Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0747 and Psest_0748 are separated by 9 nucleotides Psest_0748 and Psest_0749 are separated by 359 nucleotides Psest_0749 and Psest_0750 are separated by 108 nucleotides Psest_0750 and Psest_0751 are separated by 22 nucleotides
Psest_0747: Psest_0747 - Putative GTPases (G3E family), at 818,960 to 820,162
_0747
Psest_0748: Psest_0748 - RNA polymerase-binding protein DksA, at 820,172 to 820,576
_0748
Psest_0749: Psest_0749 - Transcriptional regulator, at 820,936 to 821,646
_0749
Psest_0750: Psest_0750 - hypothetical protein, at 821,755 to 822,129
_0750
Psest_0751: Psest_0751 - Arabinose efflux permease, at 822,152 to 823,333
_0751
Position (kb) 820
821
822 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 820.536 kb on + strand at 820.536 kb on + strand at 820.536 kb on + strand at 820.537 kb on - strand at 820.537 kb on - strand at 820.537 kb on - strand at 820.537 kb on - strand at 820.558 kb on + strand at 820.629 kb on + strand at 820.629 kb on + strand at 820.629 kb on + strand at 820.630 kb on - strand at 820.630 kb on - strand at 820.630 kb on - strand at 820.630 kb on - strand at 820.780 kb on + strand at 820.900 kb on - strand at 820.905 kb on - strand at 821.049 kb on + strand, within Psest_0749 at 821.050 kb on - strand, within Psest_0749 at 821.176 kb on + strand, within Psest_0749 at 821.177 kb on - strand, within Psest_0749 at 821.177 kb on - strand, within Psest_0749 at 821.177 kb on - strand, within Psest_0749 at 821.180 kb on - strand, within Psest_0749 at 821.180 kb on - strand, within Psest_0749 at 821.180 kb on - strand, within Psest_0749 at 821.180 kb on - strand, within Psest_0749 at 821.180 kb on - strand, within Psest_0749 at 821.203 kb on + strand, within Psest_0749 at 821.203 kb on + strand, within Psest_0749 at 821.203 kb on + strand, within Psest_0749 at 821.203 kb on + strand, within Psest_0749 at 821.204 kb on - strand, within Psest_0749 at 821.296 kb on + strand, within Psest_0749 at 821.525 kb on - strand, within Psest_0749 at 821.700 kb on - strand at 821.755 kb on - strand at 821.784 kb on + strand at 821.784 kb on + strand at 821.784 kb on + strand at 821.784 kb on + strand at 821.785 kb on - strand at 821.812 kb on + strand, within Psest_0750 at 821.812 kb on + strand, within Psest_0750 at 821.812 kb on + strand, within Psest_0750 at 821.812 kb on + strand, within Psest_0750 at 821.812 kb on + strand, within Psest_0750 at 821.813 kb on - strand, within Psest_0750 at 821.813 kb on - strand, within Psest_0750 at 821.813 kb on - strand, within Psest_0750 at 821.813 kb on - strand, within Psest_0750 at 821.813 kb on - strand, within Psest_0750 at 821.833 kb on + strand, within Psest_0750 at 821.833 kb on + strand, within Psest_0750 at 822.407 kb on + strand, within Psest_0751
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 820,536 + -0.8 820,536 + -0.5 820,536 + -1.4 820,537 - +0.2 820,537 - +0.2 820,537 - -2.4 820,537 - -0.8 820,558 + -1.2 820,629 + -0.4 820,629 + +1.2 820,629 + +1.2 820,630 - -1.0 820,630 - -1.4 820,630 - -0.6 820,630 - -3.2 820,780 + -2.3 820,900 - -1.4 820,905 - -0.4 821,049 + Psest_0749 0.16 -2.3 821,050 - Psest_0749 0.16 +0.4 821,176 + Psest_0749 0.34 +0.2 821,177 - Psest_0749 0.34 -0.2 821,177 - Psest_0749 0.34 -1.4 821,177 - Psest_0749 0.34 -2.0 821,180 - Psest_0749 0.34 +0.6 821,180 - Psest_0749 0.34 -0.4 821,180 - Psest_0749 0.34 -1.6 821,180 - Psest_0749 0.34 -2.5 821,180 - Psest_0749 0.34 -1.4 821,203 + Psest_0749 0.38 +0.2 821,203 + Psest_0749 0.38 -1.6 821,203 + Psest_0749 0.38 -1.8 821,203 + Psest_0749 0.38 -1.0 821,204 - Psest_0749 0.38 +0.7 821,296 + Psest_0749 0.51 +0.6 821,525 - Psest_0749 0.83 -0.6 821,700 - -0.2 821,755 - -1.0 821,784 + +0.2 821,784 + +1.6 821,784 + -0.2 821,784 + -1.6 821,785 - -1.8 821,812 + Psest_0750 0.15 -0.8 821,812 + Psest_0750 0.15 -0.4 821,812 + Psest_0750 0.15 -1.3 821,812 + Psest_0750 0.15 -1.3 821,812 + Psest_0750 0.15 +0.6 821,813 - Psest_0750 0.15 +0.6 821,813 - Psest_0750 0.15 +1.2 821,813 - Psest_0750 0.15 +0.6 821,813 - Psest_0750 0.15 -2.7 821,813 - Psest_0750 0.15 +0.9 821,833 + Psest_0750 0.21 +0.2 821,833 + Psest_0750 0.21 +0.3 822,407 + Psest_0751 0.22 +0.2
Or see this region's nucleotide sequence