Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4202 and Psest_4203 are separated by 521 nucleotides Psest_4203 and Psest_4204 are separated by 13 nucleotides Psest_4204 and Psest_4205 are separated by 3 nucleotides
Psest_4202: Psest_4202 - general secretory pathway protein E, at 4,379,434 to 4,380,948
_4202
Psest_4203: Psest_4203 - NAD/NADP transhydrogenase alpha subunit, at 4,381,470 to 4,382,591
_4203
Psest_4204: Psest_4204 - hypothetical protein, at 4,382,605 to 4,382,922
_4204
Psest_4205: Psest_4205 - NAD/NADP transhydrogenase beta subunit, at 4,382,926 to 4,384,362
_4205
Position (kb)
4381
4382
4383 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4380.705 kb on - strand, within Psest_4202 at 4380.931 kb on + strand at 4380.931 kb on + strand at 4380.931 kb on + strand at 4380.932 kb on - strand at 4380.932 kb on - strand at 4380.932 kb on - strand at 4380.932 kb on - strand at 4380.932 kb on - strand at 4381.024 kb on + strand at 4381.024 kb on + strand at 4381.024 kb on + strand at 4381.024 kb on + strand at 4381.034 kb on - strand at 4381.273 kb on - strand at 4381.273 kb on - strand at 4381.617 kb on + strand, within Psest_4203 at 4381.618 kb on - strand, within Psest_4203 at 4381.618 kb on - strand, within Psest_4203 at 4381.826 kb on + strand, within Psest_4203 at 4381.827 kb on - strand, within Psest_4203 at 4381.827 kb on - strand, within Psest_4203 at 4381.827 kb on - strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.852 kb on + strand, within Psest_4203 at 4381.853 kb on - strand, within Psest_4203 at 4381.853 kb on - strand, within Psest_4203 at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.629 kb on + strand at 4382.630 kb on - strand at 4382.630 kb on - strand at 4382.630 kb on - strand at 4382.630 kb on - strand at 4382.630 kb on - strand at 4382.659 kb on + strand, within Psest_4204 at 4382.659 kb on + strand, within Psest_4204 at 4382.659 kb on + strand, within Psest_4204 at 4382.659 kb on + strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.660 kb on - strand, within Psest_4204 at 4382.722 kb on - strand, within Psest_4204 at 4382.920 kb on + strand at 4382.953 kb on + strand at 4382.953 kb on + strand at 4382.954 kb on - strand at 4382.954 kb on - strand at 4383.059 kb on + strand at 4383.060 kb on - strand at 4383.122 kb on - strand, within Psest_4205 at 4383.242 kb on + strand, within Psest_4205 at 4383.409 kb on + strand, within Psest_4205 at 4383.409 kb on + strand, within Psest_4205 at 4383.410 kb on - strand, within Psest_4205 at 4383.410 kb on - strand, within Psest_4205 at 4383.410 kb on - strand, within Psest_4205 at 4383.508 kb on + strand, within Psest_4205 at 4383.508 kb on + strand, within Psest_4205
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 4,380,705 - Psest_4202 0.84 -1.2 4,380,931 + +1.4 4,380,931 + -0.2 4,380,931 + -1.0 4,380,932 - +1.1 4,380,932 - -0.8 4,380,932 - +1.4 4,380,932 - -0.6 4,380,932 - -0.2 4,381,024 + +2.1 4,381,024 + -0.4 4,381,024 + +1.2 4,381,024 + -0.2 4,381,034 - +3.4 4,381,273 - -1.3 4,381,273 - -0.6 4,381,617 + Psest_4203 0.13 -0.5 4,381,618 - Psest_4203 0.13 +0.7 4,381,618 - Psest_4203 0.13 +0.1 4,381,826 + Psest_4203 0.32 -0.2 4,381,827 - Psest_4203 0.32 -2.1 4,381,827 - Psest_4203 0.32 -1.0 4,381,827 - Psest_4203 0.32 -1.2 4,381,852 + Psest_4203 0.34 +1.7 4,381,852 + Psest_4203 0.34 -1.2 4,381,852 + Psest_4203 0.34 -1.7 4,381,852 + +1.4 4,381,852 + Psest_4203 0.34 -1.2 4,381,852 + Psest_4203 0.34 -2.1 4,381,852 + Psest_4203 0.34 +0.0 4,381,852 + Psest_4203 0.34 -1.2 4,381,852 + Psest_4203 0.34 +1.4 4,381,852 + Psest_4203 0.34 -1.2 4,381,853 - Psest_4203 0.34 +0.4 4,381,853 - Psest_4203 0.34 -3.3 4,382,629 + -2.1 4,382,629 + -0.1 4,382,629 + -0.2 4,382,629 + +3.2 4,382,629 + +2.9 4,382,629 + -1.0 4,382,629 + +2.2 4,382,629 + -0.6 4,382,630 - +0.9 4,382,630 - +0.6 4,382,630 - +1.6 4,382,630 - +0.9 4,382,630 - -0.6 4,382,659 + Psest_4204 0.17 +0.6 4,382,659 + Psest_4204 0.17 +0.0 4,382,659 + Psest_4204 0.17 -0.2 4,382,659 + Psest_4204 0.17 -2.2 4,382,660 - Psest_4204 0.17 -0.1 4,382,660 - Psest_4204 0.17 -2.8 4,382,660 - Psest_4204 0.17 -1.4 4,382,660 - Psest_4204 0.17 -1.6 4,382,660 - Psest_4204 0.17 +1.8 4,382,660 - Psest_4204 0.17 +0.4 4,382,660 - Psest_4204 0.17 -0.0 4,382,722 - Psest_4204 0.37 +1.4 4,382,920 + -1.2 4,382,953 + -0.1 4,382,953 + -1.8 4,382,954 - +0.0 4,382,954 - +1.4 4,383,059 + +0.7 4,383,060 - +3.4 4,383,122 - Psest_4205 0.14 +0.6 4,383,242 + Psest_4205 0.22 -0.2 4,383,409 + Psest_4205 0.34 +1.0 4,383,409 + Psest_4205 0.34 -2.2 4,383,410 - Psest_4205 0.34 +0.4 4,383,410 - Psest_4205 0.34 +1.8 4,383,410 - Psest_4205 0.34 +2.2 4,383,508 + Psest_4205 0.41 +1.9 4,383,508 + Psest_4205 0.41 +0.3
Or see this region's nucleotide sequence