Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4078 and Psest_4079 are separated by 124 nucleotides Psest_4079 and Psest_4080 are separated by 16 nucleotides Psest_4080 and Psest_4081 are separated by 39 nucleotides
Psest_4078: Psest_4078 - L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D, at 4,254,100 to 4,255,083
_4078
Psest_4079: Psest_4079 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 4,255,208 to 4,256,017
_4079
Psest_4080: Psest_4080 - Carbonic anhydrase, at 4,256,034 to 4,256,732
_4080
Psest_4081: Psest_4081 - Glutaminase (EC 3.5.1.2) (from data) , at 4,256,772 to 4,257,680
_4081
Position (kb)
4256
4257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4255.170 kb on + strand at 4255.171 kb on - strand at 4255.181 kb on + strand at 4255.182 kb on - strand at 4255.182 kb on - strand at 4255.260 kb on + strand at 4255.260 kb on + strand at 4255.261 kb on - strand at 4255.261 kb on - strand at 4255.261 kb on - strand at 4255.261 kb on - strand at 4255.292 kb on + strand, within Psest_4079 at 4255.292 kb on + strand, within Psest_4079 at 4255.292 kb on + strand, within Psest_4079 at 4255.293 kb on - strand, within Psest_4079 at 4255.306 kb on - strand, within Psest_4079 at 4255.405 kb on + strand, within Psest_4079 at 4255.430 kb on + strand, within Psest_4079 at 4255.430 kb on + strand, within Psest_4079 at 4255.431 kb on - strand, within Psest_4079 at 4255.431 kb on - strand, within Psest_4079 at 4255.466 kb on + strand, within Psest_4079 at 4255.467 kb on - strand, within Psest_4079 at 4255.467 kb on - strand, within Psest_4079 at 4255.859 kb on + strand, within Psest_4079 at 4255.879 kb on + strand, within Psest_4079 at 4255.879 kb on + strand, within Psest_4079 at 4255.880 kb on - strand, within Psest_4079 at 4255.880 kb on - strand, within Psest_4079 at 4255.973 kb on + strand at 4255.973 kb on + strand at 4255.973 kb on + strand at 4256.047 kb on + strand at 4256.119 kb on + strand, within Psest_4080 at 4256.119 kb on + strand, within Psest_4080 at 4256.119 kb on + strand, within Psest_4080 at 4256.120 kb on - strand, within Psest_4080 at 4256.149 kb on + strand, within Psest_4080 at 4256.149 kb on + strand, within Psest_4080 at 4256.149 kb on + strand, within Psest_4080 at 4256.150 kb on - strand, within Psest_4080 at 4256.150 kb on - strand, within Psest_4080 at 4256.582 kb on + strand, within Psest_4080 at 4256.582 kb on + strand, within Psest_4080 at 4256.648 kb on - strand, within Psest_4080 at 4256.648 kb on - strand, within Psest_4080 at 4256.750 kb on - strand at 4256.968 kb on + strand, within Psest_4081 at 4256.969 kb on - strand, within Psest_4081 at 4257.124 kb on + strand, within Psest_4081 at 4257.124 kb on + strand, within Psest_4081 at 4257.124 kb on + strand, within Psest_4081 at 4257.124 kb on + strand, within Psest_4081 at 4257.125 kb on - strand, within Psest_4081 at 4257.125 kb on - strand, within Psest_4081 at 4257.125 kb on - strand, within Psest_4081 at 4257.125 kb on - strand, within Psest_4081 at 4257.181 kb on + strand, within Psest_4081 at 4257.181 kb on + strand, within Psest_4081 at 4257.181 kb on + strand, within Psest_4081 at 4257.182 kb on - strand, within Psest_4081 at 4257.215 kb on - strand, within Psest_4081 at 4257.610 kb on + strand at 4257.610 kb on + strand at 4257.611 kb on - strand at 4257.611 kb on - strand at 4257.611 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 4,255,170 + +0.4 4,255,171 - +0.4 4,255,181 + -1.0 4,255,182 - -1.2 4,255,182 - -2.1 4,255,260 + -0.5 4,255,260 + -0.0 4,255,261 - -2.1 4,255,261 - -2.6 4,255,261 - +0.6 4,255,261 - +0.3 4,255,292 + Psest_4079 0.10 -1.0 4,255,292 + Psest_4079 0.10 +0.3 4,255,292 + Psest_4079 0.10 -1.7 4,255,293 - Psest_4079 0.10 -1.0 4,255,306 - Psest_4079 0.12 -1.8 4,255,405 + Psest_4079 0.24 +0.4 4,255,430 + Psest_4079 0.27 +0.1 4,255,430 + Psest_4079 0.27 -1.7 4,255,431 - Psest_4079 0.28 +0.5 4,255,431 - Psest_4079 0.28 -2.5 4,255,466 + Psest_4079 0.32 -0.2 4,255,467 - Psest_4079 0.32 -1.3 4,255,467 - Psest_4079 0.32 -2.3 4,255,859 + Psest_4079 0.80 -0.9 4,255,879 + Psest_4079 0.83 +2.1 4,255,879 + Psest_4079 0.83 +1.3 4,255,880 - Psest_4079 0.83 +0.2 4,255,880 - Psest_4079 0.83 -2.1 4,255,973 + -1.3 4,255,973 + -0.6 4,255,973 + +1.7 4,256,047 + +5.7 4,256,119 + Psest_4080 0.12 +1.3 4,256,119 + Psest_4080 0.12 +0.2 4,256,119 + Psest_4080 0.12 -1.8 4,256,120 - Psest_4080 0.12 +0.6 4,256,149 + Psest_4080 0.16 +2.2 4,256,149 + Psest_4080 0.16 +0.2 4,256,149 + Psest_4080 0.16 -2.6 4,256,150 - Psest_4080 0.17 -1.0 4,256,150 - Psest_4080 0.17 +0.0 4,256,582 + Psest_4080 0.78 -1.2 4,256,582 + Psest_4080 0.78 +0.0 4,256,648 - Psest_4080 0.88 -1.0 4,256,648 - Psest_4080 0.88 -3.0 4,256,750 - -0.6 4,256,968 + Psest_4081 0.22 +0.9 4,256,969 - Psest_4081 0.22 +1.4 4,257,124 + Psest_4081 0.39 -1.5 4,257,124 + Psest_4081 0.39 -1.3 4,257,124 + Psest_4081 0.39 -3.3 4,257,124 + Psest_4081 0.39 -0.6 4,257,125 - Psest_4081 0.39 -1.6 4,257,125 - Psest_4081 0.39 -0.8 4,257,125 - Psest_4081 0.39 -0.9 4,257,125 - Psest_4081 0.39 -3.7 4,257,181 + Psest_4081 0.45 -0.3 4,257,181 + Psest_4081 0.45 +1.8 4,257,181 + Psest_4081 0.45 +0.1 4,257,182 - Psest_4081 0.45 -1.7 4,257,215 - Psest_4081 0.49 -2.5 4,257,610 + +0.1 4,257,610 + -0.6 4,257,611 - +0.2 4,257,611 - +0.8 4,257,611 - +0.9
Or see this region's nucleotide sequence