Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0496 and Psest_0497 are separated by 9 nucleotides Psest_0497 and Psest_0498 are separated by 9 nucleotides Psest_0498 and Psest_0499 are separated by 123 nucleotides Psest_0499 and Psest_0500 are separated by 28 nucleotides
Psest_0496: Psest_0496 - flagellar motor stator protein MotA, at 551,076 to 551,927
_0496
Psest_0497: Psest_0497 - Flagellar motor protein, at 551,937 to 552,917
_0497
Psest_0498: Psest_0498 - ribosome small subunit-dependent GTPase A, at 552,927 to 553,958
_0498
Psest_0499: Psest_0499 - hypothetical protein, at 554,082 to 554,888
_0499
Psest_0500: Psest_0500 - Oligoribonuclease (3'->5' exoribonuclease), at 554,917 to 555,459
_0500
Position (kb) 552
553
554 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 552.101 kb on + strand, within Psest_0497 at 552.101 kb on + strand, within Psest_0497 at 552.102 kb on - strand, within Psest_0497 at 552.112 kb on - strand, within Psest_0497 at 552.459 kb on + strand, within Psest_0497 at 552.459 kb on + strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.460 kb on - strand, within Psest_0497 at 552.491 kb on + strand, within Psest_0497 at 552.492 kb on - strand, within Psest_0497 at 552.722 kb on + strand, within Psest_0497 at 552.826 kb on + strand at 552.838 kb on + strand at 552.931 kb on + strand at 552.932 kb on - strand at 552.932 kb on - strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.977 kb on + strand at 552.978 kb on - strand at 552.978 kb on - strand at 552.978 kb on - strand at 553.209 kb on - strand, within Psest_0498 at 553.396 kb on + strand, within Psest_0498 at 553.411 kb on + strand, within Psest_0498 at 553.963 kb on + strand at 553.963 kb on + strand at 553.964 kb on - strand at 553.964 kb on - strand at 553.993 kb on + strand at 553.993 kb on + strand at 553.993 kb on + strand at 553.993 kb on + strand at 553.993 kb on + strand at 553.994 kb on - strand at 554.046 kb on + strand at 554.046 kb on + strand at 554.047 kb on - strand at 554.047 kb on - strand at 554.047 kb on - strand at 554.099 kb on + strand at 554.137 kb on + strand at 554.415 kb on + strand, within Psest_0499 at 554.415 kb on + strand, within Psest_0499 at 554.415 kb on + strand, within Psest_0499 at 554.416 kb on - strand, within Psest_0499 at 554.416 kb on - strand, within Psest_0499 at 554.416 kb on - strand, within Psest_0499 at 554.668 kb on - strand, within Psest_0499 at 554.668 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499 at 554.680 kb on - strand, within Psest_0499
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 552,101 + Psest_0497 0.17 +0.7 552,101 + Psest_0497 0.17 +1.9 552,102 - Psest_0497 0.17 +1.7 552,112 - Psest_0497 0.18 -3.6 552,459 + Psest_0497 0.53 +0.7 552,459 + Psest_0497 0.53 -0.4 552,460 - Psest_0497 0.53 +0.5 552,460 - Psest_0497 0.53 +1.1 552,460 - Psest_0497 0.53 -3.3 552,460 - Psest_0497 0.53 +0.3 552,491 + Psest_0497 0.56 -1.1 552,492 - Psest_0497 0.57 +1.0 552,722 + Psest_0497 0.80 +1.1 552,826 + -2.3 552,838 + -3.6 552,931 + +0.8 552,932 - -0.2 552,932 - -3.4 552,977 + -4.5 552,977 + -3.0 552,977 + -3.2 552,977 + -0.6 552,977 + -2.0 552,978 - -3.4 552,978 - -2.3 552,978 - -2.2 553,209 - Psest_0498 0.27 -2.5 553,396 + Psest_0498 0.45 -0.8 553,411 + Psest_0498 0.47 -4.4 553,963 + -3.4 553,963 + -1.8 553,964 - +1.7 553,964 - -0.8 553,993 + -0.8 553,993 + +0.4 553,993 + -1.5 553,993 + -2.3 553,993 + -1.8 553,994 - -1.8 554,046 + -0.6 554,046 + +0.2 554,047 - +0.4 554,047 - -0.6 554,047 - -1.0 554,099 + -2.3 554,137 + +0.6 554,415 + Psest_0499 0.41 +0.1 554,415 + Psest_0499 0.41 +0.5 554,415 + Psest_0499 0.41 +0.6 554,416 - Psest_0499 0.41 +0.1 554,416 - Psest_0499 0.41 -2.8 554,416 - Psest_0499 0.41 -1.8 554,668 - Psest_0499 0.73 -3.0 554,668 - Psest_0499 0.73 +0.5 554,680 - Psest_0499 0.74 -2.7 554,680 - Psest_0499 0.74 -0.8 554,680 - Psest_0499 0.74 -2.6
Or see this region's nucleotide sequence