Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0473 and Psest_0474 overlap by 8 nucleotides Psest_0474 and Psest_0475 are separated by 112 nucleotides
Psest_0473: Psest_0473 - Glycerol-3-phosphate dehydrogenase, at 521,763 to 522,938
_0473
Psest_0474: Psest_0474 - Membrane transporters of cations and cationic drugs, at 522,931 to 523,329
_0474
Psest_0475: Psest_0475 - 3-deoxy-D-manno-octulosonic-acid transferase, at 523,442 to 524,710
_0475
Position (kb) 522
523
524 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 522.952 kb on - strand at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.313 kb on + strand at 523.313 kb on + strand at 523.314 kb on - strand at 523.345 kb on - strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 522,952 - -1.6 522,973 - Psest_0474 0.11 -2.4 522,973 - Psest_0474 0.11 -1.3 522,973 - Psest_0474 0.11 -0.2 522,973 - Psest_0474 0.11 +2.3 522,973 - Psest_0474 0.11 -1.5 522,974 + Psest_0474 0.11 +1.3 522,974 + Psest_0474 0.11 -2.5 522,974 + Psest_0474 0.11 +0.9 522,974 + Psest_0474 0.11 -1.1 522,974 + Psest_0474 0.11 -2.4 522,975 - Psest_0474 0.11 -0.0 522,975 - Psest_0474 0.11 -0.3 522,975 - Psest_0474 0.11 -2.9 522,975 - Psest_0474 0.11 -2.1 522,975 - Psest_0474 0.11 -0.4 522,975 - Psest_0474 0.11 -0.5 522,975 - Psest_0474 0.11 -1.8 522,975 - Psest_0474 0.11 -2.3 523,151 + Psest_0474 0.55 +1.1 523,151 + Psest_0474 0.55 +1.0 523,313 + -0.2 523,313 + -1.1 523,314 - +2.9 523,345 - -1.2 523,457 + +0.7 523,457 + +0.5 523,457 + -2.3 523,457 + +0.0 523,457 + +0.7
Or see this region's nucleotide sequence