Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0723 and Psest_0724 are separated by 156 nucleotides Psest_0724 and Psest_0725 are separated by 104 nucleotides Psest_0725 and Psest_0726 are separated by 16 nucleotides
Psest_0723: Psest_0723 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 792,717 to 793,706
_0723
Psest_0724: Psest_0724 - Deoxyinosine 3'endonuclease (endonuclease V), at 793,863 to 794,591
_0724
Psest_0725: Psest_0725 - Negative regulator of beta-lactamase expression, at 794,696 to 795,247
_0725
Psest_0726: Psest_0726 - Membrane protein required for beta-lactamase induction, at 795,264 to 796,100
_0726
Position (kb) 793
794
795 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4
5 at 792.891 kb on + strand, within Psest_0723 at 792.891 kb on + strand, within Psest_0723 at 792.891 kb on + strand, within Psest_0723 at 792.892 kb on - strand, within Psest_0723 at 792.892 kb on - strand, within Psest_0723 at 792.892 kb on - strand, within Psest_0723 at 792.892 kb on - strand, within Psest_0723 at 792.892 kb on - strand, within Psest_0723 at 792.892 kb on - strand, within Psest_0723 at 792.896 kb on - strand, within Psest_0723 at 792.896 kb on - strand, within Psest_0723 at 792.896 kb on - strand, within Psest_0723 at 793.088 kb on - strand, within Psest_0723 at 793.165 kb on + strand, within Psest_0723 at 793.165 kb on + strand, within Psest_0723 at 793.165 kb on + strand, within Psest_0723 at 793.165 kb on + strand, within Psest_0723 at 793.166 kb on - strand, within Psest_0723 at 793.166 kb on - strand, within Psest_0723 at 793.166 kb on - strand, within Psest_0723 at 793.166 kb on - strand, within Psest_0723 at 793.166 kb on - strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.171 kb on + strand, within Psest_0723 at 793.172 kb on - strand, within Psest_0723 at 793.172 kb on - strand, within Psest_0723 at 793.172 kb on - strand, within Psest_0723 at 793.172 kb on - strand, within Psest_0723 at 793.172 kb on - strand, within Psest_0723 at 793.172 kb on - strand, within Psest_0723 at 793.208 kb on - strand, within Psest_0723 at 793.671 kb on + strand at 793.672 kb on - strand at 793.672 kb on - strand at 793.714 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.718 kb on + strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 793.719 kb on - strand at 794.008 kb on - strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.121 kb on + strand, within Psest_0724 at 794.122 kb on - strand, within Psest_0724 at 794.122 kb on - strand, within Psest_0724 at 794.266 kb on + strand, within Psest_0724 at 794.395 kb on + strand, within Psest_0724 at 794.605 kb on + strand at 794.781 kb on + strand, within Psest_0725 at 794.781 kb on + strand, within Psest_0725 at 794.793 kb on + strand, within Psest_0725 at 794.794 kb on - strand, within Psest_0725 at 794.794 kb on - strand, within Psest_0725 at 795.055 kb on + strand, within Psest_0725 at 795.238 kb on + strand at 795.239 kb on - strand at 795.239 kb on - strand at 795.415 kb on + strand, within Psest_0726 at 795.519 kb on + strand, within Psest_0726 at 795.519 kb on + strand, within Psest_0726 at 795.520 kb on - strand, within Psest_0726 at 795.520 kb on - strand, within Psest_0726
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 792,891 + Psest_0723 0.18 +1.2 792,891 + Psest_0723 0.18 +1.2 792,891 + Psest_0723 0.18 +1.7 792,892 - Psest_0723 0.18 +0.9 792,892 - Psest_0723 0.18 -2.9 792,892 - Psest_0723 0.18 -1.3 792,892 - Psest_0723 0.18 -1.2 792,892 - Psest_0723 0.18 +0.1 792,892 - Psest_0723 0.18 -2.9 792,896 - Psest_0723 0.18 -0.2 792,896 - Psest_0723 0.18 +1.2 792,896 - Psest_0723 0.18 -0.4 793,088 - Psest_0723 0.37 -3.2 793,165 + Psest_0723 0.45 +0.7 793,165 + Psest_0723 0.45 -0.8 793,165 + Psest_0723 0.45 -0.1 793,165 + Psest_0723 0.45 +2.7 793,166 - Psest_0723 0.45 +0.9 793,166 - Psest_0723 0.45 -2.9 793,166 - Psest_0723 0.45 +0.3 793,166 - Psest_0723 0.45 -2.1 793,166 - Psest_0723 0.45 +1.4 793,171 + Psest_0723 0.46 -2.1 793,171 + Psest_0723 0.46 +0.2 793,171 + Psest_0723 0.46 +2.8 793,171 + Psest_0723 0.46 -3.2 793,171 + Psest_0723 0.46 -0.2 793,171 + Psest_0723 0.46 +1.4 793,171 + Psest_0723 0.46 +1.2 793,171 + Psest_0723 0.46 +1.5 793,171 + Psest_0723 0.46 +0.5 793,171 + Psest_0723 0.46 -2.3 793,171 + Psest_0723 0.46 +0.4 793,171 + Psest_0723 0.46 +0.4 793,171 + Psest_0723 0.46 -0.0 793,171 + Psest_0723 0.46 +0.1 793,171 + Psest_0723 0.46 -0.4 793,171 + Psest_0723 0.46 +0.9 793,171 + Psest_0723 0.46 +1.8 793,171 + Psest_0723 0.46 -2.0 793,172 - Psest_0723 0.46 +0.3 793,172 - Psest_0723 0.46 -0.6 793,172 - Psest_0723 0.46 -0.5 793,172 - Psest_0723 0.46 -1.4 793,172 - Psest_0723 0.46 -1.4 793,172 - Psest_0723 0.46 +3.4 793,208 - Psest_0723 0.50 +0.6 793,671 + +1.7 793,672 - -5.3 793,672 - -4.0 793,714 + -0.4 793,718 + -3.7 793,718 + +1.2 793,718 + -1.7 793,718 + +0.4 793,718 + -2.6 793,718 + +1.7 793,718 + -0.3 793,718 + -3.0 793,718 + -1.1 793,718 + +1.5 793,719 - -2.5 793,719 - +1.4 793,719 - -1.8 793,719 - +2.3 793,719 - -4.2 793,719 - -0.8 793,719 - -1.1 793,719 - -2.5 794,008 - Psest_0724 0.20 +2.4 794,121 + Psest_0724 0.35 +0.2 794,121 + Psest_0724 0.35 +1.3 794,121 + Psest_0724 0.35 +1.8 794,121 + Psest_0724 0.35 -0.8 794,122 - Psest_0724 0.36 +1.4 794,122 - Psest_0724 0.36 +1.2 794,266 + Psest_0724 0.55 -0.9 794,395 + Psest_0724 0.73 +1.5 794,605 + -1.6 794,781 + Psest_0725 0.15 +4.8 794,781 + Psest_0725 0.15 -0.3 794,793 + Psest_0725 0.18 -0.4 794,794 - Psest_0725 0.18 -0.2 794,794 - Psest_0725 0.18 +2.9 795,055 + Psest_0725 0.65 -1.4 795,238 + -0.3 795,239 - +2.3 795,239 - +2.8 795,415 + Psest_0726 0.18 +2.7 795,519 + Psest_0726 0.30 +1.5 795,519 + Psest_0726 0.30 -2.3 795,520 - Psest_0726 0.31 -3.4 795,520 - Psest_0726 0.31 +0.3
Or see this region's nucleotide sequence