Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0309 and Psest_0310 are separated by 14 nucleotides Psest_0310 and Psest_0311 overlap by 4 nucleotides
Psest_0309: Psest_0309 - RND family efflux transporter, MFP subunit, at 328,340 to 329,482
_0309
Psest_0310: Psest_0310 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 329,497 to 332,634
_0310
Psest_0311: Psest_0311 - efflux transporter, outer membrane factor (OMF) lipoprotein, NodT family, at 332,631 to 334,055
_0311
Position (kb) 329
330
331
332
333 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 328.736 kb on + strand, within Psest_0309 at 328.926 kb on - strand, within Psest_0309 at 329.162 kb on - strand, within Psest_0309 at 329.491 kb on + strand at 329.491 kb on + strand at 329.491 kb on + strand at 329.965 kb on + strand, within Psest_0310 at 330.104 kb on + strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.105 kb on - strand, within Psest_0310 at 330.413 kb on + strand, within Psest_0310 at 330.414 kb on - strand, within Psest_0310 at 330.475 kb on + strand, within Psest_0310 at 330.563 kb on - strand, within Psest_0310 at 331.126 kb on + strand, within Psest_0310 at 331.126 kb on + strand, within Psest_0310 at 331.858 kb on + strand, within Psest_0310 at 332.164 kb on + strand, within Psest_0310 at 332.564 kb on - strand at 332.577 kb on + strand at 332.577 kb on + strand at 332.577 kb on + strand at 332.577 kb on + strand at 332.748 kb on + strand at 333.015 kb on + strand, within Psest_0311 at 333.016 kb on - strand, within Psest_0311 at 333.097 kb on - strand, within Psest_0311 at 333.222 kb on + strand, within Psest_0311 at 333.223 kb on - strand, within Psest_0311 at 333.337 kb on - strand, within Psest_0311 at 333.472 kb on + strand, within Psest_0311 at 333.473 kb on - strand, within Psest_0311 at 333.473 kb on - strand, within Psest_0311
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 328,736 + Psest_0309 0.35 -1.3 328,926 - Psest_0309 0.51 -3.4 329,162 - Psest_0309 0.72 -2.3 329,491 + +0.3 329,491 + -1.3 329,491 + -0.7 329,965 + Psest_0310 0.15 -0.9 330,104 + Psest_0310 0.19 +0.3 330,105 - Psest_0310 0.19 -2.5 330,105 - Psest_0310 0.19 -2.4 330,105 - Psest_0310 0.19 -1.3 330,105 - Psest_0310 0.19 -1.7 330,105 - Psest_0310 0.19 -1.3 330,413 + Psest_0310 0.29 -0.3 330,414 - Psest_0310 0.29 -1.7 330,475 + Psest_0310 0.31 -0.7 330,563 - Psest_0310 0.34 -2.4 331,126 + Psest_0310 0.52 -1.0 331,126 + Psest_0310 0.52 -0.3 331,858 + Psest_0310 0.75 -1.3 332,164 + Psest_0310 0.85 -1.5 332,564 - -1.3 332,577 + -1.9 332,577 + +0.3 332,577 + +0.6 332,577 + -1.0 332,748 + -1.5 333,015 + Psest_0311 0.27 -1.7 333,016 - Psest_0311 0.27 -0.0 333,097 - Psest_0311 0.33 -1.9 333,222 + Psest_0311 0.41 +0.3 333,223 - Psest_0311 0.42 -0.3 333,337 - Psest_0311 0.50 -1.0 333,472 + Psest_0311 0.59 -0.3 333,473 - Psest_0311 0.59 +0.3 333,473 - Psest_0311 0.59 +1.3
Or see this region's nucleotide sequence