Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0338 and Psest_0339 are separated by 163 nucleotides Psest_0339 and Psest_0340 are separated by 63 nucleotides Psest_0340 and Psest_0341 are separated by 20 nucleotides Psest_0341 and Psest_0342 are separated by 24 nucleotides Psest_0342 and Psest_0343 overlap by 4 nucleotides
Psest_0338: Psest_0338 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 359,798 to 362,008
_0338
Psest_0339: Psest_0339 - hypothetical protein, at 362,172 to 362,501
_0339
Psest_0340: Psest_0340 - hypothetical protein, at 362,565 to 362,747
_0340
Psest_0341: Psest_0341 - flavoprotein, HI0933 family, at 362,768 to 363,952
_0341
Psest_0342: Psest_0342 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 363,977 to 364,843
_0342
Psest_0343: Psest_0343 - hypothetical membrane protein, TIGR01666, at 364,840 to 367,038
_0343
Position (kb)
362
363
364 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 361.984 kb on - strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.581 kb on + strand at 362.581 kb on + strand at 362.582 kb on - strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.866 kb on - strand at 362.866 kb on - strand at 363.023 kb on + strand, within Psest_0341 at 363.023 kb on + strand, within Psest_0341 at 363.024 kb on - strand, within Psest_0341 at 363.027 kb on + strand, within Psest_0341 at 363.027 kb on + strand, within Psest_0341 at 363.027 kb on + strand, within Psest_0341 at 363.027 kb on + strand, within Psest_0341 at 363.028 kb on - strand, within Psest_0341 at 363.028 kb on - strand, within Psest_0341 at 363.028 kb on - strand, within Psest_0341 at 363.188 kb on - strand, within Psest_0341 at 363.188 kb on - strand, within Psest_0341 at 363.214 kb on - strand, within Psest_0341 at 363.221 kb on + strand, within Psest_0341 at 363.222 kb on - strand, within Psest_0341 at 363.222 kb on - strand, within Psest_0341 at 363.222 kb on - strand, within Psest_0341 at 363.222 kb on - strand, within Psest_0341 at 363.222 kb on - strand, within Psest_0341 at 363.222 kb on - strand, within Psest_0341 at 363.314 kb on + strand, within Psest_0341 at 363.329 kb on + strand, within Psest_0341 at 363.676 kb on - strand, within Psest_0341 at 363.724 kb on - strand, within Psest_0341 at 363.724 kb on - strand, within Psest_0341 at 363.786 kb on + strand, within Psest_0341 at 363.786 kb on + strand, within Psest_0341 at 363.786 kb on + strand, within Psest_0341 at 363.786 kb on + strand, within Psest_0341 at 363.787 kb on - strand, within Psest_0341 at 363.787 kb on - strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.788 kb on + strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.789 kb on - strand, within Psest_0341 at 363.957 kb on + strand at 363.957 kb on + strand at 363.957 kb on + strand at 363.957 kb on + strand at 363.957 kb on + strand at 363.958 kb on - strand at 363.958 kb on - strand at 363.982 kb on - strand at 363.982 kb on - strand at 363.982 kb on - strand at 364.122 kb on + strand, within Psest_0342 at 364.122 kb on + strand, within Psest_0342 at 364.123 kb on - strand, within Psest_0342 at 364.162 kb on - strand, within Psest_0342 at 364.162 kb on - strand, within Psest_0342 at 364.192 kb on + strand, within Psest_0342 at 364.192 kb on + strand, within Psest_0342 at 364.192 kb on + strand, within Psest_0342 at 364.193 kb on - strand, within Psest_0342 at 364.193 kb on - strand, within Psest_0342 at 364.193 kb on - strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.274 kb on + strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.275 kb on - strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.278 kb on + strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.279 kb on - strand, within Psest_0342 at 364.325 kb on + strand, within Psest_0342 at 364.326 kb on - strand, within Psest_0342 at 364.326 kb on - strand, within Psest_0342 at 364.326 kb on - strand, within Psest_0342 at 364.367 kb on + strand, within Psest_0342 at 364.368 kb on - strand, within Psest_0342 at 364.480 kb on + strand, within Psest_0342 at 364.481 kb on - strand, within Psest_0342 at 364.481 kb on - strand, within Psest_0342 at 364.491 kb on - strand, within Psest_0342 at 364.496 kb on + strand, within Psest_0342 at 364.496 kb on + strand, within Psest_0342 at 364.496 kb on + strand, within Psest_0342 at 364.496 kb on + strand, within Psest_0342 at 364.496 kb on + strand, within Psest_0342 at 364.496 kb on + strand, within Psest_0342 at 364.497 kb on - strand, within Psest_0342 at 364.497 kb on - strand, within Psest_0342 at 364.497 kb on - strand, within Psest_0342 at 364.497 kb on - strand, within Psest_0342 at 364.497 kb on - strand, within Psest_0342 at 364.539 kb on - strand, within Psest_0342 at 364.539 kb on - strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.572 kb on + strand, within Psest_0342 at 364.573 kb on - strand, within Psest_0342 at 364.573 kb on - strand, within Psest_0342 at 364.573 kb on - strand, within Psest_0342 at 364.747 kb on + strand, within Psest_0342 at 364.748 kb on - strand, within Psest_0342 at 364.791 kb on - strand at 364.842 kb on - strand at 364.948 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 361,984 - -3.1 362,180 + +2.1 362,180 + -0.7 362,180 + -2.5 362,180 + +2.9 362,180 + +0.4 362,180 + +1.3 362,180 + -2.7 362,180 + -1.5 362,180 + +3.4 362,181 - +0.8 362,181 - +1.9 362,181 - +0.2 362,181 - +1.8 362,181 - +0.9 362,181 - -1.7 362,184 + -0.7 362,184 + +1.6 362,184 + +0.3 362,184 + -1.3 362,184 + -0.4 362,185 - +0.5 362,185 - +2.7 362,185 - -0.4 362,185 - -1.7 362,577 + +0.6 362,577 + -1.1 362,577 + -1.3 362,577 + +0.2 362,578 - +0.6 362,578 - -0.4 362,578 - -1.7 362,578 - -2.3 362,578 - -2.2 362,578 - -2.8 362,578 - -1.1 362,578 - +0.6 362,578 - -1.7 362,581 + -1.3 362,581 + -2.4 362,582 - -0.7 362,775 + +1.5 362,775 + -0.7 362,775 + -0.5 362,775 + -2.4 362,775 + -2.1 362,775 + -2.5 362,776 - -4.2 362,776 - -0.8 362,776 - -1.5 362,776 - +1.1 362,776 - +0.2 362,776 - +0.4 362,865 + +0.2 362,865 + -1.4 362,865 + -1.6 362,865 + +1.4 362,865 + -4.3 362,865 + -0.3 362,866 - -0.9 362,866 - -2.2 363,023 + Psest_0341 0.22 -1.3 363,023 + Psest_0341 0.22 -0.7 363,024 - Psest_0341 0.22 -2.3 363,027 + Psest_0341 0.22 +0.4 363,027 + Psest_0341 0.22 -1.1 363,027 + Psest_0341 0.22 -2.0 363,027 + Psest_0341 0.22 -1.8 363,028 - Psest_0341 0.22 +0.1 363,028 - Psest_0341 0.22 -0.1 363,028 - Psest_0341 0.22 -1.9 363,188 - Psest_0341 0.35 -0.9 363,188 - Psest_0341 0.35 -1.5 363,214 - Psest_0341 0.38 +0.3 363,221 + Psest_0341 0.38 +0.9 363,222 - Psest_0341 0.38 +2.3 363,222 - Psest_0341 0.38 +0.5 363,222 - Psest_0341 0.38 -3.5 363,222 - Psest_0341 0.38 -1.3 363,222 - Psest_0341 0.38 +0.0 363,222 - Psest_0341 0.38 -0.0 363,314 + Psest_0341 0.46 +0.8 363,329 + Psest_0341 0.47 -1.5 363,676 - Psest_0341 0.77 -1.5 363,724 - Psest_0341 0.81 -2.6 363,724 - Psest_0341 0.81 +0.1 363,786 + Psest_0341 0.86 -0.3 363,786 + Psest_0341 0.86 +1.1 363,786 + Psest_0341 0.86 -1.1 363,786 + Psest_0341 0.86 +0.4 363,787 - Psest_0341 0.86 +1.5 363,787 - Psest_0341 0.86 +1.6 363,788 + Psest_0341 0.86 -0.9 363,788 + Psest_0341 0.86 -0.6 363,788 + Psest_0341 0.86 +0.9 363,788 + Psest_0341 0.86 +0.5 363,788 + Psest_0341 0.86 +0.3 363,788 + Psest_0341 0.86 -0.8 363,788 + Psest_0341 0.86 -1.3 363,788 + Psest_0341 0.86 -1.6 363,788 + Psest_0341 0.86 -0.3 363,788 + Psest_0341 0.86 +0.9 363,788 + Psest_0341 0.86 +1.1 363,788 + Psest_0341 0.86 -2.7 363,788 + Psest_0341 0.86 -0.3 363,788 + Psest_0341 0.86 -1.3 363,788 + Psest_0341 0.86 +0.1 363,788 + Psest_0341 0.86 -0.2 363,788 + Psest_0341 0.86 +0.0 363,788 + Psest_0341 0.86 +0.0 363,788 + Psest_0341 0.86 -0.3 363,788 + Psest_0341 0.86 +0.3 363,788 + Psest_0341 0.86 +0.7 363,789 - Psest_0341 0.86 +0.7 363,789 - Psest_0341 0.86 -3.0 363,789 - Psest_0341 0.86 -3.3 363,789 - Psest_0341 0.86 -1.3 363,789 - Psest_0341 0.86 +0.4 363,789 - Psest_0341 0.86 -2.1 363,789 - Psest_0341 0.86 +0.4 363,789 - Psest_0341 0.86 -1.5 363,789 - Psest_0341 0.86 +2.3 363,789 - Psest_0341 0.86 -2.1 363,957 + +0.3 363,957 + -1.1 363,957 + -0.7 363,957 + +0.3 363,957 + +0.4 363,958 - +0.2 363,958 - -0.1 363,982 - -2.7 363,982 - +1.2 363,982 - -1.3 364,122 + Psest_0342 0.17 -0.7 364,122 + Psest_0342 0.17 -2.3 364,123 - Psest_0342 0.17 +0.4 364,162 - Psest_0342 0.21 +1.9 364,162 - Psest_0342 0.21 -1.3 364,192 + Psest_0342 0.25 +1.6 364,192 + Psest_0342 0.25 -0.8 364,192 + Psest_0342 0.25 -1.1 364,193 - Psest_0342 0.25 +0.2 364,193 - Psest_0342 0.25 -1.7 364,193 - Psest_0342 0.25 +0.6 364,274 + Psest_0342 0.34 -0.8 364,274 + Psest_0342 0.34 -2.2 364,274 + Psest_0342 0.34 +1.3 364,274 + Psest_0342 0.34 -1.3 364,274 + Psest_0342 0.34 +0.2 364,274 + Psest_0342 0.34 -0.9 364,274 + Psest_0342 0.34 -1.1 364,274 + Psest_0342 0.34 -0.5 364,275 - Psest_0342 0.34 +0.6 364,275 - Psest_0342 0.34 +0.9 364,275 - Psest_0342 0.34 +0.1 364,275 - Psest_0342 0.34 +0.5 364,275 - Psest_0342 0.34 -0.7 364,275 - Psest_0342 0.34 +1.8 364,275 - Psest_0342 0.34 +0.9 364,275 - Psest_0342 0.34 -0.5 364,275 - Psest_0342 0.34 +1.0 364,275 - Psest_0342 0.34 +0.2 364,278 + Psest_0342 0.35 -0.6 364,278 + Psest_0342 0.35 -2.0 364,278 + Psest_0342 0.35 -1.4 364,278 + Psest_0342 0.35 +1.3 364,278 + Psest_0342 0.35 +0.5 364,278 + Psest_0342 0.35 +0.3 364,278 + Psest_0342 0.35 -0.2 364,278 + Psest_0342 0.35 -2.2 364,278 + Psest_0342 0.35 -2.1 364,279 - Psest_0342 0.35 +2.2 364,279 - Psest_0342 0.35 -2.6 364,279 - Psest_0342 0.35 -1.1 364,279 - Psest_0342 0.35 +1.9 364,279 - Psest_0342 0.35 -0.7 364,279 - Psest_0342 0.35 +2.3 364,279 - Psest_0342 0.35 -3.0 364,279 - Psest_0342 0.35 -0.8 364,279 - Psest_0342 0.35 +2.0 364,279 - Psest_0342 0.35 +0.9 364,279 - Psest_0342 0.35 -0.1 364,279 - Psest_0342 0.35 -1.3 364,279 - Psest_0342 0.35 +1.0 364,279 - Psest_0342 0.35 -0.0 364,279 - Psest_0342 0.35 -3.6 364,279 - Psest_0342 0.35 -3.2 364,279 - Psest_0342 0.35 +1.8 364,325 + Psest_0342 0.40 -2.2 364,326 - Psest_0342 0.40 +2.7 364,326 - Psest_0342 0.40 -3.1 364,326 - Psest_0342 0.40 -3.0 364,367 + Psest_0342 0.45 +0.5 364,368 - Psest_0342 0.45 +2.0 364,480 + Psest_0342 0.58 -0.7 364,481 - Psest_0342 0.58 +2.2 364,481 - Psest_0342 0.58 -1.6 364,491 - Psest_0342 0.59 -1.2 364,496 + Psest_0342 0.60 +0.9 364,496 + Psest_0342 0.60 +0.8 364,496 + Psest_0342 0.60 -2.1 364,496 + Psest_0342 0.60 +0.8 364,496 + Psest_0342 0.60 -0.7 364,496 + Psest_0342 0.60 -3.1 364,497 - Psest_0342 0.60 -3.1 364,497 - Psest_0342 0.60 +0.3 364,497 - Psest_0342 0.60 -2.7 364,497 - Psest_0342 0.60 +0.4 364,497 - Psest_0342 0.60 +0.3 364,539 - Psest_0342 0.65 +2.1 364,539 - Psest_0342 0.65 -1.3 364,572 + Psest_0342 0.69 -1.7 364,572 + Psest_0342 0.69 +1.3 364,572 + Psest_0342 0.69 -2.2 364,572 + Psest_0342 0.69 -3.6 364,572 + Psest_0342 0.69 -0.5 364,572 + Psest_0342 0.69 -0.7 364,572 + Psest_0342 0.69 -2.3 364,572 + Psest_0342 0.69 -0.1 364,573 - Psest_0342 0.69 +0.6 364,573 - Psest_0342 0.69 +1.0 364,573 - Psest_0342 0.69 -2.9 364,747 + Psest_0342 0.89 -2.6 364,748 - Psest_0342 0.89 -0.1 364,791 - +0.9 364,842 - -2.6 364,948 + -2.4
Or see this region's nucleotide sequence