Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0396

Experiment: Polymyxin B sulfate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0395 and Psest_0396 are separated by 38 nucleotidesPsest_0396 and Psest_0397 are separated by 90 nucleotides Psest_0395: Psest_0395 - His Kinase A (phosphoacceptor) domain./Response regulator receiver domain./Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase., at 424,695 to 425,885 _0395 Psest_0396: Psest_0396 - Uncharacterized conserved protein, at 425,924 to 427,384 _0396 Psest_0397: Psest_0397 - Small-conductance mechanosensitive channel, at 427,475 to 430,765 _0397 Position (kb) 425 426 427 428Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 424.980 kb on + strand, within Psest_0395at 424.980 kb on + strand, within Psest_0395at 424.980 kb on + strand, within Psest_0395at 424.980 kb on + strand, within Psest_0395at 424.981 kb on - strand, within Psest_0395at 424.981 kb on - strand, within Psest_0395at 424.981 kb on - strand, within Psest_0395at 425.079 kb on + strand, within Psest_0395at 425.079 kb on + strand, within Psest_0395at 425.080 kb on - strand, within Psest_0395at 426.020 kb on + strandat 426.020 kb on + strandat 426.020 kb on + strandat 426.020 kb on + strandat 426.020 kb on + strandat 426.048 kb on + strandat 426.048 kb on + strandat 426.090 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.105 kb on + strand, within Psest_0396at 426.106 kb on - strand, within Psest_0396at 426.106 kb on - strand, within Psest_0396at 426.106 kb on - strand, within Psest_0396at 426.106 kb on - strand, within Psest_0396at 426.106 kb on - strand, within Psest_0396at 426.106 kb on - strand, within Psest_0396at 426.288 kb on + strand, within Psest_0396at 426.289 kb on - strand, within Psest_0396at 426.406 kb on - strand, within Psest_0396at 426.406 kb on - strand, within Psest_0396at 426.406 kb on - strand, within Psest_0396at 426.406 kb on - strand, within Psest_0396at 426.525 kb on + strand, within Psest_0396at 426.588 kb on + strand, within Psest_0396at 426.588 kb on + strand, within Psest_0396at 426.589 kb on - strand, within Psest_0396at 426.589 kb on - strand, within Psest_0396at 426.589 kb on - strand, within Psest_0396at 426.810 kb on + strand, within Psest_0396at 426.811 kb on - strand, within Psest_0396at 426.811 kb on - strand, within Psest_0396at 426.811 kb on - strand, within Psest_0396at 426.813 kb on + strand, within Psest_0396at 426.813 kb on + strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 426.814 kb on - strand, within Psest_0396at 427.131 kb on + strand, within Psest_0396at 427.131 kb on + strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.132 kb on - strand, within Psest_0396at 427.156 kb on - strand, within Psest_0396at 427.156 kb on - strand, within Psest_0396at 427.280 kb on + strandat 427.280 kb on + strandat 427.280 kb on + strandat 427.280 kb on + strandat 427.281 kb on - strandat 427.281 kb on - strandat 427.403 kb on + strandat 427.404 kb on - strandat 427.435 kb on + strandat 427.779 kb on + strandat 427.779 kb on + strandat 427.779 kb on + strandat 427.779 kb on + strandat 427.780 kb on - strandat 427.780 kb on - strandat 427.780 kb on - strandat 428.249 kb on + strand, within Psest_0397at 428.364 kb on + strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397at 428.365 kb on - strand, within Psest_0397

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Per-strain Table

Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml
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424,980 + Psest_0395 0.24 +1.3
424,980 + Psest_0395 0.24 +1.8
424,980 + Psest_0395 0.24 -0.4
424,980 + Psest_0395 0.24 -0.3
424,981 - Psest_0395 0.24 +1.6
424,981 - Psest_0395 0.24 +0.7
424,981 - Psest_0395 0.24 +0.9
425,079 + Psest_0395 0.32 +1.1
425,079 + Psest_0395 0.32 +0.6
425,080 - Psest_0395 0.32 -0.7
426,020 + +0.3
426,020 + -2.6
426,020 + +1.7
426,020 + +1.5
426,020 + -1.0
426,048 + +0.7
426,048 + +0.3
426,090 + Psest_0396 0.11 -0.7
426,105 + Psest_0396 0.12 +1.6
426,105 + Psest_0396 0.12 -0.5
426,105 + Psest_0396 0.12 -0.0
426,105 + Psest_0396 0.12 -1.3
426,105 + Psest_0396 0.12 -0.4
426,105 + Psest_0396 0.12 +1.2
426,105 + Psest_0396 0.12 +1.9
426,106 - Psest_0396 0.12 +0.4
426,106 - Psest_0396 0.12 +3.1
426,106 - Psest_0396 0.12 +2.0
426,106 - Psest_0396 0.12 -1.7
426,106 - Psest_0396 0.12 +0.5
426,106 - Psest_0396 0.12 +0.2
426,288 + Psest_0396 0.25 -3.7
426,289 - Psest_0396 0.25 +1.4
426,406 - Psest_0396 0.33 +2.5
426,406 - Psest_0396 0.33 +0.9
426,406 - Psest_0396 0.33 +0.5
426,406 - Psest_0396 0.33 -0.7
426,525 + Psest_0396 0.41 -0.4
426,588 + Psest_0396 0.45 +2.9
426,588 + Psest_0396 0.45 +0.3
426,589 - Psest_0396 0.46 -0.2
426,589 - Psest_0396 0.46 -1.5
426,589 - Psest_0396 0.46 +1.7
426,810 + Psest_0396 0.61 -0.3
426,811 - Psest_0396 0.61 -0.3
426,811 - Psest_0396 0.61 +2.3
426,811 - Psest_0396 0.61 +0.3
426,813 + Psest_0396 0.61 +1.6
426,813 + Psest_0396 0.61 +1.6
426,814 - Psest_0396 0.61 -3.6
426,814 - Psest_0396 0.61 +0.3
426,814 - Psest_0396 0.61 -0.4
426,814 - Psest_0396 0.61 +0.4
426,814 - Psest_0396 0.61 +1.1
426,814 - Psest_0396 0.61 -0.3
426,814 - Psest_0396 0.61 +2.0
427,131 + Psest_0396 0.83 -2.3
427,131 + Psest_0396 0.83 +0.4
427,132 - Psest_0396 0.83 +0.7
427,132 - Psest_0396 0.83 +0.2
427,132 - Psest_0396 0.83 +0.3
427,132 - Psest_0396 0.83 +2.5
427,132 - Psest_0396 0.83 +0.8
427,156 - Psest_0396 0.84 +0.3
427,156 - Psest_0396 0.84 +0.7
427,280 + -0.7
427,280 + +0.4
427,280 + +1.6
427,280 + -2.4
427,281 - +0.9
427,281 - +1.4
427,403 + +0.6
427,404 - +1.0
427,435 + +1.8
427,779 + -2.0
427,779 + -2.4
427,779 + -2.7
427,779 + -1.6
427,780 - +3.2
427,780 - -1.5
427,780 - -0.7
428,249 + Psest_0397 0.24 +1.3
428,364 + Psest_0397 0.27 -3.1
428,365 - Psest_0397 0.27 +0.3
428,365 - Psest_0397 0.27 -2.0
428,365 - Psest_0397 0.27 -1.7
428,365 - Psest_0397 0.27 +0.3

Or see this region's nucleotide sequence