Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3962

Experiment: Polymyxin B sulfate 0.0001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Polymyxin B sulfate 0.0001 mg/ml
4,127,666	-				+0.8
4,127,666	-				+0.6
4,127,721	+				+0.6
4,127,722	-				-1.9
4,127,722	-				+1.1
4,128,024	+		Psest_3961	0.62	-1.9
4,128,025	-		Psest_3961	0.62	+1.3
4,128,045	+		Psest_3961	0.65	+0.8
4,128,045	+		Psest_3961	0.65	+0.3
4,128,045	+		Psest_3961	0.65	-0.9
4,128,046	-		Psest_3961	0.65	+2.8
4,128,046	-		Psest_3961	0.65	-0.1
4,128,046	-		Psest_3961	0.65	+0.2
4,128,135	+		Psest_3961	0.81	-1.4
4,128,135	+		Psest_3961	0.81	-1.7
4,128,135	+		Psest_3961	0.81	+2.1
4,128,135	+		Psest_3961	0.81	-0.7
4,128,135	+		Psest_3961	0.81	+1.1
4,128,135	+		Psest_3961	0.81	-1.9
4,128,135	+		Psest_3961	0.81	-1.5
4,128,135	+		Psest_3961	0.81	-0.1
4,128,135	+		Psest_3961	0.81	-2.1
4,128,135	+		Psest_3961	0.81	-0.3
4,128,136	-		Psest_3961	0.81	-2.5
4,128,136	-		Psest_3961	0.81	-0.9
4,128,136	-		Psest_3961	0.81	+1.5
4,128,136	-		Psest_3961	0.81	-1.3
4,128,136	-		Psest_3961	0.81	-2.1
4,128,136	-		Psest_3961	0.81	+1.9
4,128,136	-		Psest_3961	0.81	-1.9
4,128,136	-		Psest_3961	0.81	-1.1
4,128,136	-		Psest_3961	0.81	-0.1
4,128,136	-		Psest_3961	0.81	-1.6
4,128,136	-		Psest_3961	0.81	-1.5
4,128,136	-		Psest_3961	0.81	-1.6
4,128,221	+				-0.5
4,128,221	+				-2.7
4,128,221	+				-0.6
4,128,221	+				-0.7
4,128,221	+				-0.6
4,128,222	-				-1.0
4,128,222	-				-2.3
4,128,222	-				-1.3
4,128,222	-				-1.9
4,128,222	-				-2.0
4,128,222	-				-1.7
4,128,222	-				+1.8
4,128,222	-				-0.6
4,128,222	-				-0.1
4,128,273	+				-1.5
4,128,273	+				+0.4
4,128,284	-				-3.7
4,128,573	-		Psest_3962	0.31	-0.2
4,128,704	-		Psest_3962	0.51	-4.3
4,129,072	+				-0.7
4,129,072	+				+1.5
4,129,072	+				-1.0
4,129,072	+				+1.3
4,129,072	+				-0.0
4,129,072	+				-0.3
4,129,073	-				+1.2
4,129,073	-				-1.2
4,129,073	-				-0.8
4,129,073	-				-1.0
4,129,073	-				-1.0
4,129,073	-				+0.1
4,129,073	-				-1.2
4,129,077	+				-1.9
4,129,077	+				+0.7
4,129,077	+				-2.0
4,129,078	-				+1.1
4,129,078	-				-1.4
4,129,078	-				-0.9
4,129,078	-				-0.6
4,129,176	+				+1.4
4,129,177	-				+2.4
4,129,198	+				-2.3
4,129,199	-				+1.6
4,129,219	+				+0.4
4,129,220	-				+0.1
4,129,220	-				+1.4
4,129,404	+				-2.7
4,129,404	+				-0.8
4,129,404	+				-2.9
4,129,404	+				-3.6
4,129,405	-				-1.8
4,129,540	+		Psest_3963	0.13	-1.3
4,129,719	+		Psest_3963	0.20	+0.8
4,129,719	+		Psest_3963	0.20	+0.9
4,129,719	+		Psest_3963	0.20	-2.1
4,129,719	+		Psest_3963	0.20	-1.2
4,129,719	+		Psest_3963	0.20	-1.2
4,129,719	+		Psest_3963	0.20	-1.7
4,129,720	-		Psest_3963	0.20	-1.9
4,129,720	-		Psest_3963	0.20	-1.3
4,129,720	-		Psest_3963	0.20	-1.3
4,129,720	-		Psest_3963	0.20	-0.6
4,129,829	-		Psest_3963	0.24	-1.5
4,129,847	-		Psest_3963	0.25	-0.5