Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0470 and Psest_0471 are separated by 34 nucleotides Psest_0471 and Psest_0472 are separated by 85 nucleotides Psest_0472 and Psest_0473 overlap by 4 nucleotides
Psest_0470: Psest_0470 - rfaE bifunctional protein, domain I/rfaE bifunctional protein, domain II, at 518,528 to 519,949
_0470
Psest_0471: Psest_0471 - hypothetical protein, at 519,984 to 520,871
_0471
Psest_0472: Psest_0472 - Predicted oxidoreductases (related to aryl-alcohol dehydrogenases), at 520,957 to 521,766
_0472
Psest_0473: Psest_0473 - Glycerol-3-phosphate dehydrogenase, at 521,763 to 522,938
_0473
Position (kb) 519
520
521 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 520.100 kb on + strand, within Psest_0471 at 520.128 kb on + strand, within Psest_0471 at 520.129 kb on - strand, within Psest_0471 at 520.129 kb on - strand, within Psest_0471 at 520.129 kb on - strand, within Psest_0471 at 520.178 kb on - strand, within Psest_0471 at 520.178 kb on - strand, within Psest_0471 at 520.178 kb on - strand, within Psest_0471 at 520.178 kb on - strand, within Psest_0471 at 520.284 kb on - strand, within Psest_0471 at 520.294 kb on + strand, within Psest_0471 at 520.295 kb on - strand, within Psest_0471 at 520.330 kb on + strand, within Psest_0471 at 520.330 kb on + strand, within Psest_0471 at 520.331 kb on - strand, within Psest_0471 at 520.331 kb on - strand, within Psest_0471 at 520.572 kb on - strand, within Psest_0471 at 520.573 kb on + strand, within Psest_0471 at 520.573 kb on + strand, within Psest_0471 at 520.574 kb on - strand, within Psest_0471 at 520.574 kb on - strand, within Psest_0471 at 520.786 kb on + strand at 520.787 kb on - strand at 520.787 kb on - strand at 520.816 kb on + strand at 520.816 kb on + strand at 520.817 kb on - strand at 520.817 kb on - strand at 520.817 kb on - strand at 520.854 kb on + strand at 520.855 kb on - strand at 520.866 kb on - strand at 520.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 520,100 + Psest_0471 0.13 -3.5 520,128 + Psest_0471 0.16 -1.4 520,129 - Psest_0471 0.16 -1.2 520,129 - Psest_0471 0.16 -4.2 520,129 - Psest_0471 0.16 -1.6 520,178 - Psest_0471 0.22 -2.0 520,178 - Psest_0471 0.22 -2.0 520,178 - Psest_0471 0.22 -1.2 520,178 - Psest_0471 0.22 +0.8 520,284 - Psest_0471 0.34 -2.9 520,294 + Psest_0471 0.35 -1.1 520,295 - Psest_0471 0.35 +1.2 520,330 + Psest_0471 0.39 -0.2 520,330 + Psest_0471 0.39 -0.4 520,331 - Psest_0471 0.39 -0.5 520,331 - Psest_0471 0.39 -0.5 520,572 - Psest_0471 0.66 -0.3 520,573 + Psest_0471 0.66 -2.5 520,573 + Psest_0471 0.66 -0.9 520,574 - Psest_0471 0.66 -1.1 520,574 - Psest_0471 0.66 +1.0 520,786 + -0.8 520,787 - -0.3 520,787 - -2.4 520,816 + -2.7 520,816 + -0.4 520,817 - -0.7 520,817 - +0.0 520,817 - -0.1 520,854 + -0.3 520,855 - -1.8 520,866 - -1.1 520,866 - -1.4
Or see this region's nucleotide sequence