245 + Psest_0811 0.49 +0.4
887,245 + Psest_0811 0.49 -3.0
887,245 + Psest_0811 0.49 +4.7
887,246 - Psest_0811 0.49 -0.6
887,302 + Psest_0811 0.54 -4.5
887,302 + Psest_0811 0.54 +0.6
887,302 + Psest_0811 0.54 +1.1
887,303 - Psest_0811 0.54 -0.5
887,321 + Psest_0811 0.56 +0.6
887,452 + Psest_0811 0.67 +1.1
887,453 - Psest_0811 0.67 -2.4

Or see this region's nucleotide sequence