Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3159 and Psest_3160 are separated by 125 nucleotides Psest_3160 and Psest_3161 are separated by 9 nucleotides Psest_3161 and Psest_3162 are separated by 4 nucleotides
Psest_3159: Psest_3159 - phosphoribosylformylglycinamidine synthase, single chain form, at 3,328,470 to 3,332,366
_3159
Psest_3160: Psest_3160 - ABC-type polar amino acid transport system, ATPase component, at 3,332,492 to 3,333,259
_3160
Psest_3161: Psest_3161 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 3,333,269 to 3,334,366
_3161
Psest_3162: Psest_3162 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 3,334,371 to 3,335,555
_3162
Position (kb)
3333
3334
3335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3332.269 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.411 kb on + strand at 3332.411 kb on + strand at 3332.686 kb on + strand, within Psest_3160 at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.270 kb on + strand at 3333.270 kb on + strand at 3333.271 kb on - strand at 3333.271 kb on - strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.346 kb on - strand at 3333.346 kb on - strand at 3333.366 kb on + strand at 3333.367 kb on - strand at 3333.367 kb on - strand at 3333.367 kb on - strand at 3333.382 kb on + strand, within Psest_3161 at 3333.383 kb on - strand, within Psest_3161 at 3333.383 kb on - strand, within Psest_3161 at 3333.383 kb on - strand, within Psest_3161 at 3333.540 kb on - strand, within Psest_3161 at 3333.585 kb on + strand, within Psest_3161 at 3333.586 kb on - strand, within Psest_3161 at 3333.586 kb on - strand, within Psest_3161 at 3333.586 kb on - strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3334.104 kb on + strand, within Psest_3161 at 3334.105 kb on - strand, within Psest_3161 at 3334.105 kb on - strand, within Psest_3161 at 3334.242 kb on + strand, within Psest_3161 at 3334.243 kb on - strand, within Psest_3161 at 3334.243 kb on - strand, within Psest_3161 at 3334.243 kb on - strand, within Psest_3161 at 3334.354 kb on + strand at 3334.354 kb on + strand at 3334.354 kb on + strand at 3334.354 kb on + strand at 3334.355 kb on - strand at 3334.360 kb on + strand at 3334.360 kb on + strand at 3334.402 kb on + strand at 3334.402 kb on + strand at 3334.402 kb on + strand at 3334.403 kb on - strand at 3334.403 kb on - strand at 3334.403 kb on - strand at 3334.577 kb on - strand, within Psest_3162 at 3334.577 kb on - strand, within Psest_3162 at 3334.741 kb on + strand, within Psest_3162 at 3334.741 kb on + strand, within Psest_3162 at 3334.741 kb on + strand, within Psest_3162 at 3334.742 kb on - strand, within Psest_3162 at 3334.742 kb on - strand, within Psest_3162 at 3335.061 kb on + strand, within Psest_3162 at 3335.062 kb on - strand, within Psest_3162 at 3335.062 kb on - strand, within Psest_3162 at 3335.062 kb on - strand, within Psest_3162 at 3335.122 kb on + strand, within Psest_3162 at 3335.122 kb on + strand, within Psest_3162 at 3335.123 kb on - strand, within Psest_3162 at 3335.123 kb on - strand, within Psest_3162 at 3335.123 kb on - strand, within Psest_3162 at 3335.172 kb on - strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.256 kb on + strand, within Psest_3162 at 3335.314 kb on + strand, within Psest_3162 at 3335.314 kb on + strand, within Psest_3162 at 3335.314 kb on + strand, within Psest_3162 at 3335.315 kb on - strand, within Psest_3162 at 3335.315 kb on - strand, within Psest_3162 at 3335.315 kb on - strand, within Psest_3162
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 3,332,269 + +2.6 3,332,406 + +2.1 3,332,406 + +0.8 3,332,406 + +2.3 3,332,406 + +1.6 3,332,406 + +2.7 3,332,406 + +2.5 3,332,407 - +1.6 3,332,407 - -3.3 3,332,407 - +1.4 3,332,407 - +1.7 3,332,407 - -1.3 3,332,407 - +2.5 3,332,407 - +0.3 3,332,407 - +2.1 3,332,407 - +2.1 3,332,407 - +1.8 3,332,407 - +0.3 3,332,407 - -1.5 3,332,411 + +0.4 3,332,411 + +2.0 3,332,686 + Psest_3160 0.25 +1.7 3,333,259 + -0.0 3,333,259 + +0.4 3,333,259 + -3.1 3,333,259 + -1.0 3,333,259 + -1.4 3,333,259 + +1.4 3,333,259 + -2.4 3,333,259 + -0.8 3,333,259 + +2.3 3,333,259 + -0.5 3,333,259 + +1.8 3,333,259 + -2.8 3,333,259 + +0.9 3,333,259 + +0.7 3,333,259 + +1.1 3,333,260 - +1.2 3,333,260 - +0.5 3,333,260 - -0.7 3,333,260 - -0.6 3,333,260 - -0.5 3,333,260 - +0.7 3,333,260 - -2.4 3,333,260 - -0.2 3,333,260 - +2.6 3,333,260 - -4.0 3,333,260 - -3.1 3,333,260 - -0.2 3,333,270 + -0.6 3,333,270 + -1.0 3,333,271 - -3.8 3,333,271 - -1.0 3,333,345 + -2.6 3,333,345 + +2.8 3,333,345 + +2.1 3,333,345 + -2.5 3,333,345 + -0.7 3,333,346 - -3.0 3,333,346 - +2.1 3,333,366 + +2.0 3,333,367 - +1.5 3,333,367 - +0.9 3,333,367 - -0.4 3,333,382 + Psest_3161 0.10 +1.7 3,333,383 - Psest_3161 0.10 -1.1 3,333,383 - Psest_3161 0.10 +1.5 3,333,383 - Psest_3161 0.10 +1.1 3,333,540 - Psest_3161 0.25 +2.4 3,333,585 + Psest_3161 0.29 +0.5 3,333,586 - Psest_3161 0.29 -2.4 3,333,586 - Psest_3161 0.29 -2.3 3,333,586 - Psest_3161 0.29 +1.4 3,333,636 + Psest_3161 0.33 +3.6 3,333,636 + Psest_3161 0.33 -1.8 3,333,636 + Psest_3161 0.33 +2.4 3,333,636 + Psest_3161 0.33 +0.5 3,333,637 - Psest_3161 0.34 -2.4 3,333,637 - Psest_3161 0.34 +2.1 3,333,637 - Psest_3161 0.34 +1.0 3,333,637 - Psest_3161 0.34 +3.4 3,333,637 - Psest_3161 0.34 -1.8 3,333,637 - Psest_3161 0.34 +1.7 3,334,104 + Psest_3161 0.76 -0.9 3,334,105 - Psest_3161 0.76 +1.0 3,334,105 - Psest_3161 0.76 +0.0 3,334,242 + Psest_3161 0.89 +1.0 3,334,243 - Psest_3161 0.89 -2.6 3,334,243 - Psest_3161 0.89 +1.0 3,334,243 - Psest_3161 0.89 +0.9 3,334,354 + +0.4 3,334,354 + -1.8 3,334,354 + +0.5 3,334,354 + +0.3 3,334,355 - +2.0 3,334,360 + +2.6 3,334,360 + +2.5 3,334,402 + -0.4 3,334,402 + +2.0 3,334,402 + -0.4 3,334,403 - +1.5 3,334,403 - -2.9 3,334,403 - -0.8 3,334,577 - Psest_3162 0.17 -0.9 3,334,577 - Psest_3162 0.17 -2.9 3,334,741 + Psest_3162 0.31 -1.8 3,334,741 + Psest_3162 0.31 +2.6 3,334,741 + Psest_3162 0.31 -1.1 3,334,742 - Psest_3162 0.31 +0.2 3,334,742 - Psest_3162 0.31 -1.1 3,335,061 + Psest_3162 0.58 +3.4 3,335,062 - Psest_3162 0.58 -2.4 3,335,062 - Psest_3162 0.58 +0.4 3,335,062 - Psest_3162 0.58 -1.7 3,335,122 + Psest_3162 0.63 -3.3 3,335,122 + Psest_3162 0.63 +0.3 3,335,123 - Psest_3162 0.63 -1.7 3,335,123 - Psest_3162 0.63 -3.3 3,335,123 - Psest_3162 0.63 -0.2 3,335,172 - Psest_3162 0.68 -3.1 3,335,173 + Psest_3162 0.68 +0.5 3,335,173 + Psest_3162 0.68 +0.3 3,335,173 + Psest_3162 0.68 -0.5 3,335,173 + Psest_3162 0.68 -2.8 3,335,173 + Psest_3162 0.68 +1.1 3,335,173 + Psest_3162 0.68 +0.3 3,335,173 + Psest_3162 0.68 +1.4 3,335,174 - Psest_3162 0.68 +1.3 3,335,174 - Psest_3162 0.68 -0.9 3,335,174 - Psest_3162 0.68 -4.3 3,335,174 - Psest_3162 0.68 +1.1 3,335,174 - Psest_3162 0.68 -3.1 3,335,174 - Psest_3162 0.68 -0.5 3,335,256 + Psest_3162 0.75 +0.7 3,335,314 + Psest_3162 0.80 +0.5 3,335,314 + Psest_3162 0.80 +0.7 3,335,314 + Psest_3162 0.80 -0.4 3,335,315 - Psest_3162 0.80 +0.0 3,335,315 - Psest_3162 0.80 -2.5 3,335,315 - Psest_3162 0.80 -1.0
Or see this region's nucleotide sequence