Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0072 and Psest_0073 overlap by 4 nucleotides Psest_0073 and Psest_0074 overlap by 4 nucleotides Psest_0074 and Psest_0075 are separated by 316 nucleotides Psest_0075 and Psest_0076 are separated by 57 nucleotides
Psest_0072: Psest_0072 - hypothetical protein, at 73,209 to 74,363
_0072
Psest_0073: Psest_0073 - ABC-type multidrug transport system, ATPase and permease components, at 74,360 to 76,165
_0073
Psest_0074: Psest_0074 - hypothetical protein, at 76,162 to 76,377
_0074
Psest_0075: Psest_0075 - Uncharacterized conserved protein, at 76,694 to 76,909
_0075
Psest_0076: Psest_0076 - NhaP-type Na+/H+ and K+/H+ antiporters, at 76,967 to 78,331
_0076
Position (kb)
74
75
76
77 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 73.419 kb on + strand, within Psest_0072 at 73.420 kb on - strand, within Psest_0072 at 73.611 kb on + strand, within Psest_0072 at 73.611 kb on + strand, within Psest_0072 at 73.611 kb on + strand, within Psest_0072 at 73.611 kb on + strand, within Psest_0072 at 73.611 kb on + strand, within Psest_0072 at 73.612 kb on - strand, within Psest_0072 at 73.612 kb on - strand, within Psest_0072 at 73.612 kb on - strand, within Psest_0072 at 73.612 kb on - strand, within Psest_0072 at 73.612 kb on - strand, within Psest_0072 at 73.612 kb on - strand, within Psest_0072 at 73.665 kb on + strand, within Psest_0072 at 73.665 kb on + strand, within Psest_0072 at 73.666 kb on - strand, within Psest_0072 at 73.666 kb on - strand, within Psest_0072 at 73.666 kb on - strand, within Psest_0072 at 73.666 kb on - strand, within Psest_0072 at 73.666 kb on - strand, within Psest_0072 at 73.923 kb on + strand, within Psest_0072 at 73.923 kb on + strand, within Psest_0072 at 73.924 kb on - strand, within Psest_0072 at 73.924 kb on - strand, within Psest_0072 at 73.924 kb on - strand, within Psest_0072 at 73.947 kb on + strand, within Psest_0072 at 73.947 kb on + strand, within Psest_0072 at 73.947 kb on + strand, within Psest_0072 at 73.947 kb on + strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.948 kb on - strand, within Psest_0072 at 73.950 kb on + strand, within Psest_0072 at 73.950 kb on + strand, within Psest_0072 at 73.950 kb on + strand, within Psest_0072 at 73.951 kb on - strand, within Psest_0072 at 74.118 kb on + strand, within Psest_0072 at 74.119 kb on - strand, within Psest_0072 at 74.119 kb on - strand, within Psest_0072 at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.441 kb on + strand at 74.442 kb on - strand at 74.442 kb on - strand at 74.442 kb on - strand at 74.442 kb on - strand at 74.442 kb on - strand at 74.535 kb on + strand at 74.535 kb on + strand at 74.536 kb on - strand at 74.810 kb on + strand, within Psest_0073 at 74.810 kb on + strand, within Psest_0073 at 74.810 kb on + strand, within Psest_0073 at 74.810 kb on + strand, within Psest_0073 at 74.811 kb on - strand, within Psest_0073 at 75.135 kb on - strand, within Psest_0073 at 75.135 kb on - strand, within Psest_0073 at 75.135 kb on - strand, within Psest_0073 at 75.487 kb on + strand, within Psest_0073 at 75.689 kb on - strand, within Psest_0073 at 76.172 kb on + strand at 76.173 kb on - strand at 76.173 kb on - strand at 76.258 kb on + strand, within Psest_0074 at 76.338 kb on - strand, within Psest_0074 at 76.338 kb on - strand, within Psest_0074 at 76.442 kb on + strand at 76.443 kb on - strand at 76.521 kb on + strand at 76.521 kb on + strand at 76.522 kb on - strand at 76.522 kb on - strand at 76.522 kb on - strand at 76.522 kb on - strand at 76.522 kb on - strand at 76.522 kb on - strand at 76.712 kb on + strand at 76.712 kb on + strand at 76.712 kb on + strand at 76.712 kb on + strand at 76.712 kb on + strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 76.713 kb on - strand at 77.090 kb on - strand at 77.090 kb on - strand at 77.127 kb on + strand, within Psest_0076 at 77.127 kb on + strand, within Psest_0076 at 77.127 kb on + strand, within Psest_0076 at 77.136 kb on + strand, within Psest_0076 at 77.136 kb on + strand, within Psest_0076 at 77.137 kb on - strand, within Psest_0076 at 77.137 kb on - strand, within Psest_0076 at 77.137 kb on - strand, within Psest_0076 at 77.137 kb on - strand, within Psest_0076 at 77.139 kb on + strand, within Psest_0076 at 77.139 kb on + strand, within Psest_0076 at 77.140 kb on - strand, within Psest_0076 at 77.140 kb on - strand, within Psest_0076 at 77.140 kb on - strand, within Psest_0076
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 73,419 + Psest_0072 0.18 -1.7 73,420 - Psest_0072 0.18 -3.6 73,611 + Psest_0072 0.35 +0.5 73,611 + Psest_0072 0.35 -0.7 73,611 + Psest_0072 0.35 +0.7 73,611 + Psest_0072 0.35 -1.6 73,611 + Psest_0072 0.35 -1.9 73,612 - Psest_0072 0.35 -1.0 73,612 - Psest_0072 0.35 -1.2 73,612 - Psest_0072 0.35 -0.1 73,612 - Psest_0072 0.35 +0.9 73,612 - Psest_0072 0.35 -0.5 73,612 - Psest_0072 0.35 +0.6 73,665 + Psest_0072 0.39 +1.5 73,665 + Psest_0072 0.39 +1.3 73,666 - Psest_0072 0.40 -1.4 73,666 - Psest_0072 0.40 -1.7 73,666 - Psest_0072 0.40 +1.3 73,666 - Psest_0072 0.40 -1.3 73,666 - Psest_0072 0.40 -1.3 73,923 + Psest_0072 0.62 +0.2 73,923 + Psest_0072 0.62 -1.3 73,924 - Psest_0072 0.62 -2.8 73,924 - Psest_0072 0.62 +0.2 73,924 - Psest_0072 0.62 -1.9 73,947 + Psest_0072 0.64 -2.4 73,947 + Psest_0072 0.64 -0.3 73,947 + Psest_0072 0.64 +0.3 73,947 + Psest_0072 0.64 -0.1 73,948 - Psest_0072 0.64 -0.5 73,948 - Psest_0072 0.64 -1.7 73,948 - Psest_0072 0.64 -1.1 73,948 - Psest_0072 0.64 -1.9 73,948 - Psest_0072 0.64 +0.2 73,948 - Psest_0072 0.64 -0.1 73,948 - Psest_0072 0.64 -0.0 73,950 + Psest_0072 0.64 -0.9 73,950 + Psest_0072 0.64 +1.1 73,950 + Psest_0072 0.64 +0.8 73,951 - Psest_0072 0.64 -1.7 74,118 + Psest_0072 0.79 +0.3 74,119 - Psest_0072 0.79 -0.6 74,119 - Psest_0072 0.79 -0.7 74,441 + +0.5 74,441 + -0.8 74,441 + -0.1 74,441 + -1.0 74,441 + -1.6 74,441 + +1.5 74,441 + +0.3 74,441 + +1.0 74,441 + +0.3 74,441 + +3.0 74,441 + +2.1 74,441 + +2.2 74,442 - +0.7 74,442 - -2.8 74,442 - +0.3 74,442 - +0.7 74,442 - -2.5 74,535 + -0.3 74,535 + +1.0 74,536 - +0.6 74,810 + Psest_0073 0.25 -2.0 74,810 + Psest_0073 0.25 -0.9 74,810 + Psest_0073 0.25 -2.8 74,810 + Psest_0073 0.25 -1.5 74,811 - Psest_0073 0.25 -1.2 75,135 - Psest_0073 0.43 -0.3 75,135 - Psest_0073 0.43 -0.3 75,135 - Psest_0073 0.43 -0.9 75,487 + Psest_0073 0.62 +2.0 75,689 - Psest_0073 0.74 -1.7 76,172 + +1.3 76,173 - -1.4 76,173 - -1.9 76,258 + Psest_0074 0.44 +1.6 76,338 - Psest_0074 0.81 -1.0 76,338 - Psest_0074 0.81 -0.7 76,442 + +2.8 76,443 - -0.4 76,521 + -0.3 76,521 + +1.1 76,522 - -1.5 76,522 - -1.3 76,522 - -0.3 76,522 - +2.0 76,522 - +0.9 76,522 - +0.2 76,712 + +0.3 76,712 + +1.3 76,712 + -1.0 76,712 + -0.7 76,712 + -0.0 76,713 - +0.3 76,713 - -0.3 76,713 - -0.2 76,713 - -1.4 76,713 - +0.3 76,713 - +0.2 76,713 - -1.3 76,713 - +2.3 76,713 - -2.4 76,713 - +2.5 76,713 - -0.1 77,090 - -2.2 77,090 - +1.3 77,127 + Psest_0076 0.12 -2.2 77,127 + Psest_0076 0.12 +1.3 77,127 + Psest_0076 0.12 +0.3 77,136 + Psest_0076 0.12 +0.3 77,136 + Psest_0076 0.12 +0.3 77,137 - Psest_0076 0.12 +0.7 77,137 - Psest_0076 0.12 -0.5 77,137 - Psest_0076 0.12 +0.9 77,137 - Psest_0076 0.12 +2.4 77,139 + Psest_0076 0.13 -1.0 77,139 + Psest_0076 0.13 -2.0 77,140 - Psest_0076 0.13 -1.0 77,140 - Psest_0076 0.13 -1.0 77,140 - Psest_0076 0.13 -1.5
Or see this region's nucleotide sequence