Experiment: Polymyxin B sulfate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0288 and Psest_0289 are separated by 133 nucleotides Psest_0289 and Psest_0290 are separated by 67 nucleotides Psest_0290 and Psest_0291 overlap by 1 nucleotides
Psest_0288: Psest_0288 - putative DNA modification/repair radical SAM protein, at 303,412 to 304,623
_0288
Psest_0289: Psest_0289 - Predicted membrane protein, at 304,757 to 305,152
_0289
Psest_0290: Psest_0290 - dihydroorotase, multifunctional complex type, at 305,220 to 306,494
_0290
Psest_0291: Psest_0291 - aspartate carbamoyltransferase, at 306,494 to 307,510
_0291
Position (kb) 305
306
307 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 304.268 kb on + strand, within Psest_0288 at 304.269 kb on - strand, within Psest_0288 at 304.269 kb on - strand, within Psest_0288 at 304.410 kb on - strand, within Psest_0288 at 304.421 kb on + strand, within Psest_0288 at 304.422 kb on - strand, within Psest_0288 at 304.422 kb on - strand, within Psest_0288 at 304.577 kb on + strand at 304.577 kb on + strand at 304.577 kb on + strand at 304.577 kb on + strand at 304.578 kb on - strand at 304.639 kb on - strand at 304.639 kb on - strand at 304.639 kb on - strand at 304.639 kb on - strand at 304.651 kb on + strand at 304.651 kb on + strand at 304.652 kb on - strand at 304.652 kb on - strand at 304.657 kb on - strand at 304.657 kb on - strand at 304.774 kb on + strand at 304.774 kb on + strand at 304.775 kb on - strand at 304.791 kb on - strand at 304.832 kb on + strand, within Psest_0289 at 304.832 kb on + strand, within Psest_0289 at 304.832 kb on + strand, within Psest_0289 at 304.833 kb on - strand, within Psest_0289 at 304.835 kb on + strand, within Psest_0289 at 304.970 kb on + strand, within Psest_0289 at 304.971 kb on - strand, within Psest_0289 at 304.987 kb on - strand, within Psest_0289 at 305.017 kb on - strand, within Psest_0289 at 305.017 kb on - strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.027 kb on + strand, within Psest_0289 at 305.028 kb on - strand, within Psest_0289 at 305.028 kb on - strand, within Psest_0289 at 305.028 kb on - strand, within Psest_0289 at 305.057 kb on + strand, within Psest_0289 at 305.097 kb on - strand, within Psest_0289 at 305.188 kb on + strand at 305.188 kb on + strand at 305.189 kb on - strand at 305.189 kb on - strand at 305.659 kb on - strand, within Psest_0290 at 305.987 kb on - strand, within Psest_0290 at 306.595 kb on - strand at 306.595 kb on - strand at 306.595 kb on - strand at 306.732 kb on + strand, within Psest_0291 at 306.732 kb on + strand, within Psest_0291 at 306.741 kb on + strand, within Psest_0291 at 307.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 0.0001 mg/ml remove 304,268 + Psest_0288 0.71 -1.8 304,269 - Psest_0288 0.71 -0.7 304,269 - Psest_0288 0.71 +0.3 304,410 - Psest_0288 0.82 +0.4 304,421 + Psest_0288 0.83 -0.7 304,422 - Psest_0288 0.83 -2.5 304,422 - Psest_0288 0.83 +0.4 304,577 + -0.3 304,577 + +1.3 304,577 + -2.3 304,577 + -0.2 304,578 - +0.7 304,639 - +2.1 304,639 - -1.8 304,639 - -0.4 304,639 - +1.5 304,651 + -0.3 304,651 + +0.5 304,652 - -0.0 304,652 - +0.1 304,657 - -0.1 304,657 - -1.6 304,774 + +0.6 304,774 + +0.6 304,775 - -1.4 304,791 - -1.4 304,832 + Psest_0289 0.19 +2.0 304,832 + Psest_0289 0.19 -1.1 304,832 + Psest_0289 0.19 -1.3 304,833 - Psest_0289 0.19 +0.8 304,835 + Psest_0289 0.20 -0.7 304,970 + Psest_0289 0.54 -1.0 304,971 - Psest_0289 0.54 -1.4 304,987 - Psest_0289 0.58 +0.3 305,017 - Psest_0289 0.66 +0.1 305,017 - Psest_0289 0.66 +0.5 305,027 + Psest_0289 0.68 -1.8 305,027 + Psest_0289 0.68 -0.8 305,027 + Psest_0289 0.68 -0.5 305,027 + Psest_0289 0.68 +1.8 305,027 + Psest_0289 0.68 -0.3 305,027 + Psest_0289 0.68 -1.0 305,027 + Psest_0289 0.68 +0.8 305,027 + Psest_0289 0.68 -1.0 305,027 + Psest_0289 0.68 +0.4 305,027 + Psest_0289 0.68 -0.4 305,028 - Psest_0289 0.68 +0.5 305,028 - Psest_0289 0.68 -0.7 305,028 - Psest_0289 0.68 -2.3 305,057 + Psest_0289 0.76 +1.3 305,097 - Psest_0289 0.86 -0.3 305,188 + -0.7 305,188 + -0.4 305,189 - +0.5 305,189 - +0.7 305,659 - Psest_0290 0.34 -1.5 305,987 - Psest_0290 0.60 -1.7 306,595 - +2.3 306,595 - -2.4 306,595 - -0.7 306,732 + Psest_0291 0.23 -3.6 306,732 + Psest_0291 0.23 -2.3 306,741 + Psest_0291 0.24 -2.2 307,482 - -0.5
Or see this region's nucleotide sequence