Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0493 and Psest_0494 are separated by 11 nucleotides Psest_0494 and Psest_0495 are separated by 47 nucleotides
Psest_0493: Psest_0493 - phosphatidylserine decarboxylase precursor, at 547,623 to 548,522
_0493
Psest_0494: Psest_0494 - Rhodanese-related sulfurtransferase, at 548,534 to 549,349
_0494
Psest_0495: Psest_0495 - HDOD domain., at 549,397 to 550,911
_0495
Position (kb) 548
549
550 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 547.563 kb on + strand at 548.589 kb on + strand at 548.632 kb on - strand, within Psest_0494 at 548.632 kb on - strand, within Psest_0494 at 548.632 kb on - strand, within Psest_0494 at 548.632 kb on - strand, within Psest_0494 at 548.632 kb on - strand, within Psest_0494 at 548.736 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 548.893 kb on - strand, within Psest_0494 at 549.091 kb on - strand, within Psest_0494 at 549.091 kb on - strand, within Psest_0494 at 549.091 kb on - strand, within Psest_0494 at 549.100 kb on - strand, within Psest_0494 at 549.100 kb on - strand, within Psest_0494 at 549.100 kb on - strand, within Psest_0494 at 549.370 kb on - strand at 549.370 kb on - strand at 549.370 kb on - strand at 549.370 kb on - strand at 549.668 kb on - strand, within Psest_0495 at 549.668 kb on - strand, within Psest_0495 at 549.668 kb on - strand, within Psest_0495 at 549.668 kb on - strand, within Psest_0495 at 549.668 kb on - strand, within Psest_0495 at 549.832 kb on + strand, within Psest_0495 at 549.832 kb on + strand, within Psest_0495 at 549.832 kb on + strand, within Psest_0495 at 549.832 kb on + strand, within Psest_0495 at 549.832 kb on + strand, within Psest_0495 at 550.064 kb on + strand, within Psest_0495 at 550.064 kb on + strand, within Psest_0495 at 550.064 kb on + strand, within Psest_0495 at 550.064 kb on + strand, within Psest_0495 at 550.065 kb on - strand, within Psest_0495 at 550.065 kb on - strand, within Psest_0495 at 550.065 kb on - strand, within Psest_0495
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 547,563 + +1.0 548,589 + -1.4 548,632 - Psest_0494 0.12 -0.8 548,632 - Psest_0494 0.12 +1.0 548,632 - Psest_0494 0.12 -4.0 548,632 - Psest_0494 0.12 -2.3 548,632 - Psest_0494 0.12 -0.2 548,736 - Psest_0494 0.25 -2.6 548,893 - Psest_0494 0.44 -0.5 548,893 - Psest_0494 0.44 -3.2 548,893 - Psest_0494 0.44 -1.4 548,893 - Psest_0494 0.44 +0.0 548,893 - Psest_0494 0.44 +0.5 548,893 - Psest_0494 0.44 -2.9 548,893 - Psest_0494 0.44 -4.0 548,893 - Psest_0494 0.44 -3.3 548,893 - Psest_0494 0.44 -0.6 548,893 - Psest_0494 0.44 -4.0 548,893 - Psest_0494 0.44 -3.8 548,893 - Psest_0494 0.44 +1.5 548,893 - Psest_0494 0.44 +1.5 548,893 - Psest_0494 0.44 -1.6 548,893 - Psest_0494 0.44 -2.0 548,893 - Psest_0494 0.44 -1.6 548,893 - Psest_0494 0.44 +0.5 549,091 - Psest_0494 0.68 +0.4 549,091 - Psest_0494 0.68 -1.7 549,091 - Psest_0494 0.68 -2.2 549,100 - Psest_0494 0.69 -4.5 549,100 - Psest_0494 0.69 -2.3 549,100 - Psest_0494 0.69 -1.8 549,370 - +1.0 549,370 - +0.6 549,370 - -3.3 549,370 - -2.8 549,668 - Psest_0495 0.18 +0.4 549,668 - Psest_0495 0.18 -1.7 549,668 - Psest_0495 0.18 +0.7 549,668 - Psest_0495 0.18 -3.2 549,668 - Psest_0495 0.18 -1.4 549,832 + Psest_0495 0.29 -1.8 549,832 + Psest_0495 0.29 -0.8 549,832 + Psest_0495 0.29 +0.6 549,832 + Psest_0495 0.29 +0.6 549,832 + Psest_0495 0.29 -1.8 550,064 + Psest_0495 0.44 +0.3 550,064 + Psest_0495 0.44 -3.8 550,064 + Psest_0495 0.44 +1.2 550,064 + Psest_0495 0.44 -0.5 550,065 - Psest_0495 0.44 -1.0 550,065 - Psest_0495 0.44 +2.6 550,065 - Psest_0495 0.44 -0.0
Or see this region's nucleotide sequence