Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3136 and Psest_3137 are separated by 30 nucleotides Psest_3137 and Psest_3138 are separated by 44 nucleotides Psest_3138 and Psest_3139 overlap by 4 nucleotides Psest_3139 and Psest_3140 are separated by 48 nucleotides
Psest_3136: Psest_3136 - Putative Mg2+ and Co2+ transporter CorB, at 3,311,465 to 3,312,751
_3136
Psest_3137: Psest_3137 - phosphoribosylglycinamide formyltransferase 2, at 3,312,782 to 3,313,963
_3137
Psest_3138: Psest_3138 - Predicted Fe-S protein, at 3,314,008 to 3,314,199
_3138
Psest_3139: Psest_3139 - Carbonic anhydrases/acetyltransferases, isoleucine patch superfamily, at 3,314,196 to 3,314,717
_3139
Psest_3140: Psest_3140 - NTP pyrophosphohydrolases including oxidative damage repair enzymes, at 3,314,766 to 3,315,491
_3140
Position (kb)
3312
3313
3314 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3311.784 kb on + strand, within Psest_3136 at 3311.784 kb on + strand, within Psest_3136 at 3311.784 kb on + strand, within Psest_3136 at 3312.042 kb on + strand, within Psest_3136 at 3312.153 kb on + strand, within Psest_3136 at 3312.153 kb on + strand, within Psest_3136 at 3312.154 kb on - strand, within Psest_3136 at 3312.154 kb on - strand, within Psest_3136 at 3312.300 kb on - strand, within Psest_3136 at 3312.300 kb on - strand, within Psest_3136 at 3312.378 kb on + strand, within Psest_3136 at 3312.379 kb on - strand, within Psest_3136 at 3312.386 kb on + strand, within Psest_3136 at 3312.524 kb on + strand, within Psest_3136 at 3312.524 kb on + strand, within Psest_3136 at 3312.524 kb on + strand, within Psest_3136 at 3312.525 kb on - strand, within Psest_3136 at 3312.525 kb on - strand, within Psest_3136 at 3312.785 kb on + strand at 3312.786 kb on - strand at 3312.786 kb on - strand at 3312.786 kb on - strand at 3313.094 kb on - strand, within Psest_3137 at 3313.106 kb on - strand, within Psest_3137 at 3313.276 kb on - strand, within Psest_3137 at 3313.658 kb on + strand, within Psest_3137 at 3313.668 kb on + strand, within Psest_3137 at 3313.669 kb on - strand, within Psest_3137 at 3313.731 kb on + strand, within Psest_3137 at 3313.731 kb on + strand, within Psest_3137 at 3313.731 kb on + strand, within Psest_3137 at 3313.732 kb on - strand, within Psest_3137 at 3313.732 kb on - strand, within Psest_3137 at 3313.732 kb on - strand, within Psest_3137 at 3313.732 kb on - strand, within Psest_3137 at 3313.790 kb on + strand, within Psest_3137 at 3313.790 kb on + strand, within Psest_3137 at 3313.790 kb on + strand, within Psest_3137 at 3313.791 kb on - strand, within Psest_3137 at 3313.791 kb on - strand, within Psest_3137 at 3313.791 kb on - strand, within Psest_3137 at 3313.841 kb on + strand, within Psest_3137 at 3313.842 kb on - strand, within Psest_3137 at 3314.009 kb on + strand at 3314.009 kb on + strand at 3314.009 kb on + strand at 3314.010 kb on - strand at 3314.010 kb on - strand at 3314.238 kb on - strand at 3314.245 kb on + strand at 3314.245 kb on + strand at 3314.245 kb on + strand at 3314.246 kb on - strand at 3314.246 kb on - strand at 3314.246 kb on - strand at 3314.440 kb on + strand, within Psest_3139 at 3314.440 kb on + strand, within Psest_3139 at 3314.453 kb on + strand, within Psest_3139 at 3314.453 kb on + strand, within Psest_3139 at 3314.453 kb on + strand, within Psest_3139 at 3314.454 kb on - strand, within Psest_3139 at 3314.454 kb on - strand, within Psest_3139 at 3314.526 kb on - strand, within Psest_3139 at 3314.553 kb on - strand, within Psest_3139 at 3314.755 kb on - strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.836 kb on + strand at 3314.837 kb on - strand at 3314.837 kb on - strand at 3314.837 kb on - strand at 3314.839 kb on - strand, within Psest_3140 at 3314.839 kb on - strand, within Psest_3140 at 3314.883 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.917 kb on + strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.918 kb on - strand, within Psest_3140 at 3314.935 kb on + strand, within Psest_3140
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 3,311,784 + Psest_3136 0.25 -2.7 3,311,784 + Psest_3136 0.25 +0.9 3,311,784 + Psest_3136 0.25 +0.6 3,312,042 + Psest_3136 0.45 +1.0 3,312,153 + Psest_3136 0.53 -4.4 3,312,153 + Psest_3136 0.53 -4.1 3,312,154 - Psest_3136 0.54 -3.9 3,312,154 - Psest_3136 0.54 +0.6 3,312,300 - Psest_3136 0.65 -1.0 3,312,300 - Psest_3136 0.65 -4.0 3,312,378 + Psest_3136 0.71 +1.6 3,312,379 - Psest_3136 0.71 -1.9 3,312,386 + Psest_3136 0.72 -0.1 3,312,524 + Psest_3136 0.82 +0.9 3,312,524 + Psest_3136 0.82 +1.8 3,312,524 + Psest_3136 0.82 +2.6 3,312,525 - Psest_3136 0.82 -2.8 3,312,525 - Psest_3136 0.82 +2.5 3,312,785 + -1.1 3,312,786 - +2.3 3,312,786 - +0.0 3,312,786 - -0.8 3,313,094 - Psest_3137 0.26 +0.7 3,313,106 - Psest_3137 0.27 +0.7 3,313,276 - Psest_3137 0.42 -4.4 3,313,658 + Psest_3137 0.74 +0.2 3,313,668 + Psest_3137 0.75 -1.3 3,313,669 - Psest_3137 0.75 -3.7 3,313,731 + Psest_3137 0.80 -3.6 3,313,731 + Psest_3137 0.80 -2.1 3,313,731 + Psest_3137 0.80 -4.0 3,313,732 - Psest_3137 0.80 -1.1 3,313,732 - Psest_3137 0.80 +1.0 3,313,732 - Psest_3137 0.80 +1.3 3,313,732 - Psest_3137 0.80 -0.2 3,313,790 + Psest_3137 0.85 -1.7 3,313,790 + Psest_3137 0.85 -1.5 3,313,790 + Psest_3137 0.85 -1.6 3,313,791 - Psest_3137 0.85 -1.1 3,313,791 - Psest_3137 0.85 -0.9 3,313,791 - Psest_3137 0.85 +1.1 3,313,841 + Psest_3137 0.90 -3.7 3,313,842 - Psest_3137 0.90 +1.1 3,314,009 + +1.5 3,314,009 + +1.3 3,314,009 + -3.1 3,314,010 - -2.5 3,314,010 - -3.8 3,314,238 - -0.9 3,314,245 + +0.1 3,314,245 + +0.5 3,314,245 + -1.0 3,314,246 - -0.2 3,314,246 - -4.2 3,314,246 - -0.8 3,314,440 + Psest_3139 0.47 +0.4 3,314,440 + Psest_3139 0.47 +2.0 3,314,453 + Psest_3139 0.49 -0.7 3,314,453 + Psest_3139 0.49 -2.8 3,314,453 + Psest_3139 0.49 -2.3 3,314,454 - Psest_3139 0.49 -0.8 3,314,454 - Psest_3139 0.49 -0.9 3,314,526 - Psest_3139 0.63 -3.4 3,314,553 - Psest_3139 0.68 +1.4 3,314,755 - -3.0 3,314,836 + -3.7 3,314,836 + -2.6 3,314,836 + +1.2 3,314,836 + +0.2 3,314,837 - +0.8 3,314,837 - -2.5 3,314,837 - +2.8 3,314,839 - Psest_3140 0.10 +0.1 3,314,839 - Psest_3140 0.10 -0.5 3,314,883 + Psest_3140 0.16 -3.3 3,314,917 + Psest_3140 0.21 -0.5 3,314,917 + Psest_3140 0.21 -2.0 3,314,917 + Psest_3140 0.21 +0.5 3,314,917 + Psest_3140 0.21 -2.9 3,314,917 + Psest_3140 0.21 -4.6 3,314,918 - Psest_3140 0.21 +2.4 3,314,918 - Psest_3140 0.21 -0.3 3,314,918 - Psest_3140 0.21 -2.8 3,314,918 - Psest_3140 0.21 +1.3 3,314,918 - Psest_3140 0.21 -2.7 3,314,918 - Psest_3140 0.21 -2.6 3,314,935 + Psest_3140 0.23 +1.4
Or see this region's nucleotide sequence