Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3139 and Psest_3140 are separated by 48 nucleotides Psest_3140 and Psest_3141 are separated by 108 nucleotides Psest_3141 and Psest_3142 are separated by 58 nucleotides Psest_3142 and Psest_3143 are separated by 51 nucleotides
Psest_3139: Psest_3139 - Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily, at 3,314,196 to 3,314,717
_3139
Psest_3140: Psest_3140 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 3,314,766 to 3,315,491
_3140
Psest_3141: Psest_3141 - ADP-ribose pyrophosphatase, at 3,315,600 to 3,316,163
_3141
Psest_3142: Psest_3142 - Uncharacterized protein conserved in bacteria, at 3,316,222 to 3,316,725
_3142
Psest_3143: Psest_3143 - Uncharacterized protein conserved in bacteria, at 3,316,777 to 3,317,094
_3143
Position (kb)
3315
3316
3317 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3314.755 kb on - strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.837 kb on - strand at 3314.837 kb on - strand at 3314.837 kb on - strand at 3314.839 kb on - strand, within Psest_3140 at 3314.839 kb on - strand, within Psest_3140 at 3314.883 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.935 kb on + strand, within Psest_3140 at 3315.037 kb on + strand, within Psest_3140 at 3315.037 kb on + strand, within Psest_3140 at 3315.038 kb on - strand, within Psest_3140 at 3315.058 kb on + strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.059 kb on - strand, within Psest_3140 at 3315.216 kb on + strand, within Psest_3140 at 3315.217 kb on - strand, within Psest_3140 at 3315.350 kb on - strand, within Psest_3140 at 3315.369 kb on + strand, within Psest_3140 at 3315.369 kb on + strand, within Psest_3140 at 3315.370 kb on - strand, within Psest_3140 at 3315.370 kb on - strand, within Psest_3140 at 3315.452 kb on - strand at 3315.538 kb on - strand at 3315.607 kb on - strand at 3315.897 kb on + strand, within Psest_3141 at 3315.897 kb on + strand, within Psest_3141 at 3315.897 kb on + strand, within Psest_3141 at 3315.897 kb on + strand, within Psest_3141 at 3315.898 kb on - strand, within Psest_3141 at 3315.898 kb on - strand, within Psest_3141 at 3315.898 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.900 kb on - strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.933 kb on + strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3315.934 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.064 kb on - strand, within Psest_3141 at 3316.257 kb on + strand at 3316.257 kb on + strand at 3316.258 kb on - strand at 3316.287 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.453 kb on + strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.454 kb on - strand, within Psest_3142 at 3316.482 kb on + strand, within Psest_3142 at 3316.482 kb on + strand, within Psest_3142 at 3316.483 kb on - strand, within Psest_3142 at 3316.483 kb on - strand, within Psest_3142 at 3316.483 kb on - strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.486 kb on + strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.487 kb on - strand, within Psest_3142 at 3316.679 kb on + strand at 3316.679 kb on + strand at 3316.680 kb on - strand at 3316.680 kb on - strand at 3316.811 kb on + strand, within Psest_3143 at 3316.812 kb on - strand, within Psest_3143 at 3316.812 kb on - strand, within Psest_3143 at 3316.812 kb on - strand, within Psest_3143 at 3316.858 kb on - strand, within Psest_3143 at 3316.906 kb on + strand, within Psest_3143 at 3316.907 kb on - strand, within Psest_3143 at 3316.936 kb on - strand, within Psest_3143 at 3316.978 kb on + strand, within Psest_3143 at 3317.011 kb on + strand, within Psest_3143 at 3317.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 3,314,755 - -3.0 3,314,836 + -3.7 3,314,836 + -2.6 3,314,836 + +1.2 3,314,836 + +0.2 3,314,837 - +0.8 3,314,837 - -2.5 3,314,837 - +2.8 3,314,839 - Psest_3140 0.10 +0.1 3,314,839 - Psest_3140 0.10 -0.5 3,314,883 + Psest_3140 0.16 -3.3 3,314,917 + Psest_3140 0.21 -0.5 3,314,917 + Psest_3140 0.21 -2.0 3,314,917 + Psest_3140 0.21 +0.5 3,314,917 + Psest_3140 0.21 -2.9 3,314,917 + Psest_3140 0.21 -4.6 3,314,918 - Psest_3140 0.21 +2.4 3,314,918 - Psest_3140 0.21 -0.3 3,314,918 - Psest_3140 0.21 -2.8 3,314,918 - Psest_3140 0.21 +1.3 3,314,918 - Psest_3140 0.21 -2.7 3,314,918 - Psest_3140 0.21 -2.6 3,314,935 + Psest_3140 0.23 +1.4 3,315,037 + Psest_3140 0.37 -3.4 3,315,037 + Psest_3140 0.37 -2.5 3,315,038 - Psest_3140 0.37 -1.5 3,315,058 + Psest_3140 0.40 +0.1 3,315,059 - Psest_3140 0.40 -0.1 3,315,059 - Psest_3140 0.40 +1.2 3,315,059 - Psest_3140 0.40 +2.3 3,315,059 - Psest_3140 0.40 +1.0 3,315,059 - Psest_3140 0.40 -3.2 3,315,216 + Psest_3140 0.62 +1.6 3,315,217 - Psest_3140 0.62 -2.2 3,315,350 - Psest_3140 0.80 -3.9 3,315,369 + Psest_3140 0.83 +0.0 3,315,369 + Psest_3140 0.83 +3.4 3,315,370 - Psest_3140 0.83 -0.3 3,315,370 - Psest_3140 0.83 -2.5 3,315,452 - +1.1 3,315,538 - +1.0 3,315,607 - +0.8 3,315,897 + Psest_3141 0.53 +0.8 3,315,897 + Psest_3141 0.53 -1.4 3,315,897 + Psest_3141 0.53 +0.5 3,315,897 + Psest_3141 0.53 -3.3 3,315,898 - Psest_3141 0.53 +0.9 3,315,898 - Psest_3141 0.53 +1.3 3,315,898 - Psest_3141 0.53 +1.2 3,315,900 - Psest_3141 0.53 +1.6 3,315,900 - Psest_3141 0.53 +1.7 3,315,900 - Psest_3141 0.53 +0.8 3,315,900 - Psest_3141 0.53 -3.2 3,315,933 + Psest_3141 0.59 -3.2 3,315,933 + Psest_3141 0.59 +0.5 3,315,933 + Psest_3141 0.59 -1.4 3,315,933 + Psest_3141 0.59 +1.1 3,315,933 + Psest_3141 0.59 +1.2 3,315,933 + Psest_3141 0.59 -2.0 3,315,933 + Psest_3141 0.59 -0.9 3,315,933 + Psest_3141 0.59 +0.9 3,315,933 + Psest_3141 0.59 +1.6 3,315,933 + Psest_3141 0.59 -0.8 3,315,933 + Psest_3141 0.59 +1.7 3,315,933 + Psest_3141 0.59 -1.0 3,315,933 + Psest_3141 0.59 +2.2 3,315,933 + Psest_3141 0.59 +0.2 3,315,933 + Psest_3141 0.59 -2.8 3,315,933 + Psest_3141 0.59 +1.9 3,315,933 + Psest_3141 0.59 +0.6 3,315,933 + Psest_3141 0.59 +1.1 3,315,933 + Psest_3141 0.59 +0.1 3,315,934 - Psest_3141 0.59 +1.1 3,315,934 - Psest_3141 0.59 +1.3 3,315,934 - Psest_3141 0.59 +2.2 3,315,934 - Psest_3141 0.59 +0.4 3,315,934 - Psest_3141 0.59 +1.7 3,315,934 - Psest_3141 0.59 +0.4 3,315,934 - Psest_3141 0.59 +4.6 3,315,934 - Psest_3141 0.59 +1.1 3,315,934 - Psest_3141 0.59 +0.1 3,315,934 - Psest_3141 0.59 +1.1 3,315,934 - Psest_3141 0.59 +0.0 3,315,934 - Psest_3141 0.59 -0.8 3,315,934 - Psest_3141 0.59 -1.0 3,315,934 - Psest_3141 0.59 +1.9 3,315,934 - Psest_3141 0.59 +0.2 3,315,934 - Psest_3141 0.59 +0.3 3,315,934 - Psest_3141 0.59 +0.1 3,315,934 - Psest_3141 0.59 +0.7 3,316,064 - Psest_3141 0.82 -2.4 3,316,064 - Psest_3141 0.82 -1.1 3,316,064 - Psest_3141 0.82 +1.3 3,316,064 - Psest_3141 0.82 -3.0 3,316,064 - Psest_3141 0.82 +1.8 3,316,064 - Psest_3141 0.82 +1.2 3,316,064 - Psest_3141 0.82 +0.2 3,316,257 + +0.1 3,316,257 + +0.9 3,316,258 - +2.2 3,316,287 + Psest_3142 0.13 +0.6 3,316,453 + Psest_3142 0.46 +2.2 3,316,453 + Psest_3142 0.46 +1.8 3,316,453 + Psest_3142 0.46 +1.0 3,316,453 + Psest_3142 0.46 +1.1 3,316,453 + Psest_3142 0.46 +0.7 3,316,453 + Psest_3142 0.46 -0.6 3,316,453 + Psest_3142 0.46 -2.7 3,316,454 - Psest_3142 0.46 +0.9 3,316,454 - Psest_3142 0.46 +1.0 3,316,454 - Psest_3142 0.46 +2.4 3,316,454 - Psest_3142 0.46 +0.6 3,316,482 + Psest_3142 0.52 +0.9 3,316,482 + Psest_3142 0.52 -3.2 3,316,483 - Psest_3142 0.52 -2.5 3,316,483 - Psest_3142 0.52 +1.8 3,316,483 - Psest_3142 0.52 -0.5 3,316,486 + Psest_3142 0.52 -0.3 3,316,486 + Psest_3142 0.52 +0.1 3,316,486 + Psest_3142 0.52 +1.6 3,316,486 + Psest_3142 0.52 -0.5 3,316,486 + Psest_3142 0.52 +0.9 3,316,486 + Psest_3142 0.52 +1.1 3,316,486 + Psest_3142 0.52 -2.2 3,316,486 + Psest_3142 0.52 +1.6 3,316,486 + Psest_3142 0.52 -3.4 3,316,486 + Psest_3142 0.52 +1.9 3,316,486 + Psest_3142 0.52 -0.6 3,316,486 + Psest_3142 0.52 +0.9 3,316,486 + Psest_3142 0.52 -3.8 3,316,487 - Psest_3142 0.53 +1.2 3,316,487 - Psest_3142 0.53 +0.6 3,316,487 - Psest_3142 0.53 -2.2 3,316,487 - Psest_3142 0.53 +0.8 3,316,487 - Psest_3142 0.53 +2.5 3,316,487 - Psest_3142 0.53 +0.3 3,316,487 - Psest_3142 0.53 +0.2 3,316,487 - Psest_3142 0.53 +1.6 3,316,679 + +0.2 3,316,679 + +1.5 3,316,680 - +1.1 3,316,680 - +0.8 3,316,811 + Psest_3143 0.11 +0.6 3,316,812 - Psest_3143 0.11 -2.6 3,316,812 - Psest_3143 0.11 -2.6 3,316,812 - Psest_3143 0.11 +0.9 3,316,858 - Psest_3143 0.25 -0.3 3,316,906 + Psest_3143 0.41 -0.3 3,316,907 - Psest_3143 0.41 -3.2 3,316,936 - Psest_3143 0.50 -2.9 3,316,978 + Psest_3143 0.63 -1.2 3,317,011 + Psest_3143 0.74 -1.4 3,317,159 + -1.3
Or see this region's nucleotide sequence