Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0354 and Psest_0355 are separated by 219 nucleotides Psest_0355 and Psest_0356 are separated by 15 nucleotides
Psest_0354: Psest_0354 - Malate synthase G (EC 2.3.3.9) (from data) , at 379,042 to 381,222
_0354
Psest_0355: Psest_0355 - Glycine cleavage system regulatory protein, at 381,442 to 381,966
_0355
Psest_0356: Psest_0356 - rarD protein, at 381,982 to 382,872
_0356
Position (kb)
381
382 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 380.807 kb on + strand, within Psest_0354 at 380.807 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.981 kb on + strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 381.173 kb on + strand at 381.245 kb on + strand at 381.245 kb on + strand at 381.246 kb on - strand at 381.246 kb on - strand at 381.246 kb on - strand at 381.272 kb on + strand at 381.289 kb on + strand at 381.289 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.386 kb on + strand at 381.386 kb on + strand at 381.387 kb on - strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.389 kb on - strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.393 kb on - strand at 381.528 kb on + strand, within Psest_0355 at 381.629 kb on + strand, within Psest_0355 at 381.713 kb on + strand, within Psest_0355 at 381.996 kb on + strand at 381.996 kb on + strand at 381.996 kb on + strand at 381.997 kb on - strand at 381.997 kb on - strand at 382.025 kb on + strand at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.556 kb on + strand, within Psest_0356 at 382.556 kb on + strand, within Psest_0356 at 382.557 kb on - strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.715 kb on - strand, within Psest_0356 at 382.796 kb on + strand at 382.797 kb on - strand at 382.844 kb on + strand at 382.938 kb on - strand at 382.950 kb on + strand at 382.950 kb on + strand at 382.950 kb on + strand at 382.951 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 380,807 + Psest_0354 0.81 +0.5 380,807 + Psest_0354 0.81 -0.3 380,981 + Psest_0354 0.89 -2.8 380,981 + Psest_0354 0.89 +0.8 380,981 + Psest_0354 0.89 -0.9 380,981 + Psest_0354 0.89 +1.9 380,981 + Psest_0354 0.89 -2.7 380,981 + Psest_0354 0.89 +0.9 380,981 + Psest_0354 0.89 +1.0 380,981 + Psest_0354 0.89 +0.6 380,981 + Psest_0354 0.89 -0.2 380,981 + Psest_0354 0.89 +1.7 380,981 + Psest_0354 0.89 +0.3 380,981 + Psest_0354 0.89 -1.4 380,982 - Psest_0354 0.89 +2.4 380,982 - Psest_0354 0.89 -3.1 380,982 - Psest_0354 0.89 +2.6 380,982 - Psest_0354 0.89 -2.3 380,982 - Psest_0354 0.89 +0.1 381,173 + +1.3 381,245 + -0.7 381,245 + +0.5 381,246 - -1.3 381,246 - +2.3 381,246 - +1.9 381,272 + -2.5 381,289 + +2.1 381,289 + +0.4 381,292 + +1.3 381,292 + +1.7 381,292 + +0.3 381,292 + -0.1 381,292 + +0.3 381,292 + -2.3 381,292 + -1.5 381,293 - -1.3 381,293 - +0.6 381,293 - -3.3 381,293 - -2.1 381,293 - -0.5 381,293 - -1.2 381,293 - -2.1 381,293 - +0.4 381,293 - +2.7 381,293 - +1.0 381,293 - -0.7 381,293 - +0.0 381,293 - +0.4 381,293 - -1.2 381,293 - +1.2 381,293 - -1.0 381,386 + -2.6 381,386 + -0.6 381,387 - +2.5 381,388 + -0.4 381,388 + +2.3 381,388 + +2.3 381,388 + -0.5 381,388 + +2.6 381,388 + -0.7 381,388 + -1.3 381,388 + -2.2 381,388 + +1.3 381,388 + -0.9 381,388 + +0.2 381,389 - +0.9 381,392 + +1.5 381,392 + +0.3 381,392 + +1.6 381,392 + +1.6 381,392 + +0.4 381,393 - -0.3 381,528 + Psest_0355 0.16 -1.1 381,629 + Psest_0355 0.36 -0.7 381,713 + Psest_0355 0.52 -1.5 381,996 + +0.7 381,996 + +1.9 381,996 + +1.0 381,997 - +0.4 381,997 - +1.1 382,025 + -2.3 382,553 + Psest_0356 0.64 -1.5 382,553 + Psest_0356 0.64 +1.3 382,553 + Psest_0356 0.64 -1.5 382,553 + Psest_0356 0.64 +0.3 382,553 + Psest_0356 0.64 +0.3 382,553 + Psest_0356 0.64 +4.4 382,553 + Psest_0356 0.64 +0.2 382,553 + Psest_0356 0.64 +1.1 382,553 + Psest_0356 0.64 -1.5 382,553 + Psest_0356 0.64 -2.2 382,553 + Psest_0356 0.64 +1.5 382,553 + Psest_0356 0.64 -1.3 382,554 - Psest_0356 0.64 -0.7 382,554 - Psest_0356 0.64 -0.6 382,554 - Psest_0356 0.64 -0.3 382,554 - Psest_0356 0.64 +1.3 382,554 - Psest_0356 0.64 +2.2 382,554 - Psest_0356 0.64 +0.0 382,556 + Psest_0356 0.64 +1.7 382,556 + Psest_0356 0.64 -3.0 382,557 - Psest_0356 0.65 -1.8 382,604 + Psest_0356 0.70 +1.3 382,604 + Psest_0356 0.70 +2.3 382,604 + Psest_0356 0.70 -1.5 382,604 + Psest_0356 0.70 +1.4 382,604 + Psest_0356 0.70 +0.5 382,604 + Psest_0356 0.70 +1.5 382,604 + Psest_0356 0.70 -0.3 382,604 + Psest_0356 0.70 -0.5 382,604 + Psest_0356 0.70 -0.7 382,604 + Psest_0356 0.70 +0.8 382,605 - Psest_0356 0.70 +1.6 382,605 - Psest_0356 0.70 -2.0 382,605 - Psest_0356 0.70 +1.4 382,605 - Psest_0356 0.70 -2.2 382,605 - Psest_0356 0.70 -2.6 382,605 - Psest_0356 0.70 +1.1 382,605 - Psest_0356 0.70 +0.6 382,605 - Psest_0356 0.70 +0.9 382,605 - Psest_0356 0.70 +0.7 382,605 - Psest_0356 0.70 +2.3 382,605 - Psest_0356 0.70 -0.1 382,605 - Psest_0356 0.70 -0.3 382,605 - Psest_0356 0.70 -1.0 382,605 - Psest_0356 0.70 -3.1 382,715 - Psest_0356 0.82 -1.8 382,796 + +0.9 382,797 - +1.0 382,844 + -1.0 382,938 - -1.3 382,950 + +2.4 382,950 + +0.5 382,950 + +1.3 382,951 - +1.0
Or see this region's nucleotide sequence