Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0290 and Psest_0291 overlap by 1 nucleotides Psest_0291 and Psest_0292 are separated by 57 nucleotides Psest_0292 and Psest_0293 are separated by 22 nucleotides
Psest_0290: Psest_0290 - dihydroorotase, multifunctional complex type, at 305,220 to 306,494
_0290
Psest_0291: Psest_0291 - aspartate carbamoyltransferase, at 306,494 to 307,510
_0291
Psest_0292: Psest_0292 - Pyrimidine operon attenuation protein/uracil phosphoribosyltransferase, at 307,568 to 308,080
_0292
Psest_0293: Psest_0293 - RNAse H-fold protein YqgF, at 308,103 to 308,531
_0293
Position (kb) 306
307
308 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 305.659 kb on - strand, within Psest_0290 at 305.987 kb on - strand, within Psest_0290 at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.732 kb on + strand, within Psest_0291 at 306.732 kb on + strand, within Psest_0291 at 306.741 kb on + strand, within Psest_0291 at 307.482 kb on - strand at 307.545 kb on + strand at 307.545 kb on + strand at 307.557 kb on + strand at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.667 kb on + strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 307.668 kb on - strand, within Psest_0292 at 308.022 kb on + strand, within Psest_0292
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 305,659 - Psest_0290 0.34 -1.5 305,987 - Psest_0290 0.60 -1.7 306,595 - +2.3 306,595 - -2.4 306,595 - -0.7 306,732 + Psest_0291 0.23 -3.6 306,732 + Psest_0291 0.23 -2.3 306,741 + Psest_0291 0.24 -2.2 307,482 - -0.5 307,545 + -1.1 307,545 + +0.3 307,557 + -1.6 307,667 + Psest_0292 0.19 +1.3 307,667 + Psest_0292 0.19 -0.4 307,667 + Psest_0292 0.19 -3.0 307,668 - Psest_0292 0.19 +1.2 307,668 - Psest_0292 0.19 +0.1 307,668 - Psest_0292 0.19 -0.3 308,022 + Psest_0292 0.88 -0.8
Or see this region's nucleotide sequence