Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0327 and Psest_0328 are separated by 95 nucleotides Psest_0328 and Psest_0329 are separated by 107 nucleotides
Psest_0327: Psest_0327 - Transcriptional regulator, at 349,860 to 350,798
_0327
Psest_0328: Psest_0328 - transketolase, bacterial and yeast, at 350,894 to 352,891
_0328
Psest_0329: Psest_0329 - erythrose-4-phosphate dehydrogenase, at 352,999 to 354,045
_0329
Position (kb) 350
351
352
353 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 349.902 kb on + strand at 349.903 kb on - strand at 349.903 kb on - strand at 350.033 kb on - strand, within Psest_0327 at 350.139 kb on - strand, within Psest_0327 at 350.139 kb on - strand, within Psest_0327 at 350.178 kb on - strand, within Psest_0327 at 350.424 kb on - strand, within Psest_0327 at 350.424 kb on - strand, within Psest_0327 at 350.682 kb on + strand, within Psest_0327 at 350.682 kb on + strand, within Psest_0327 at 350.682 kb on + strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.683 kb on - strand, within Psest_0327 at 350.685 kb on + strand, within Psest_0327 at 350.685 kb on + strand, within Psest_0327 at 350.686 kb on - strand, within Psest_0327 at 350.686 kb on - strand, within Psest_0327 at 350.742 kb on + strand at 350.867 kb on + strand at 350.867 kb on + strand at 350.871 kb on + strand at 350.871 kb on + strand at 350.871 kb on + strand at 350.871 kb on + strand at 350.871 kb on + strand at 350.871 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 349,902 + +3.3 349,903 - +0.3 349,903 - +0.2 350,033 - Psest_0327 0.18 -1.5 350,139 - Psest_0327 0.30 +0.9 350,139 - Psest_0327 0.30 +0.1 350,178 - Psest_0327 0.34 +0.3 350,424 - Psest_0327 0.60 +0.9 350,424 - Psest_0327 0.60 +1.9 350,682 + Psest_0327 0.88 +1.4 350,682 + Psest_0327 0.88 +0.8 350,682 + Psest_0327 0.88 +0.8 350,683 - Psest_0327 0.88 -2.2 350,683 - Psest_0327 0.88 +1.9 350,683 - Psest_0327 0.88 -1.1 350,683 - Psest_0327 0.88 -1.3 350,683 - Psest_0327 0.88 +0.8 350,683 - Psest_0327 0.88 -1.5 350,683 - Psest_0327 0.88 +1.5 350,683 - Psest_0327 0.88 -1.0 350,683 - Psest_0327 0.88 +0.2 350,683 - Psest_0327 0.88 +2.0 350,683 - Psest_0327 0.88 +0.7 350,685 + Psest_0327 0.88 +1.8 350,685 + Psest_0327 0.88 -4.0 350,686 - Psest_0327 0.88 +0.2 350,686 - Psest_0327 0.88 +0.7 350,742 + -0.2 350,867 + -0.3 350,867 + -0.3 350,871 + -2.0 350,871 + +1.3 350,871 + -3.8 350,871 + +1.9 350,871 + +0.9 350,871 + +2.1
Or see this region's nucleotide sequence