Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0406

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0404 and Psest_0405 overlap by 4 nucleotidesPsest_0405 and Psest_0406 overlap by 41 nucleotidesPsest_0406 and Psest_0407 are separated by 83 nucleotides Psest_0404: Psest_0404 - phytoene desaturase, at 439,233 to 440,738 _0404 Psest_0405: Psest_0405 - Phytoene/squalene synthetase, at 440,735 to 441,661 _0405 Psest_0406: Psest_0406 - Fatty acid hydroxylase superfamily., at 441,621 to 442,154 _0406 Psest_0407: Psest_0407 - Dioxygenases related to 2-nitropropane dioxygenase, at 442,238 to 443,281 _0407 Position (kb) 441 442 443Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.622 kb on + strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.623 kb on - strandat 440.624 kb on + strandat 440.849 kb on + strand, within Psest_0405at 440.849 kb on + strand, within Psest_0405at 440.975 kb on + strand, within Psest_0405at 440.975 kb on + strand, within Psest_0405at 440.976 kb on - strand, within Psest_0405at 441.099 kb on - strand, within Psest_0405at 441.099 kb on - strand, within Psest_0405at 441.099 kb on - strand, within Psest_0405at 441.131 kb on + strand, within Psest_0405at 441.131 kb on + strand, within Psest_0405at 441.291 kb on - strand, within Psest_0405at 441.349 kb on - strand, within Psest_0405at 441.407 kb on + strand, within Psest_0405at 441.411 kb on - strand, within Psest_0405at 441.791 kb on + strand, within Psest_0406at 441.838 kb on + strand, within Psest_0406at 441.839 kb on - strand, within Psest_0406at 441.839 kb on - strand, within Psest_0406at 441.886 kb on + strand, within Psest_0406at 441.886 kb on + strand, within Psest_0406at 441.898 kb on + strand, within Psest_0406at 441.994 kb on + strand, within Psest_0406at 441.994 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.075 kb on + strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.076 kb on - strand, within Psest_0406at 442.137 kb on - strandat 442.154 kb on + strandat 442.154 kb on + strandat 442.155 kb on - strandat 442.155 kb on - strandat 442.231 kb on + strandat 442.495 kb on + strand, within Psest_0407at 442.566 kb on + strand, within Psest_0407at 442.577 kb on + strand, within Psest_0407at 442.578 kb on - strand, within Psest_0407at 442.578 kb on - strand, within Psest_0407at 442.578 kb on - strand, within Psest_0407at 442.578 kb on - strand, within Psest_0407at 442.583 kb on + strand, within Psest_0407at 442.584 kb on - strand, within Psest_0407at 443.091 kb on + strand, within Psest_0407at 443.091 kb on + strand, within Psest_0407at 443.091 kb on + strand, within Psest_0407at 443.092 kb on - strand, within Psest_0407

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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440,622 + -0.1
440,622 + -0.2
440,622 + -1.5
440,622 + +1.3
440,622 + +0.3
440,622 + +0.3
440,622 + -0.3
440,622 + +0.3
440,622 + -0.5
440,623 - -0.7
440,623 - +2.0
440,623 - +2.3
440,623 - +0.0
440,623 - -1.3
440,623 - +0.9
440,623 - +0.6
440,623 - -0.3
440,623 - +1.3
440,623 - +1.3
440,623 - +0.1
440,623 - +0.8
440,624 + +0.9
440,849 + Psest_0405 0.12 -1.0
440,849 + Psest_0405 0.12 -1.3
440,975 + Psest_0405 0.26 -1.3
440,975 + Psest_0405 0.26 +0.9
440,976 - Psest_0405 0.26 +1.8
441,099 - Psest_0405 0.39 -0.6
441,099 - Psest_0405 0.39 +2.6
441,099 - Psest_0405 0.39 +2.0
441,131 + Psest_0405 0.43 -0.4
441,131 + Psest_0405 0.43 -2.5
441,291 - Psest_0405 0.60 +1.2
441,349 - Psest_0405 0.66 -0.1
441,407 + Psest_0405 0.72 +0.2
441,411 - Psest_0405 0.73 +2.1
441,791 + Psest_0406 0.32 -0.3
441,838 + Psest_0406 0.41 +1.3
441,839 - Psest_0406 0.41 +0.3
441,839 - Psest_0406 0.41 -1.3
441,886 + Psest_0406 0.50 +0.7
441,886 + Psest_0406 0.50 +1.9
441,898 + Psest_0406 0.52 +2.3
441,994 + Psest_0406 0.70 +1.3
441,994 + Psest_0406 0.70 -0.3
442,075 + Psest_0406 0.85 +2.3
442,075 + Psest_0406 0.85 +2.6
442,075 + Psest_0406 0.85 -0.3
442,075 + Psest_0406 0.85 +0.3
442,075 + Psest_0406 0.85 +0.3
442,075 + Psest_0406 0.85 +0.3
442,075 + Psest_0406 0.85 +1.3
442,075 + Psest_0406 0.85 +2.9
442,075 + Psest_0406 0.85 +0.3
442,075 + Psest_0406 0.85 +2.2
442,075 + Psest_0406 0.85 +0.7
442,075 + Psest_0406 0.85 +0.3
442,075 + Psest_0406 0.85 -0.0
442,076 - Psest_0406 0.85 -0.3
442,076 - Psest_0406 0.85 +1.6
442,076 - Psest_0406 0.85 +0.3
442,076 - Psest_0406 0.85 +0.3
442,076 - Psest_0406 0.85 +0.2
442,076 - Psest_0406 0.85 +1.9
442,076 - Psest_0406 0.85 +0.3
442,076 - Psest_0406 0.85 +1.3
442,076 - Psest_0406 0.85 +1.3
442,076 - Psest_0406 0.85 -2.9
442,076 - Psest_0406 0.85 -0.5
442,076 - Psest_0406 0.85 +2.2
442,076 - Psest_0406 0.85 +2.9
442,137 - -3.1
442,154 + +0.7
442,154 + +1.3
442,155 - +0.7
442,155 - +0.3
442,231 + -0.3
442,495 + Psest_0407 0.25 -0.5
442,566 + Psest_0407 0.31 -3.7
442,577 + Psest_0407 0.32 -1.5
442,578 - Psest_0407 0.33 -0.9
442,578 - Psest_0407 0.33 +1.3
442,578 - Psest_0407 0.33 -0.6
442,578 - Psest_0407 0.33 +1.3
442,583 + Psest_0407 0.33 +0.7
442,584 - Psest_0407 0.33 +0.7
443,091 + Psest_0407 0.82 -0.6
443,091 + Psest_0407 0.82 -0.0
443,091 + Psest_0407 0.82 -1.3
443,092 - Psest_0407 0.82 +2.4

Or see this region's nucleotide sequence