Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0499 and Psest_0500 are separated by 28 nucleotides Psest_0500 and Psest_0501 are separated by 186 nucleotides Psest_0501 and Psest_0502 are separated by 19 nucleotides
Psest_0499: Psest_0499 - hypothetical protein, at 554,082 to 554,888
_0499
Psest_0500: Psest_0500 - Oligoribonuclease (3'->5' exoribonuclease), at 554,917 to 555,459
_0500
Psest_0501: Psest_0501 - Predicted nucleoside-diphosphate-sugar epimerases, at 555,646 to 556,287
_0501
Psest_0502: Psest_0502 - Uncharacterized protein conserved in bacteria, at 556,307 to 557,980
_0502
Position (kb) 555
556
557 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 554.668 kb on - strand, within Psest_0499 at 554.668 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 555.290 kb on - strand, within Psest_0500 at 555.290 kb on - strand, within Psest_0500 at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.541 kb on + strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.542 kb on - strand at 555.560 kb on + strand at 555.560 kb on + strand at 555.560 kb on + strand at 555.560 kb on + strand at 555.561 kb on - strand at 555.561 kb on - strand at 555.561 kb on - strand at 555.758 kb on + strand, within Psest_0501 at 555.758 kb on + strand, within Psest_0501 at 555.759 kb on - strand, within Psest_0501 at 555.914 kb on + strand, within Psest_0501 at 555.914 kb on + strand, within Psest_0501 at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.283 kb on + strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.284 kb on - strand at 556.393 kb on - strand at 556.393 kb on - strand at 556.500 kb on + strand, within Psest_0502 at 556.500 kb on + strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.501 kb on - strand, within Psest_0502 at 556.608 kb on + strand, within Psest_0502 at 556.625 kb on + strand, within Psest_0502 at 556.626 kb on - strand, within Psest_0502 at 556.626 kb on - strand, within Psest_0502 at 556.626 kb on - strand, within Psest_0502 at 556.716 kb on - strand, within Psest_0502 at 556.752 kb on + strand, within Psest_0502 at 556.753 kb on - strand, within Psest_0502 at 556.905 kb on - strand, within Psest_0502 at 556.917 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.972 kb on + strand, within Psest_0502 at 556.973 kb on - strand, within Psest_0502 at 556.973 kb on - strand, within Psest_0502 at 556.973 kb on - strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.974 kb on + strand, within Psest_0502 at 556.975 kb on - strand, within Psest_0502 at 556.975 kb on - strand, within Psest_0502 at 556.975 kb on - strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.193 kb on + strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.194 kb on - strand, within Psest_0502 at 557.235 kb on + strand, within Psest_0502 at 557.262 kb on + strand, within Psest_0502 at 557.263 kb on - strand, within Psest_0502
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 554,668 - Psest_0499 0.73 -3.0 554,668 - Psest_0499 0.73 +0.5 554,680 - Psest_0499 0.74 -2.7 554,680 - Psest_0499 0.74 -0.8 554,680 - Psest_0499 0.74 -2.6 555,290 - Psest_0500 0.69 +0.2 555,290 - Psest_0500 0.69 -0.4 555,541 + +1.8 555,541 + -0.1 555,541 + -1.6 555,541 + +0.8 555,541 + -1.3 555,541 + +0.4 555,541 + +0.4 555,541 + +0.6 555,541 + -2.5 555,542 - -1.0 555,542 - -0.4 555,542 - -0.9 555,542 - +0.3 555,542 - -1.3 555,542 - +0.2 555,542 - -0.6 555,560 + -2.6 555,560 + +2.2 555,560 + -1.6 555,560 + -2.3 555,561 - -0.3 555,561 - +0.0 555,561 - -2.2 555,758 + Psest_0501 0.17 -2.0 555,758 + Psest_0501 0.17 -3.5 555,759 - Psest_0501 0.18 -0.6 555,914 + Psest_0501 0.42 +0.7 555,914 + Psest_0501 0.42 -1.1 556,283 + +0.3 556,283 + -0.4 556,283 + +1.2 556,283 + -0.8 556,283 + +0.8 556,283 + -2.5 556,283 + +0.7 556,283 + +1.3 556,283 + +1.5 556,284 - -0.4 556,284 - -1.3 556,284 - +1.2 556,284 - -1.1 556,284 - -2.7 556,284 - +0.9 556,284 - +0.1 556,284 - +0.2 556,284 - -1.4 556,284 - +0.4 556,284 - +3.8 556,284 - +0.8 556,284 - -0.6 556,284 - +0.8 556,284 - -3.5 556,284 - -0.2 556,284 - +0.7 556,284 - -2.5 556,284 - -1.6 556,284 - -1.4 556,284 - +1.0 556,284 - -0.9 556,284 - +0.9 556,284 - +2.5 556,284 - +0.0 556,284 - +1.5 556,284 - +1.6 556,393 - +0.5 556,393 - +0.5 556,500 + Psest_0502 0.12 -1.6 556,500 + Psest_0502 0.12 -0.1 556,501 - Psest_0502 0.12 -2.3 556,501 - Psest_0502 0.12 +0.5 556,501 - Psest_0502 0.12 +1.4 556,501 - Psest_0502 0.12 -2.6 556,608 + Psest_0502 0.18 -0.8 556,625 + Psest_0502 0.19 +1.7 556,626 - Psest_0502 0.19 +0.2 556,626 - Psest_0502 0.19 -1.8 556,626 - Psest_0502 0.19 -0.6 556,716 - Psest_0502 0.24 +0.2 556,752 + Psest_0502 0.27 +0.6 556,753 - Psest_0502 0.27 -2.8 556,905 - Psest_0502 0.36 +1.2 556,917 + Psest_0502 0.36 -0.0 556,972 + Psest_0502 0.40 -4.0 556,972 + Psest_0502 0.40 -2.2 556,972 + Psest_0502 0.40 -0.6 556,972 + Psest_0502 0.40 -1.1 556,972 + Psest_0502 0.40 -1.6 556,972 + Psest_0502 0.40 -1.5 556,972 + Psest_0502 0.40 +0.7 556,973 - Psest_0502 0.40 -0.7 556,973 - Psest_0502 0.40 -2.7 556,973 - Psest_0502 0.40 -2.7 556,974 + Psest_0502 0.40 -1.7 556,974 + Psest_0502 0.40 -1.8 556,974 + Psest_0502 0.40 +1.7 556,974 + Psest_0502 0.40 -1.4 556,974 + Psest_0502 0.40 -0.4 556,975 - Psest_0502 0.40 -0.3 556,975 - Psest_0502 0.40 -3.2 556,975 - Psest_0502 0.40 +0.5 557,193 + Psest_0502 0.53 -0.3 557,193 + Psest_0502 0.53 -1.2 557,193 + Psest_0502 0.53 -0.2 557,193 + Psest_0502 0.53 +0.9 557,193 + Psest_0502 0.53 +0.4 557,193 + Psest_0502 0.53 +0.8 557,193 + Psest_0502 0.53 +0.9 557,194 - Psest_0502 0.53 -2.5 557,194 - Psest_0502 0.53 -0.2 557,194 - Psest_0502 0.53 -1.7 557,194 - Psest_0502 0.53 -2.2 557,194 - Psest_0502 0.53 +1.0 557,194 - Psest_0502 0.53 +0.3 557,235 + Psest_0502 0.55 -0.4 557,262 + Psest_0502 0.57 +0.5 557,263 - Psest_0502 0.57 -0.4
Or see this region's nucleotide sequence